Unit APPLIED BIOLOGY AND BASIC AND MOLECULAR GENETICS OF BIODRUGS
- Course
- Pharmaceutical biotechnologies
- Study-unit Code
- GP003554
- Location
- PERUGIA
- Curriculum
- In all curricula
- Teacher
- Rita Romani
- CFU
- 12
- Course Regulation
- Coorte 2022
- Offered
- 2022/23
- Type of study-unit
- Obbligatorio (Required)
- Type of learning activities
- Attività formativa integrata
BASIC AND MOLECULAR GENETICS OF BIODRUGS
| Code | GP003562 |
|---|---|
| Location | PERUGIA |
| CFU | 6 |
| Teacher | Marco Gargaro |
| Teachers |
|
| Hours |
|
| Learning activities | Caratterizzante |
| Area | Discipline biotecnologiche comuni |
| Academic discipline | BIO/18 |
| Type of study-unit | Obbligatorio (Required) |
| Language of instruction | Italian |
| Contents | The course consists of lectures and practical exercises aimed at learning genetic engineering techniques for the production of new biotechnological drugs such as recombinant proteins and enzymes. |
| Reference texts | R.J. Reece- Analisi dei geni e dei genomi. EdiSES. |
| Educational objectives | The course of "Genetic and molecular basis of biodrugs" aims at giving to the students the skills to design and realize a biodrug. Theoric classes will give the fundamental notions to identify potential new biodrugs and to modify phisiologic proteins for a proper customization of the therapy. Laboratory classes aims at giving the skills for cloning, translating and verifying the biologic activity of a therapeutic fusion protein. |
| Prerequisites | Fundamentals of biology and pharmacology. |
| Teaching methods | Theorical and practical lessons. |
| Other information | Frequency required. |
| Learning verification modality | The acquisition of all the described skills will be verified by means of an oral examination. Oral test consists of an interview of about 30 minutes aiming to ascertain the knowledge and understanding level acquired by the student on theoretical and methodological contents as indicated on the program. Furthermore, the ability to perform mathematical calculations aimed at performing laboratory procedures will be also evaluated. |
| Extended program | Theoretical lessons: Biodrugs, modifications of natural proteins for the development of biodrugs. New genetic engineering approaches for the design and development of biodrugs: CRISPR/Cas9. Systems to produce recombinant proteins in prokaryotes, insects, yeasts, and eukaryotes. Conventional, retroviral, and viral plasmid vectors. Biodrugs as drug therapy for autoimmune, cardiovascular, inflammatory and tumor diseases treatment. Practical lessons: Snapgene and DNASTAR genomics: Software for biodrugs virtual design. Advanced technologies for biomolecules DNA cloning: Gibson assembly, In-Fusion Cloning. Molecular identification of single nucleotide polymorphisms and site-specific mutagenesis. Cloning, purification and functional analysis of the CTLA-4-Ig fusion protein. |
APPLIED BIOLOGY
| Code | GP003563 |
|---|---|
| Location | PERUGIA |
| CFU | 6 |
| Teacher | Rita Romani |
| Teachers |
|
| Hours |
|
| Learning activities | Caratterizzante |
| Area | Discipline biotecnologiche comuni |
| Academic discipline | BIO/13 |
| Type of study-unit | Obbligatorio (Required) |
| Language of instruction | Italian |
| Contents | Course objectives are: to deepen the knowledge of the structure and cellular functions, genesis of eso vesicles and their role in cell communication and the possible diagnostic and therapeutic applications, depending on the biological niches and finally explore some mechanism of transmission of hereditary characteristics |
| Reference texts | Bruce Alberts, Alexander Johnson, Julian Lewis, Martin Raff, Keith Roberts, Peter Walter "Biologia molecolare della cellula" ZanichelliSome topics covered during the course, for their relevance will have to be supplemented with scientific articles because not yet available in textbooks. |
| Educational objectives | Course objectives are: to deepen the knowledge of the structure and cellular functions, genesis of eso vesicles and their role in cell communication and the possible diagnostic and therapeutic applications, depending on the biological niches and finally explore some mechanism of transmission of hereditary characteristics |
| Prerequisites | To achieve the training objectives, the student requires knowledge of Cell Biology and Molecular Biology. |
| Teaching methods | Practical and theoretical classes |
| Other information | Frequency required |
| Learning verification modality | Oral exam consisting of an about 20-minute discussion aimed at ascertaining the degree of comprehension of the student about theoretical and methodological contents of the program. |
| Extended program | Post synthetic fate of proteins. Vesicular trafficking and vesicle role in cellular communication. Biotechnological applications of eso vesicles. Symmetric and asymmetric cell division. Cellular aging process: cellular senescence, autophagy, repair systems of DNA damage.Stem cells and biological niches.Exceptions to Mendel's laws, genetic imprinting, uniparental disomy genetic mosaicism, chimeras, multifactorial traits. Mitochondrial inheritance. Main techniques applied in medical genetics for the diagnosis Practical exercises: Stem cell differentiation: medium preparation , histochemical stains and PCR in order to demostrate the differentiation. Formation of embryoid bodies. Isolation extracellular vesicles, RNA extraction, PCR |