Università degli Studi di Perugia

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Unit BIOMEDICAL SPECTROSCOPY

Course
Physics
Study-unit Code
GP005501
Curriculum
Fisica medica
Teacher
Daniele Fioretto
Teachers
  • Daniele Fioretto - Didattica Ufficiale
Hours
  • 47 ore - Didattica Ufficiale - Daniele Fioretto
CFU
6
Course Regulation
Coorte 2017
Offered
2018/19
Learning activities
Affine/integrativa
Area
Attività formative affini o integrative
Sector
FIS/03
Type of study-unit
Opzionale (Optional)
Type of learning activities
Attività formativa monodisciplinare
Language of instruction
italian
Contents
Optics and optical and spectroscopic instruments. Biomedical microscopy methods. Introduction to light scattering: photocorrelation spectroscopy, Brillouin spectroscopy, Raman spectroscopy. IR spectroscopy and fluorescence from biological matter. Spectroscopic mapping.
Reference texts
Lecture notes, articles and chapters of textbooks indicated therein.
Educational objectives
The main objective of the course is to provide students with the basic knowledge to understand the theoretical foundations and some experimental methods of spectroscopy applied in the biomedical field. The main skills that the course aims to transmit are: - know how to use the fundamental physical laws to interpret some basic aspects of spectroscopy, structure, dynamics and function of biological molecules - know how to analyze some spectroscopic experiments typical of biomedical spectroscopy, its potential and limits.
Prerequisites
In order to understand the contents of the course it is advisable to have acquired basic knowledge of Physics of Matter and of Molecular Biophysics.
Teaching methods
There will be lectures on all the topics covered in the course and laboratory experiments concerning photorrelation spectroscopies, Brillouin, Raman, IR and spectroscopic imaging.
Learning verification modality
The exam consists in the presentation of individual seminars by the students on topics related to the course and in an oral exam. The oral exam consists of a discussion lasting about 30 minutes aimed at ascertaining the level of knowledge on the technical and methodological contents of the course.
Extended program
Optics and optical instruments. Spatial resolution of the microscopes, Rayleigh criterion and numerical aperture. Frequency resolution of spectrometers: gratings and Fabry-Perot. Biomedical microscopy methods. Introduction to light scattering. Simple hydrodynamics, Brillouin spectrum and damped harmonic oscillator. Raman spectroscopy: experiments and elaborations. Raman spectroscopy applied to biological matter. Brillouin spectroscopy in presence of relaxation processes. IR spectroscopy from biological matter. IR spectra processing techniques. Fluorescence from biological matter. Hydrodynamics and diffusion processes. Photocorrelation spectroscopy. Dynamics of soft matter: experiments, models, molecular dynamics. Generalized hydrodynamics. Brillouin spectroscopy in presence of relaxation processes.
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