Unit CHEMISTRY AND BIOCHEMISTRY I

BIOCHEMISTRY I - MOD. 1

Code	GP001280
CFU	3
Teacher	Ilaria Bellezza
Teachers	Ilaria Bellezza
Hours	37.5 ore - Ilaria Bellezza
Learning activities	Base
Area	Struttura, funzione e metabolismo delle molecole d'interesse biologico
Academic discipline	BIO/10
Type of study-unit	Obbligatorio (Required)
Language of instruction	Italian
Contents	Structural biochemistry: carbohydrates, lipids, nucleotides, nucleic acids, amino acids, proteins. Hemoglobin and myoglobin
Reference texts	I principi di Biochimica del Lehninger - Nelson, Cox, Zanichelli Harper's Biochimica Illustrata - Murray et al - EMSI Biochimica Umana Salvatore Idelson Gnocchi Biochimica Devlin EdiSES Biochimica Berg, Tymoczko, Stryer Zanichelli Biochimica Medica Siliprandi, Tettamanti Piccin
Educational objectives	The main competence will be to have a general knowledge necessary to address the lessons of Module 2.
Prerequisites	The student must have acquired basic knowledge of the main topics of General Chemistry and Organic Chemistry
Teaching methods	The course is organized in:-frontal lessons-theoretical practical lessons
Other information	The attendance is compulsory at least 75% of lessons. Perugia –School of Medicine – Educational and Scientific Center
Learning verification modality	The final exam consists of a written test with multiple choice questions and an oral exam
Extended program	Nucleotides and Nucleic acids: purine and pyrimidine bases, nucleosides, nucleotides, DNA and RNA. Carbohydrates: monosaccharides, disaccharides, polysaccharides. Lipids: Fatty Acids, Triglycerides, Phospholipids and Glycolipids, Cholesterol. Amino acids and proteins: acid base properties. Peptide bonding. Fibrous and globular proteins. Primary, secondary, tertiary and quaternary structure of proteins. Isoelectric point. Biological functions. Structure and function of biological membranes: composition of biological membranes. Structure and properties of membrane lipids. Structure and properties of membrane proteins. Transport systems across the membrane. Chemical mediators, receptors and signal transduction. Generalities on primary and secondary chemical mediators. Structure and properties of some chemical mediators. Properties of receptors and their localisation. Signal transduction across the plasma membrane. Regulation of adenylate cyclase. cGMP and nitric oxide. Phosphoinositide cycle. Regulation of intracellular calcium concentration. Modulation of enzyme activities by phosphorylation-dephosphorylation reactions. Protein kinases. Induction of protein expression.

BIOCHEMISTRY I - MOD. 2

Code	GP001282
CFU	3
Teacher	Davide Chiasserini
Teachers	Davide Chiasserini
Hours	37.5 ore - Davide Chiasserini
Learning activities	Base
Area	Struttura, funzione e metabolismo delle molecole d'interesse biologico
Academic discipline	BIO/10
Type of study-unit	Obbligatorio (Required)
Language of instruction	Italian
Contents	The Module 2 program covers the following topics: Enzymes. Enzyme kinetics, regulation and inhibition. Coenzyme structures, enzyme mechanisms of action. Catabolism and Anabolism. Bioenergetics. High-energy coumpounds. ATP. Respiratory chain and oxidative phosphorylation. Biosignaling.
Reference texts	Biochimica Medica Siliprandi, Tettamanti - Piccin I principi di Biochimica del Lehninger - Nelson, Cox, Zanichelli Harper's Biochimica Illustrata - Murray et al - EMSI Biochimica Umana Salvatore -Idelson Gnocchi Biochimica Devlin- EdiSES Biochimica Berg, Tymoczko, Stryer- Zanichelli
Educational objectives	At the end of the course the student should be in possession of the main knowledge related to Structural Biochemistry, Catabolism and Anabolism and Energy Biochemistry
Prerequisites	The student must have acquired basic knowledge of General Chemistry and Organic Chemistry and Structural Biochemistry
Teaching methods	The course is organized in: frontal and theoretical-practical lessons
Other information	The attendance is compulsory for at least 75% of lessons. Perugia –School of Medicine – Educational and Scientific Center
Learning verification modality	The final exam consists of a written test with multiple choice questions and an oral exam
Extended program	Enzymes: enzyme catalysis. Active site. Specificity of enzymes. Nature of the enzymes and their classification. Constitutive and inducible enzymes. Kinetics of enzymatic reactions. Michaelis-Menten constant and its meaning. Factors affecting the activity of enzymes. Enzyme inhibition. Mechanisms of enzyme inhibition. Regulation of enzyme activity. Notes on clinical enzymology. Vitamins and coenzymes: food sources of vitamins. Vitamin and nutrition. Water-soluble vitamins (C, biotin, niacin, thiamin, pantothenic acid, riboflavin, pyridoxine, folate, B12). Fat-soluble vitamins (A, E, K, D). Choline, carnitine, taurine. Coenzymes. Structure and function of biological membranes: membrane composition. Structure and properties of membrane lipids. Transport systems through the membrane. Structure and function of the Na + / K + ATPase. General aspects of metabolism: catabolism and anabolism. Bioenergetics: the general principles of chemical thermodynamics. Redox potential. High-energy bonds. ATP: structure and role in the transfer of energy. Organization of metabolic pathways. Respiratory chain and oxidative phosphorylation. Inhibitors of the respiratory chain and uncoupling agents. Biosignaling, features of signal transduction, ligand –receptor interaction, G proteins, protein kinase A, regulation of glycogen phosphorylase. Second messengers: cyclic nucleotides, calcium, phosphatidylinositol 4,5-bisphosphate.

CHEMISTRY - MOD. 1

Code	50202503
CFU	3
Teacher	Pier Luigi Orvietani
Teachers	Pier Luigi Orvietani
Hours	37.5 ore - Pier Luigi Orvietani
Learning activities	Base
Area	Struttura, funzione e metabolismo delle molecole d'interesse biologico
Academic discipline	BIO/10
Type of study-unit	Obbligatorio (Required)

CHEMISTRY - MOD. 2

Code	50202002
CFU	2
Teacher	Pier Luigi Orvietani
Teachers	Pier Luigi Orvietani
Hours	25 ore - Pier Luigi Orvietani
Learning activities	Affine/integrativa
Area	Attività formative affini o integrative
Academic discipline	BIO/10
Type of study-unit	Obbligatorio (Required)