Unit ADVANCED MOLECULAR BIOLOGY
- Course
- Pharmaceutical biotechnologies
- Study-unit Code
- GP003556
- Location
- PERUGIA
- Curriculum
- In all curricula
- Teacher
- Mariangela Morlando
- Teachers
-
- Mariangela Morlando
- Hours
- 52 ore - Mariangela Morlando
- CFU
- 6
- Course Regulation
- Coorte 2022
- Offered
- 2022/23
- Learning activities
- Caratterizzante
- Area
- Discipline biotecnologiche comuni
- Academic discipline
- BIO/11
- Type of study-unit
- Obbligatorio (Required)
- Type of learning activities
- Attività formativa monodisciplinare
- Language of instruction
- Italian
- Contents
- Advanced knowledge on mechanisms of gene expression regulation in Eukaryotes at transcritpional and post-transcriptional levels and on experimental tools used for their study. Advanced methodologies for molecular studies on wide scale manly at genomic, transcriptomic and epitranscriptomic. All these acquired knowledge will facilitate the understanding of molecular mechanisms in physiological and pathological conditions using modern advanced approaches.
- Reference texts
- Biologia molecolare - Zlatanova, van Holde - 2018
Biologia molecolare del gene Watson, Baker, Bell, Gann, Levine, Losick - edizione 2022
Genetica e Genomica nell scienze mediche. Strachan-Goodship-Chinnery
Biotecnologie Molecolari Brown - second edition 2017
Scientific papers and teaching material provided by the Professor though the Unistudium platform - Educational objectives
- The course aims to provide a solid advanced knowledge on the activity of genes and genomes and on the mechanisms of gene regulation at transcriptional and post-transcriptional levels. Moreover, the course provides training tools for the execution of analytical-instrumental procedures necessary for carrying out biological investigations in vivo, in vitro and in silico.
- Prerequisites
- Basic knowledge of molecular and cellular biology
- Teaching methods
- Lectures will be made by using slides and movies. Wet labs in order to put into practice the theoretical knowledge.
- Other information
- Learning verification modality
- oral exam aimed to assess the knowledge acquired and the ability to understand the topics of the course and of a scientific work selected by the student; therefore, communication skills and the use of proper scientific language will be assessed.
- Extended program
- Structure and function of human genome: the genomic era
Gene expression control: the epigenetic and transcriptomic analysis; non coding RNAs; the epitranscriptome.
Functional genetic variability and polymorphisms
Next-generation sequencing methodologies.
Experimental approaches for the study of DNA: DNAse I sensibility assay; study of DNA methylation by Methyl-seq; Cromatin IP also combined with DNA seq; Chromosome conformation capture; Southern blot, PCR.
Recombinant DNA: basic principles and applications.
Experimental approaches for the study of gene expression: Transcriptomic analyses by next generation sequencing; Northern blot, RT-PCR, Real-time PCR. Reporter genes. In situ hybridization, Western blot.
RNA-protein interaction: EMSA, RNA pull down, RIP, CLIP.
Genome editing (CRISPR-Cas). Site specific mutagenesis: Knock-out, Knock-in and knock-down.
Cellular and animal model systems