Unit ANIMAL BREEDING

Course
Animal science
Study-unit Code
GP000824
Curriculum
In all curricula
Teacher
Francesca Maria Sarti
CFU
9
Course Regulation
Coorte 2018
Offered
2018/19
Type of study-unit
Obbligatorio (Required)
Type of learning activities
Attività formativa integrata

GENETICS OF AGRICULTURALLY IMPORTANTORGANISMS

Code GP000830
CFU 3
Teacher Francesca Maria Sarti
Teachers
  • Gianpiero Marconi - Didattica Ufficiale (Codocenza)
Hours
  • 30 ore - Didattica Ufficiale (Codocenza) - Gianpiero Marconi
Learning activities Affine/integrativa
Area Attività formative affini o integrative
Sector AGR/07
Type of study-unit Obbligatorio (Required)
Language of instruction Italian
Contents Nucleic acids structures. Duplication, transcription and translation. Molecular technologies applied to animal breeding. Practicals: DNA extraction and purification; PCR reactions.
Reference texts i) Study material provided by the teacher. ii) Weising, Nybom, Wolff, Kahl. DNA Fingerprinting in Plants Principles, Methods, and Applications. CRC Press. iii) Lorenzetti, Ceccarelli, Rosellini, Veronesi - Genetica Agraria - Pàtroni editore; iv) Dale, Schantz and Plant. Dai geni ai Genomi – Edises editore; v) Giulio Pagnacco. Genetica animale.
Educational objectives The main objective of lectures is to provide at the students basics knowledge to analyze and evaluate the genetic variability present in breding animals through nucleic acids analysis perfomed by molecular techniques. The main knowledge gained will be:- Acquisition of molecular biotechnology techniques;- Molecular data analysis;- The student should be able to properly move in a biotechnology laboratory.
Prerequisites Not required
Teaching methods The course is organized as follows:- Classroom lectures about all course's topic;- Lab practical applications of developed techniques in the classroom;- Seminars on the topics covered.
Other information Lecture participation Recommended
Learning verification modality Oral exam
Extended program Nucleic acids; DNA replication, transciption, translation; gene structure and expression; restriction enzimes; polymerase chain reaction (PCR); electrophoresis; molecular markers RAPD, AFLP, SSR, STS( CAPS and PCR-RFLP), SNP, GBS; Genome Wide Association Study (GWAS), Sanger and NGS DNA squencing; use of molecular techniques in breeding.

ANIMAL BREEDING

Code GP000829
CFU 6
Teacher Francesca Maria Sarti
Teachers
  • Francesca Maria Sarti - Didattica Ufficiale
Hours
  • 60 ore - Didattica Ufficiale - Francesca Maria Sarti
Learning activities Caratterizzante
Area Discipline zootecniche e delle produzioni animali
Sector AGR/17
Type of study-unit Obbligatorio (Required)
Language of instruction Italian
Contents Estimation of breeding value; selection programmes; biotechnologies applied to animal breeding; animal biodiversity.
Reference texts 1. G. PAGNACCO Genetica animale. Applicazioni zootecniche e veterinarie. Seconda edizione. Casa Ed. Ambrosiana, Milano, 2016.
2. D. VAN VLECK, J. POLLACK, E. OLTENACU Genetica per le scienze animali, SEU, Pisa, 1998.
3. R.M. BOURDON Understanding animal breeding, Prentice Hall Inc., Upper Saddle River (NJ), 1997.
4. A.M. PILLA Valutazione genetica e scelta degli animali, Edagricole, 2002.
5. Barcaccia & Falcinelli Genetica e genomica vol. III Liguori Editore.
6. P .J. RUSSELL Genetica, EdiSES, 2007.
7.https://wiki.groenkennisnet.nl/display/TAB/Textbook+Animal+Breeding+and+Genetics
8. Online presentations.
Educational objectives Provide a depth knowledge of livestock aimed at defining genetic selection plans, the application of biodiversity conservation methods in order to be able to define conservation plans of endangered breeds, also by means of molecular genetic techniques.
Prerequisites Biometrics applied to animal sciences
Teaching methods The course is organized as follows:
front -lessons classroom on the topics listed in the program;
guided exercises with the computer aid on the topics explained during the course;
final exercise with the final written test simulation.
Learning verification modality The examination consists in a final written exam lasting about three hours in which students must demonstrate that they can calculate breeding value, relationship and genetic indexes of sire.
Extended program Introduction to animal breeding
Recall quantitative traits, qualitative and threshold, the infinitesimal genetic model, estimate the correct phenotype, breeding value and the value of the gene combination.
Estimate of genetic indexes
Calculation of genetic indexes single trait, pedigree index, multiple trait index, accuracy of the estimate and confidence interval, Producing Ability, BLUP estimates: sire and animal models, normal distribution and its utility in defining strategies for the selection, calculation of progress genetic.
Genetic evaluation of sire (performance testing, progeny testing, sib test). Biodiversity animal genetic drift, small animal populations, conservation techniques (in situ and ex situ), calculation of the actual number, examples of conservation.
PRACTICAL LESSONS
Calculation of single genetic trait, multiple-trait, pedigree, Producing Ability, estimation accuracy and genetic progress, resolution of models for estimating indices BLUP sire and animal in a manual and with the aid of computer tools (Excel and software R).