Unit IN VITRO CULTURE
- Course
- Agricultural and environmental sciences
- Study-unit Code
- 80228606
- Curriculum
- Biotecnologie
- Teacher
- Tiziano Gardi
- Teachers
-
- Tiziano Gardi
- Hours
- 60 ore - Tiziano Gardi
- CFU
- 6
- Course Regulation
- Coorte 2016
- Offered
- 2018/19
- Learning activities
- Caratterizzante
- Area
- Discipline della produzione vegetale
- Sector
- AGR/03
- Type of study-unit
- Obbligatorio (Required)
- Type of learning activities
- Attività formativa monodisciplinare
- Language of instruction
- Italian
- Contents
- Description of In vitro cultures. Regeneration methods. Morphological, physiological and biochemical bases of in vitro morphogenesis. Cellular totipotency and in vitro regeneration. Micropropagation phases. Encapsulation and synthetic seeds. Storage and cryoconservation.
- Reference texts
- - "MICROPROPAGAZIONE DELLE PIANTE ORTOFLOROFRUTTICOLE". G. De Paoli, V. Rossi, A. Scozzoli - EDAGRICOLE-Edizioni Agricole della Calderini s.r.l. - Bologna, 1994.
- "VEGETATIVE PROPAGATION. VITRO CULTURE OF HIGHER PLANTS". Pierik R.L.M. - Martinus Nijhoff Publisher - Dordrecht - The Netherlands, 1987.
- "Mezzi e tecniche per la coltura in vitro dei vegetali - Speciale Micropropagazione: Notiziario tecnico". Edizioni: ERSO Ente per la Ricerca la Sperimentazione la divulgazione on Ortofrutticoltura Floricoltura e sementi. - Educational objectives
- Let the student the techniques of micropropagation and periods of withdrawal of the plant material to be placed in vitro; sterilization procedures and the removal of meristematic apexes under aseptic conditions.
- Prerequisites
- Have supported the Botany exam
- Teaching methods
- face-to-fac and Practical training in laboratory
- Other information
- Recommended frequency
- Learning verification modality
- Oral examination
- Extended program
- Definition and meaning of in vitro culture. Methods of regeneration. Bases morphological, physiological and biochemical in vitro morphogenesis. Regeneration and cell totipotency. Micropropagation and cloning in vitro. Stages of micropropagation. Encapsulation technology. Propagules. Capsule. Synthetic seed. Possible applications of micropropagation and encapsulation technology. Procedures for in vitro conservation of germplasm. Laboratory activities: Handling aseptically growing media, Removal of meristematic apexes, preparation capsules and synthetic seeds with different types of vitro-derived explants.