Unit GENERAL AND APPLIED ENZYMOLOGY
- Course
- Molecular and industrial biotechnology
- Study-unit Code
- GP004114
- Curriculum
- In all curricula
- Teacher
- Brunella Tancini
- Teachers
-
- Brunella Tancini
- Carla Emiliani (Codocenza)
- Hours
- 37 ore - Brunella Tancini
- 10 ore (Codocenza) - Carla Emiliani
- CFU
- 6
- Course Regulation
- Coorte 2022
- Offered
- 2022/23
- Learning activities
- Caratterizzante
- Area
- Discipline biologiche
- Academic discipline
- BIO/10
- Type of study-unit
- Obbligatorio (Required)
- Type of learning activities
- Attività formativa monodisciplinare
- Language of instruction
- Italian
- Contents
- Theoretical lessons: Enzymatic catalysis and examples of catalytic mechanisms. Enzyme kinetics. Mechanisms of inhibition and regulation of enzymatic activity. Methods of measurement of enzymatic activity. Industrial applications of enzymes. Methods of immobilization of enzymes. Use of enzymes in biphasic systems. Biosensors. Diagnostic and therapeutic use of enzymes. Practical training: determination of the kinetic parameters of an enzyme; enzimatic assay of an immobilized enzyme
- Reference texts
- - Nelson, M.M.Cox, Principi di Biochimica di Lehninger, Ed. Zanichelli
- R.Verga, M.S.Pilone: Biochimica industriale; Casa Editrice Sprinter
- Material provided by the teacher - Educational objectives
- The main purpose of the course is to provide students with a good knowledge of the structural and functional characteristics of enzymes and their potential applications in various biotechnological fields. The acquired knowledge must allow the student to understand the different modes of action of enzymes and critically evaluate the parameters that can influence their activity in order to be able to predict the possible applications of enzymes in the various biotechnological sectors, with particular regard to the industrial sector. . The practical exercises will provide the student with the necessary tools to apply the theoretical knowledge acquired in order to solve problems and design experiments.
- Prerequisites
- In order to be able to understand and profitably attend the course it is useful for the student to have a good knowledge of basic elements of general and inorganic chemistry, organic chemistry and biochemistry
- Teaching methods
- The course is organized as follows:
- Lectures on all subjects of the course. Lessons will be integrated with slide projections and movies.
- Practical training: Students will be divided into groups of 3-4 people per group and carry out the essays in the program. The practical exercise will be preceded by an oral presentation. During the training, students will be supported by skillful and qualified personnel.
Virtual Lab: the student will be given credentials to access the program and conduct planned experiments. - Other information
- Tutoring activities during the course and the practical training will be performed.
For the timetable of lessons please consult the website of the course: http://biotecnologie.unipg.it - Learning verification modality
- The exam includes an oral test which consists of a discussion on the topics covered during the course on the basis of the provisions of the program. The duration of the exam may vary depending on the progress of the test itself, with an average duration of about 30-40 minutes. The oral exam aims to assess the level of knowledge and understanding achieved by the student regarding the topics covered. In addition, this interview will allow to verify the student's ability to communicate what has been learned with language properties and clarity of presentation
- Extended program
- Theoretical lessons: Structure and function of enzymes: general principles. Enzyme-substrate interaction models. Enzymatic catalysis and examples of catalytic mechanisms. Models of enzymatic kinetics. Enzyme inhibition. Control mechanisms of enzymatic activity. Methods of measurement of enzymatic activity and factors that influence it. Methods of immobilization of enzymes. Industrial applications of enzymes: general principles. Examples of the use of enzymes in industry. Use of enzymes in biphasic systems. Biosensors. Use of enzymes in diagnostics: biological role and diagnostic significance of some enzymes. Use of enzymes in therapy. Practise. SDS-PAGE-electrophoresis; enzymatic assay of an immobilized enzyme