Università degli Studi di Perugia

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Unit LABORATORY OF BIOLOGY II (INTERDISCIPLINARY)

Course
Biological sciences
Study-unit Code
55A00906
Curriculum
In all curricula
Teacher
Ines Di Rosa
Teachers
  • Ines Di Rosa - Didattica Ufficiale
Hours
  • 24 ore - Didattica Ufficiale - Ines Di Rosa
CFU
2
Course Regulation
Coorte 2016
Offered
2018/19
Learning activities
Altro
Area
Altre conoscenze utili per l'inserimento nel mondo del lavoro
Sector
BIO/06
Type of study-unit
Obbligatorio (Required)
Type of learning activities
Attività formativa monodisciplinare
Language of instruction
Italian
Contents
Microscopy and cultivation of bacteria.

Genomic sequence variability and phylogenetic analysis.

Recognition and comparison of microscopic and macroscopic specimens.

Spectrophotometric determination of proteins in cell extracts, including the construction of calibration curve.

Taxonomic identification of plankton species, diversity indices, stress biomarkers.
Reference texts
Equipements provided by the teaching.
Educational objectives
The course aims to provide students the ability to apply the knowledge acquired during each discipline lectures, such as familiarity with the microbiology laboratory, understanding of the molecular basis of genetic transmission, familiarity with the use of some of the main methods applied in the biological chemistry, ability to relate anatomical organization to functional and evolutionary aspects, to acquire skills in taxonomic identification of plankton species using the dichotomous keys and in biochemical methods as prognostic tools of environmental health.
Prerequisites
In order to benefit from the laboratory experiments on the topics listed in the program, students must have acquired basic knowledge on Microbiology, Genetics, Comparative Anatomy, Biological Chemistry, Ecology.
Teaching methods
The course is organized in laboratory experiments regarding the following disciplines: Microbiology, Genetics, Comparative Anatomy, Biochemical Chemistry, Ecology.
Learning verification modality
For the attainment of the credits programmed for this teaching (2 credits-24 hours) it is necessary to attend at least 75% of the total number of hours, therefore at least 18 hours. The attendance will be assessed by roll call and signature capture.

For information on support services for students with disabilities and / or DSA visit the page.
Extended program
MICROBIOLOGY: Laboratory methods for the study of microorganisms. Microscopy, direct and staining methods. Main culture techniques for
bacterial isolation, count and presumptive identification.

GENETICS: Genomic sequence analyses, intra and inter specific variability study for phylogenetic and phylogeographic investigations.

COMPARATIVE ANATOMY: Recognition and comparison of microscopicand macroscopic specimens from embryonic and adult vertebrates.

BIOLOGICAL CHEMISTRY: Spectrophotometric determination of proteins in cell extracts, including the construction of calibration curve.

ECOLOGY: Biodiversity of freshwater plankton based on taxonomic and biochemical investigation: taxonomic identification, application of
diversity indices and measurement of biomarkers of environmental contamination.
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