Unit MOLECULAR BIOLOGY

Course
Biological sciences
Study-unit Code
55077608
Curriculum
In all curricula
Teacher
Sabata Martino
Teachers
  • Sabata Martino - Didattica Ufficiale
Hours
  • 56 ore - Didattica Ufficiale - Sabata Martino
CFU
8
Course Regulation
Coorte 2016
Offered
2018/19
Learning activities
Caratterizzante
Area
Discipline biomolecolari
Sector
BIO/11
Type of study-unit
Obbligatorio (Required)
Type of learning activities
Attività formativa monodisciplinare
Language of instruction
Italian
Contents
Biological macromolecules. Genome, transcriptome and proteome. Nucleic acids: expression and use of the information contained therein. Recombinant DNA technologies. Use of internet and databases in molecular biology
Reference texts
J.D.Watson, T.A.Baker,S.P.Bell, A.G.Gann, M.Levine,
R.Losick: Biologia Molecolare del Gene, 7a Ed. Zanichelli

B. Lewin: Il Gene X, Ed.Zanichelli

T.A.Brown: Biotecnologie Molecolari-Principi e tecniche Zanichelli

Materiale didattico fornito dal docente
Educational objectives
The aim is to provide the basic knowledge of the structure and biosynthesis of biological macromolecules: DNA, RNA and proteins; to make students able to correlate structure and function of macromolecules and understand the functioning of cells at the molecular level; to provide the basic knowledge on experimental tools that are basic for molecular biology.
Prerequisites
In order to be able to understand the course with highest profit, student must have general knowledge of biology, anatomy and chemistry acquired in the school curriculum
Teaching methods
Lectures will be made by using slides and movies. Some lessons could be dedicated to revision and depth of issues also proposed by the students and that could involve students themselves. Some practical lessons will be taught in the classroom and laboratory of bioinformatics.
Other information
It is planned a tutorial activity during the course and for students who request help in the preparation of the exam. The dates of receiving students are available in the professor's website.
Learning verification modality
The final examination consists of an oral exam on the subjects of the course. To pass the exam, students must have achieved at least a sufficient preparation both for Module of General biology and Module of Histology and Embriology. During the course, progress assessments consisting of written tests will be proposed. In this way students will be able of evaluate their acquired knowledge and to fill any eventual learning gaps.
Extended program
Biological macromolecules.
Proteins, structure and functions (overview)
Enzymes (outline)
The double helix structure of DNA.
Organization of eukaryotic chromatin.
DNA replication.
Mechanisms of DNA repair
Mechanisms of DNA recombination.
Retroviruses and retrotransposons.
Structure and function of bacterial RNA polymerase
Basic mechanism of regulation of transcription in prokaryotes
Structure and function of eukaryotic RNA polymerase.
Study of eukaryotic promoters and transcription units.
Basic mechanism and regulation of transcription in eukaryotes.
The maturation of transcripts and splicing mechanism. RNA editing.
Ribozymes and RNA "interference"
Protein synthesis in prokaryotes and eukaryotes
Recombinant DNA technology: methods of extraction and analysis of nucleic acids
Plasmid vectors, phage, cosmids; vectors for specialized applications;
Construction, cloning and selection of the recombinant DNA.
The products of recombinant DNA technology.
Principles of bioinformatics for Molecular Biology. Use of databases and analysis of nucleotide sequences and amino acid