Unit GENETICS AND GENOMICS

Course
Biology
Study-unit Code
A001058
Curriculum
Biomolecolare
Teacher
Hovirag Lancioni
Teachers
  • Hovirag Lancioni
Hours
  • 47 ore - Hovirag Lancioni
CFU
6
Course Regulation
Coorte 2022
Offered
2022/23
Learning activities
Caratterizzante
Area
Discipline del settore biomolecolare
Academic discipline
BIO/18
Type of study-unit
Obbligatorio (Required)
Type of learning activities
Attività formativa monodisciplinare
Language of instruction
Italian
Contents
Advanced molecular methods for the study of prokaryotic and eukaryotic genomes.
Study of genomic complexity.
Reference texts
Brown TA – Genomes 4. Edises and other didactic material provided by the teacher
Educational objectives
The course aims to broaden the knowledge of Genetics by making students acquire notions and skills of Genetics and Genomics that can be spent in different areas from genomics to evolution, biodiversity, medical genetics, etc. At the end of the course students will have to know how to apply a scientific methodology for understanding the structure and complexity of prokaryotic and eukaryotic genomes, as well as knowing how to participate in group discussion on a research topic.
Prerequisites
Genetics
Teaching methods
Frontal lessons, laboratory exercises and didactic seminars.
Other information

Learning verification modality
Verification will take place through oral examination and through verification of laboratory activities. Critical reading of scientific papers
Extended program
Introduction to genomics: definition and traditional disciplines of genetics integrated into genomics.
Structural and genetic organization of prokaryotic genomes and eukaryotic organelles.
Structural and genetic organization of eukaryotic genomes. The human genome. Development of the Human Genome (HGP) project. Historical notes, cultural and organizational aspects. The main scientific strategies and approaches used for the realization of the HGP and its purposes. Organization of a large-scale sequencing project.
Genetic and molecular techniques that allow the study, molecular characterization and annotation of entire genomes. Massive and ultra-massive sequencing techniques and applications used for the study of genomes, the concepts of assembly and genomic annotation, and the methods of investigation of genomic databases to interpret and undertake genomic studies. Second generation sequencing methods. Third generation sequencing methods. The role of bioinformatics.
Experimental approaches on a genomic scale that can be used to identify genes and genetic pathways.
Methods for studying molecular variability. Metabarcoding and metagenomics. Screening of genomic-scale mutations as a strategy for functional genetic dissection of complex biological processes. Analysis of genomic sequences. Looking for an Open Reading Frame (ORF). Assigning a function to a gene: Computer-based and experimental approaches. Forward and reverse genetics.
Evolutionary models and molecular phylogeny. Methods for the creation of phylogenetic trees: based on the calculation of genetic distances (e.g. UPGMA) or on the analysis of individual sites (e.g. Maximum Parsimony). The molecular clock: from molecular divergence to time estimation. Examples of phylogeographic analysis applied to the study of the origin and evolution of modern human. Genetics of human taste perception
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