Unit ADVANCED BREEDING
- Course
- Agricultural and environmental biotechnology
- Study-unit Code
- A002229
- Curriculum
- In all curricula
- Teacher
- Daniele Rosellini
- CFU
- 11
- Course Regulation
- Coorte 2022
- Offered
- 2022/23
- Type of study-unit
- Obbligatorio (Required)
- Type of learning activities
- Attività formativa integrata
ADVANCED ANIMAL BREEDING
| Code | A002231 |
|---|---|
| CFU | 5 |
| Teacher | Emiliano Lasagna |
| Teachers |
|
| Hours |
|
| Learning activities | Caratterizzante |
| Area | Discipline biotecnologiche generali |
| Academic discipline | AGR/17 |
| Type of study-unit | Obbligatorio (Required) |
| Language of instruction | English |
| Contents | Importance of biotechnologies in animal science and differences with plant science. Biotechnologies in animal reproduction and their applications. Molecular biotechnologies and their applications in animal science. |
| Reference texts | Books. G. Sali, Manuale di teriogenologia bovina, Edagricole, 1996.R. Renneberg, Biotechnology for Beginners, Academic press, 2007. |
| Educational objectives | he main aims of the course are to provide the necessary skills to be able to apply the main biotechnology applied to the livestock sector of farm animals. More in detail, the course aims to provide to the students the basic knowledge of the anatomy and physiology of the reproductive apparatus. We will then provide all the knowledge necessary to implement the main reproductive biotechnology practiced in livestock species in consideration of their potential as part of animal breeding. They will be finally addressed the aspects related to the most recent and modern applications of molecular biotechnology practices of selection and genetic improvement. The main skills acquired by the student at the end of the course will be:- laboratory analysis of the semen in breeding animals;- molecular analysis using biological samples from animals;-use of biotechnological tools (reproductive and molecular) for the planning and the implementation of the main strategies for animal breeding. |
| Prerequisites | The students need knowledge of biology, genetics and molecular biology. Background animal husbandry, if not already available, will be provided during the Masters’ course. |
| Teaching methods | The course is organized as follows: -lectures on all subjects of the course; -laboratory exercise of individual practice by students. It will be presented the following topics: macroscopic and microscopic analysis of semen to evaluate the bull fertility. Genetic savage techniques. Practical lesson to the ANABIC genetic station. molecular analysis, PCR based, to detect useful and deleterious genes, for the embryo sexing, and for the food molecular traceability. -educational visits LGS and AVANTEA, Cremona, Italy |
| Other information | Attendance to the lessons. Optional but strongly advised |
| Learning verification modality | Final oral exam. Constant monitoring of learning during classes. Seminars. |
| Extended program | Importance of biotechnologies in animal science and differences with plant science. Animal breeding in livestock systems. Biotechnologies in animal reproduction and their applications. Anatomy and physiology of animal reproduction. Techniques: artificial insemination, oestrous synchronisation, embryo transfer, Multiple ovulation and embryo transfer (MOET), embryo splitting, embryo vitrification, semen sexing, in vitro fertilization (FIVET) ovum pick up, intra cytoplasmatic sperm injection (ICSI), nuclear transfer and cloning, genetic salvage. Applications: generation gap reduction, increasing of genetic progress, increasing of breeding soundness, conservation of biodiversity. Molecular biotechnologies and their applications in animal science. Animal cytogenetic: technology and its applications.Techniques: DNA fingerprinting, PCR based tecniques, microsatellites, SNPs, linkage analysis, gene transfer, real time PCR, etc. Applications: parentage analysis, evolutionary studies, identification of advantageous and deletereous loci, embryo sexing, QTLs identification, Marker Assisted Selection, gene assisted introgression, animal transgenesis, conservation of biodiversity, foods molecular traceability. The SNPs markers: genome wide selection and the candidate genes. gEBV of the young bulls. |
ADVANCED PLANT BREEDING
| Code | A002230 |
|---|---|
| CFU | 6 |
| Teacher | Daniele Rosellini |
| Teachers |
|
| Hours |
|
| Learning activities | Caratterizzante |
| Area | Discipline biotecnologiche generali |
| Academic discipline | AGR/07 |
| Type of study-unit | Obbligatorio (Required) |
| Language of instruction | English |
| Contents | The objective of this unit is to provide to the students a deep knowledge of plant breeding methods and of advanced plant genetic engineering techniques. The topics will be plant reproductive systems, the nature of genetic variation in plant populations, the sources of genetic variation for plant breeding: agricultural genetic resources, intra- and interspecific crosses, , mutagenesis, manipulation of chromosome number, genetic engineering and genome editing. The latter two topics will be integrated by reading recent scientific literature in class. The methods of crop breeding in self-, cross-fertilizing, and in vegetatively propagated species, and of variety conservation will be examined. Online exercises on genetic biotechnology and, a problem solving group exercise will be proposed to the students, who will have to outline a project to solve a practical breeding problem in a relevant crop species. The students will also select a crop species with a case study and wil prepare a brief presentantion for the class. |
| Reference texts | Brown et al. Plant Breeding, 2nd edition, Wiley Blackwell, 2014. Lorenzetti et al. Miglioramento genetico delle piante agrarie. New Business Media, Bologna, 2018. Material provided by the instructor via web (e-learning platform Unistudium): presentations and scientific articles |
| Educational objectives | Knowledge - Plant reproductive systems and nature of genetic variation in plant populations - Methods and techniques of plant genetic engineering - Applications of plant genetic engineering - Genome editing in plants - Plant breeding methods for predominantly self-fertilizing plants - Plant breeding methods for predominantly cross-fertilizing plants - Plant breeding methods for vegetatively propagated plants Abilities Class discussion on recent scientific literature In silico identification of transgenic events Problem solving group exercise: - Choosing the methods of plant breeding depending on plant material and objectives - Critical assessment of the plant breeding toolbox |
| Prerequisites | The student should have a good background in genetics, including population and quantitative genetics, plant biology and botany. |
| Teaching methods | Computer-aided presentations, reading and commenting scientific articles in class, computer lab exercises, field trips |
| Other information | The detailed timetable will be provided the first day of class. Contact: Daniele Rosellini Tel.: 075 5856211, E-mail: daniele.rosellini@unipg |
| Learning verification modality | Final oral examination. Active participation in class and the quality of the problem solving exercise will be taken into account. |
| Extended program | - Plant reproductive systems and nature of genetic variation in plant populations - Sources of genetic variation: plant genetic resources, intra and interspecific crosses, mutagenesis, ploidy change, in vitro culture, plant genetic engineering. - Applications of plant genetic engineering - Genome editing in plants - Plant breeding methods for predominantly self-fertilizing plants - Plant breeding methods for predominantly cross-fertilizing plants - Plant breeding methods for vegetatively propagated plants - Class discussion on recent scientific literature - In silico identification of transgenic events - Problem solving group exercise: choosing the methods of plant breeding depending on plant material and objectives and critical assessment of the plant breeding toolbox |