Università degli Studi di Perugia

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Unit LABORATORY OF CHEMISTRY (INTERDISCIPLINARY)

Course
Biological sciences
Study-unit Code
55030202
Curriculum
In all curricula
Teacher
Oriana Piermatti
Teachers
  • Oriana Piermatti - Didattica Ufficiale
Hours
  • 24 ore - Didattica Ufficiale - Oriana Piermatti
CFU
2
Course Regulation
Coorte 2017
Offered
2018/19
Learning activities
Altro
Area
Altre conoscenze utili per l'inserimento nel mondo del lavoro
Sector
CHIM/06
Type of study-unit
Obbligatorio (Required)
Type of learning activities
Attività formativa monodisciplinare
Language of instruction
Italian
Contents
Prevention and safety in the chemistry laboratory. Separation and purification techniques of chemical compounds: filtration, extraction, recrystallization, distillation and chromatography. Determinations of acidity/basicity constants by acid-base titration. Practical exercises are planned in the laboratory.
Reference texts
1.C. Gilbert, S. F. Martin; Chimica Organica Sperimentale, Zanichelli
2.Teacher lectures;
Educational objectives
Must be learned the fundamental techniques for the purification and separation of organic compounds and principles that are the basis of these techniques, the determination of the concentration by volumetric analysis.

At the end of the course the student should be able to prepare solutions of known concentration, to determine the concentration of unknown solutions, perform a filtration, a TLC analysis and a column chromatography.
Prerequisites
In order to understand the topics you need to have good knowledge of the basic concepts of general chemistry about the chemical bonding, acidity and alkalinity, equilibrium constants.
Teaching methods
The course is organized as follows:
- Lectures on all subjects of the course
- Practical laboratory exercises: students will perform four experiments: 1) preparation of solutions with defined concentration; 2) Determination of concentration of acetic acid by the acid-base titration; 3) Preparation of phenyl benzoate: separation, TLC analysis and characterization by melting point; 4) Separation of ortho- and para-nitrophenol by column chromatograpy.
Other information
Frequency:
Required
Learning verification modality
No examination
Extended program
Prevention and safety in the chemistry laboratory. Separation and purification techniques of chemical compounds. Filtration: simple and under vacuum, vacuum pumps. Extraction: the partition coefficient, selective extraction, discontinuous and continuous extraction. Recrystallization and melting point. Distillation: simple and fractional. Chromatography: theoretical principles, thin layer chromatography, column chromatography, gas chromatography and HPLC. Determination of acidity/basicity constants by acid-base titration. Volumetric analysis. pH indicators.

Practical exercises are planned in the laboratory regarding the preparation of solutions with defined concentration, the acid-base titration, the thin layer chromatography and column chromatography.
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