| Code |
A001701 |
| CFU |
2 |
| Teacher |
Oxana Bereshchenko |
| Teachers |
|
| Hours |
- 25 ore - Oxana Bereshchenko
|
| Learning activities |
Caratterizzante |
| Area |
Medicina di laboratorio e diagnostica integrata |
| Academic discipline |
BIO/12 |
| Type of study-unit |
Obbligatorio (Required) |
| Language of instruction |
Italian |
| Contents |
General principles of the Laboratory Medicine. Basic principles of the laboratory methods and data interpretation. ¿Some basic knowledge of the new techniques used in “personalized medicine” and useful for identification of novel disease markers |
| Reference texts |
G. Federici¿Laboratory medicine.¿McGraw-Hill; ¿Essential clinical biochemistry. E. Albi, T. Beccari, S. Cataldi, Zanichelli;¿Biochimica Clinica e Medicina di Laboratorio, Ciaccio, Lippi, EdiSES |
| Educational objectives |
The objective of this course is to describe general principles of techniques used in a biomedical laboratory and provide tools to evaluate the significance, methods and degree of information the medical personnel can obtain from a standard analytes or from specific disease markers. ¿The course provides to the medical student the overview of biochemistry and molecular biology methods used in the the laboratory |
| Prerequisites |
The course makes numerous connections with physics, chemistry, biochemistry and physiology, herefore basic knowledge related to organic and inorganic chemistry, physics and physiology is required. |
| Teaching methods |
Frontal teaching and ingratiate teaching |
| Learning verification modality |
Written test (when epidemiological situation permits it):¿Multiple choice questions.¿Five possible answers of which only one is correct.¿Exam duration 30 minutes.¿The evaluation criteria will be based on the understanding of the topics covered, on the acquisition of the proposed concepts and methodologies and on the ability to apply them independently and consciously. |
| Extended program |
Laboratory exams. Biochemistry methods and techniques. Sampling. Error in laboratory exams. Analitical and pre-analytical variability. Normalization of the pre-analytical phase. Centrifugation techniques. ¿Biological variability. Reference values. Test accuracy, precision, sensibility, repeatability, reproducibility. Diagnostic sensibility and specificity. Exams of the urines. ¿Spectrophotometry techniques and its applications. Principles of clinical enzymology. Application of clinical enzymology. Markers of organ lesion. Isoenzymes. Electrophoresis. Peptidogram.¿Methods involving antibodies. ELISA. Flow cytometry. Western Blotting.¿Transcription, replication, translation, RNA, DNA, gene, splicing, epigenetica. Alterazioni genetiche. PCR, real-time PCR. Molecular diagnostics. Principles, applications, notions genotype, phenotype, mutation. |
