Unit MOLECULAR TOOLS FOR FAUNISTIC ANALYSIS
- Course
- Natural and environmental sciences and technologies
- Study-unit Code
- 55320706
- Curriculum
- In all curricula
- Teacher
- Livia Lucentini
- Teachers
-
- Livia Lucentini
- Hours
- 42 ore - Livia Lucentini
- CFU
- 6
- Course Regulation
- Coorte 2021
- Offered
- 2022/23
- Learning activities
- Caratterizzante
- Area
- Discipline biologiche
- Academic discipline
- BIO/06
- Type of study-unit
- Obbligatorio (Required)
- Type of learning activities
- Attività formativa monodisciplinare
- Language of instruction
- Italian
- Contents
- Introduction to the use of molecular markers in faunistic management. resolution of taxonomic ambiguity and application of both nuclear and mt molecular markers. National and local examples.
- Reference texts
- Power point and word files provided.
Population Genetics for Animal Conservation. CAMBRIDGE. - Educational objectives
- Comprehension of the use of molecular markers in faunistic management. resolution of taxonomic ambiguity and application of both nuclear and mt molecular markers. National and local examples. Manipulation and comprehension of biomolecular data
- Prerequisites
- nd
- Teaching methods
- Attendance is strongly recommended
- Learning verification modality
- Written (2h) and oral (about 30 ') tests.
Closed-ended questions (20%), open-ended questions (20%) and exercises (60%). For information on support services for students with disabilities and / or SLD, visit the page http://www.unipg.it/disabilita-e-dsa - Extended program
- Introduction to the use of molecular tools in faunistic management. Molecular methodologies as PCR, restriction enzymes, PCR-RFLP, AFLP, microsatellite, DNA Barcoding and SNPs: theory and practical applications. Microsatellite enriched genomic library. Museum historical collections: importance as scientific tool. DNA extraction from conservative and historical samples. Hardy-Weinberg equilibrium. Mutation and migration indexes. Selection and fitness. Inbreeding. Coefficients I, F, F statistics. On-line databases. Resolution of taxonomic ambiguity - practical examples: trout. Molecular evaluation of a species complex: Baetis rhodani complex. Conservative approach to Perdix in repopulation stock.Molecular evidences on Squalus albus. Relationships between AF, environmental data and molecular characterization. DUI. PCR-RFLP as a tool to individuate food mystifications. Practical applications: cladograms, phylograms. Local molecular taxonomy.