Università degli Studi di Perugia

Skip to content

Unit MOLECULAR TOOLS FOR FAUNISTIC ANALYSIS

Course
Natural and environmental sciences and technologies
Study-unit Code
55320706
Curriculum
In all curricula
Teacher
Livia Lucentini
Teachers
  • Livia Lucentini - Didattica Ufficiale
Hours
  • 42 ore - Didattica Ufficiale - Livia Lucentini
CFU
6
Course Regulation
Coorte 2017
Offered
2018/19
Learning activities
Caratterizzante
Area
Discipline biologiche
Sector
BIO/06
Type of study-unit
Obbligatorio (Required)
Type of learning activities
Attività formativa monodisciplinare
Language of instruction
Italian
Contents
Introduction to the use of molecular markers in faunistic managment. resolution of taxonomic ambiguity and application of both nuclear and mt molecular markers. National and local examples.
Reference texts
Power point and word files provided.


Population Genetics for Animal Conservation. CAMBRIDGE.
Educational objectives
Comprehension of the use of molecular markers in faunistic managment. resolution of taxonomic ambiguity and application of both nuclear and mt molecular markers. National and local examples. Manipulation and comprehension of biomolecular data
Prerequisites
nd
Teaching methods
theoretical and laboratory lessons. Computer simulations.
Other information
Attendance is strongly recommended
Learning verification modality
Written test and/or oral exam
Extended program
Introduction to the use of molecular tools in faunistic management. Molecular methodologies as PCR, restriction enzymes, PCR-RFLP, AFLP, microsatellite, DNA Barcoding and SNPs: theory and practical applications. Microsatellite enriched genomic library. Museum historical collections: importance as scientific tool. DNA extraction from conservative and historical samples. Hardy-Weinberg equilibrium. Mutation and migration indexes. Selection and fitness. Inbreeding. Coefficients I, F, F statistics. On-line databases. Resolution of taxonomic ambiguity - practical examples: trout. Molecular evaluation of a species complex: Baetis rhodani complex. Conservative approach to Perdix in repopulation stock.Molecular evidences on Squalus albus. Relationships between AF, environmental data and molecular characterization. PCR-RFLP as a tool to individuate food mystifications. Practical applications: cladograms, phylograms. Local molecular taxonomy: Esox flaviae.
Go top
Table of content
×