Università degli Studi di Perugia

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Teaching: In vitro culture

Degree CourseDegree course in Agricultural and Environmental Sciences [L-25] D. M. 270/2004
LocationPerugia
CurriculumBIOTECNOLOGIE - Regulations 2014
PrerequisitesInformation currently not available - Italian version
Assessment methods

Oral exam and Written exam with open answer questions in itinere

Examinations' grades statisticsInformation currently not available - Italian version
Examinations' dates

www.agr.unipg.it

Recommended optional coursesInformation currently not available - Italian version
LecturerTiziano GARDI
TypeSpecific formative activities
AreaDISCIPLINE DELLA PRODUZIONE VEGETALE
SectorAGR/03
ECTS6
Implementation proceduresConventional
Course content

Definition and meaning of in vitro culture. Methods of regeneration. Bases morphological, physiological and biochemical in vitro morphogenesis. Regeneration and cell totipotency. Micropropagation and cloning in vitro. Stages of micropropagation. Encapsulation technology. Propagules. Capsule. Synthetic seed. Possible applications of micropropagation and encapsulation technology. Procedures for in vitro conservation of germplasm. Laboratory activities: Handling aseptically growing media, Removal of meristematic apexes, preparation capsules and synthetic seeds with different types of vitro-derived explants.

Supplement

Description of In vitro cultures. Regeneration methods. Morphological, physiological and biochemical bases of in vitro morphogenesis. Cellular totipotency and  in vitro regeneration. Micropropagation phases. Encapsulation and synthetic seeds. Storage and cryoconservation.

Mode of delivery

face-to-fac and Practical training in laboratory

Recommended reading

- "MICROPROPAGAZIONE DELLE PIANTE ORTOFLOROFRUTTICOLE". G. De Paoli, V. Rossi, A. Scozzoli - EDAGRICOLE-Edizioni Agricole della Calderini s.r.l. - Bologna, 1994.

- "VEGETATIVE PROPAGATION. VITRO CULTURE OF HIGHER PLANTS". Pierik R.L.M. - Martinus Nijhoff Publisher - Dordrecht - The Netherlands, 1987.

Learning outcomes

Far acquisire allo studente le tecniche della micropropagazione e le epoche di prelievo del materiale vegetale da porre in vitro; le procedure di sterilizzazione ed il prelievo di apici meristematici in condizioni di asepsi.

Teaching period

www.agr.unipg.it

Teaching calendar

www.agr.unipg.it

Teaching supportInformation currently not available - Italian version
Teaching languageItalian
Attendance

Optional

Seat

Agricultural Faculty - In vitro culture laboratory

Hours
Theoretical
45
Practical
0
Individual study
90
Integration to teaching activity
15
Tiziano GARDI
Total
150
Year3
PeriodI semester
NotesInformation currently not available - Italian version
Consulting hours

Contact with the teacher; e-mail: tiziano.gardi@unipg.it

Consulting location

Department of Agricultural, Food and Environmental Sciences

ECTS code2014 - 1585

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