Università degli Studi di Perugia

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Unit APPLIED BIOCHEMISTRY METHODS

Course
Pharmacy
Study-unit Code
65105410
Location
PERUGIA
Curriculum
In all curricula
Teacher
Carmela Conte
Teachers
  • Carmela Conte - Didattica Ufficiale
  • Michela Codini - Didattica Ufficiale (Codocenza)
Hours
  • 42 ore - Didattica Ufficiale - Carmela Conte
  • 21 ore - Didattica Ufficiale (Codocenza) - Michela Codini
CFU
9
Course Regulation
Coorte 2016
Offered
2018/19
Learning activities
Caratterizzante
Area
Discipline biologiche e farmacologiche
Sector
BIO/10
Type of study-unit
Obbligatorio (Required)
Type of learning activities
Attività formativa monodisciplinare
Language of instruction
English
Contents
Biochemical bases of hormonal action. Molecular bases of metabolic diseases. Methods of execution and biomedical meaning of the main biochemical clinical analyzes.
Reference texts
- Patti, Contestabile e Di Salvo. Metodologie Biochimiche di
Casa Editrice Ambrosiana
- Italo Antonozzi e Elio Gulletta
Medicina di Laboratorio
Piccin
- Giorgio Federici
Medicina di Laboratorio
Mc Graw-Hill
Educational objectives
At the end of the study the student will be able to acquire the basic knowledge of the fundamental principles of biochemical methodologies in the biomedical field.
Prerequisites
The course aims to address issues concerning the Biochemical Applied Methodologies that the students will be able to face after acquisition of the basic knowledge of General and Systematic Biochemistry. It is essential that the student have acquired the knowledges of structure and function of biological macromolecules and basic mechanisms underlying many processes of cell regulation.
Teaching methods
The course is organized as follows: Lectures on all subjects gf the course
Learning verification modality
The test requires a single oral exam during which the student will answer questions concerning issues of the lectures and listed in the program on the teaching. The proof test includes several questions with open answer. During the interview the student will demonstrate consistency with the question referred, clarity and accuracy of scientific terminology, and ability to transfer knowledge even through graphic representations.
Extended program
-Biochemical methods:
-General principles of biochemical methodologies (i.e. designing, performing an experiment and interpreting data)
- Protein separation and purification techniques
- purification from biological sources (cells, tissues)
- fractionation of mixtures (centrifugation, precipitation)
- chromatography (ion exchange, affinity, breakdown, adsorption, HPLC)
- Dialysis and ultrafiltration
- Protein analysis and quantification techniques
- Electrophoresis (SDS-PAGE, Isoelectrofocusing, 2D-PAGE and applications)
- quantitative dosages (spectrophotometric, UV-VIS, fluorescence, luminometry)
- Blotting
- Isolation of nucleic acids
- Analysis of gene expression: PCR and RealTime-PCR, DNA microarray-
- Production of recombinant proteins.
- Cloning
- purification of recombinant proteins

-Enzymes and clinical enzymology:
- Enzyme classification
- Enzymatic kinetics
- Enzyme inhibition
- Enzymes in diagnostics (diagnostic indicators in chemical-clinical analyzes, methods for determining the related enzymatic activities, immunoenzymatic methods)
- Determination of enzymatic activity
Laboratory Medicine
Hemochromocytometric analysis
- Plasma proteins
- Evaluation of glucose metabolism
- Evaluation of lipid metabolism
- Evaluation of liver function
- Evaluation of renal function
- Analysis of Urine
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