Unit MOLECULAR BASIS OF HUMAN DISEASE AND IMMUNOLOGY
- Course
- Medical, veterinary and forensic biotechnological sciences
- Study-unit Code
- A001597
- Curriculum
- In all curricula
- CFU
- 12
- Course Regulation
- Coorte 2022
- Offered
- 2022/23
- Type of study-unit
- Obbligatorio (Required)
- Type of learning activities
- Attività formativa integrata
MOLECULAR BASIS OF HUMAN DISEASES
| Code | A001763 |
|---|---|
| CFU | 6 |
| Teacher | Francesco Grignani |
| Teachers |
|
| Hours |
|
| Learning activities | Caratterizzante |
| Area | Discipline biotecnologiche comuni |
| Academic discipline | MED/04 |
| Type of study-unit | Obbligatorio (Required) |
MOLECULAR IMMUNOLOGY
| Code | A001762 |
|---|---|
| CFU | 6 |
| Teacher | Francesco Grignani |
| Teachers |
|
| Hours |
|
| Learning activities | Caratterizzante |
| Area | Discipline biotecnologiche comuni |
| Academic discipline | MED/04 |
| Type of study-unit | Obbligatorio (Required) |
| Language of instruction | English |
| Contents | Biotechnologies using immunological tools in the study, diagnosis and therapy of human pathology, with a selection of the most advanced immunological, cellular and molecular tools. |
| Reference texts | Janeway's Immunobiology Garland Science Abbas, Lichtman, Pillai: Cellular and Molecular Immunology |
| Educational objectives | Basic knowledge of the immune system and the main immunological approaches in the biotechnological sector. In particular, knowledge of classic and modern polyclonal and monoclonal antibody production strategies; on the diagnostic and therapeutic applications of antibodies in oncology, rheumatology and cardiology; on the fundamental immunochemical and immunofluorescence techniques, including the flow cytometer; on the main applications in research of Immunoprecipitation technologies for the analysis of proteins, RNA, and chromatin. |
| Prerequisites | Cell biology and signaling transduction. Innate and specific immune system cells. Basic mechanisms of immune reactivity. |
| Teaching methods | Lectures, class discussions, visualization and commentary of images and laboratory procedures. Viewing movies. Work in a group. Inverted classes. Peer to peer teaching |
| Learning verification modality | Classroom discussion. Presentation of specific topics and insights and Bibliographic research, as oral exam constituents |
| Extended program | - Basic immunology: innate and acquired immunity. Antigens and antigenic recognition by B and T lymphocytes. The antibody response. I-IV hypersensitivity reactions such as allergies and diagnostic tests. - Structure and function of antibodies (Ab) or immunoglobulins (Ig). - Rearrangement of immunoglobulin and antigenic T receptor genes - Signal transduction of the antigenic receptors B and T. - Protein immunoprecipitation: principles and uses. - Immunological assays and methods based on the antigen-antibody reaction: precipitation (in solution and agarose gel) and agglutination. Some applications in the biomedical diagnostic field: blood typing, bacterial typing, pregnancy test, Coombs test. Nephelometry, Turbidimetry - Polyclonal antibodies: production of an antiserum and its applications. - Monoclonal antibodies (mAb): production of murine, chimeric, humanized, human mAb (using phage display technology, transgenic animals and other technologies). - Applications of mAb in the diagnostic and therapeutic biomedical field. - Mechanisms of therapeutic antibody activity - Bispecific antibodies, BITEs and Single Chain Variable Fragments (scFv) - Chimeric antigenic receptors (CARs) - Antibodies used in anti-inflammatory therapy and in immune pathologies - Antibodies used in cholesterol lowering therapy - Immune response to tumors and tumor immunotherapy. - Signal transduction of growth factors and main types of receptors. - Antibodies active on proliferative and angiogenetic signaling - Antibodies useful for activating anti-tumor immunity (CTLA-4 system, PD-1 PDL, others) - Cellular immunotherapy and CAR-Ts - Immunotherapeutic combinations. - Chromatin structure and epigenetic regulation of gene expression - Chromatin Immunoprecipitation (ChIP) - ChIP Sequencing and ChIP on chip - RNA immunoprecipitation (RIP) - RIP-Sequencing - Direct and indirect immunofluorescence techniques for the identification of surface and intracellular antigens. - Flow cytometry. Its applications in the analysis of the normal and pathological white blood cell phenotype. Elements of cell sorting. - Radio Immunological Assays (RIA, radio immuno-assay) and Immuno-Enzyme Adsorption ELISA (enzyme-linked immunosorbent assay), for the identification and dosage of antigens and antibodies. Some applications in the biomedical diagnostic field - Transplants and transplant rejection: tissue typing and donor-recipient compatibility tests. - Immune response to infections and strategies for vaccine development. |