Unit BIOCHEMICAL METHODS
- Course
- Biology
- Study-unit Code
- GP004082
- Curriculum
- Biosanitario
- Teacher
- Serena Porcellati
- Teachers
-
- Serena Porcellati
- Sandra Buratta (Codocenza)
- Elisabetta Chiaradia (Codocenza)
- Hours
- 25 ore - Serena Porcellati
- 12 ore (Codocenza) - Sandra Buratta
- 10 ore (Codocenza) - Elisabetta Chiaradia
- CFU
- 6
- Course Regulation
- Coorte 2022
- Offered
- 2022/23
- Learning activities
- Affine/integrativa
- Area
- Attività formative affini o integrative
- Academic discipline
- BIO/10
- Type of study-unit
- Opzionale (Optional)
- Type of learning activities
- Attività formativa monodisciplinare
- Language of instruction
- Italian
- Contents
- Chromatographic techniques.
Enzyme assays - Reference texts
- Keith Wilson and John Walker: Biochimica e biologia molecolare:Principi e tecniche¿ EDs. 2019 Raffaello Cortina Editore. ¿
M.Maccarrone: Metodologie Biochimiche e Biomolecolari, Zanichelli Editore
Bonaccorsi, Contestabile, Di Salvo -Metodologie biochimiche-Casa Editrice Ambrosiana. Distrib. Zanichell - Educational objectives
- Knowledge about the chromatographic methods for the study of the main biomolecules.
- Prerequisites
- Basic knowledge in the field of organic chemistry and biochemistry
- Teaching methods
- Lectures with the help of slides
- Other information
- Optional
- Learning verification modality
- Interview on course topics , within the exam with the other teachers.
- Extended program
- Lab's instruments. Wow to prepare solutions.
Protein extraction and cellular lysis by tissue's omogeneization.
Methods and techniques for protein purification: centrifugation, fractional precipitation of proteins
dialysis.
Chromatographic techniques: introduction.
Molecular exclusion chromatography.
Ion exchange chromatography.
Affinity chromatography.
Partition chromatography:
thin-layer chromatography.
Adsorption chromatography. High-pressure liquid chromatography.
Enzyme kinetics:
Measure of enzyme catalitic activity.
Specific activity, Km and Vmax values.
Enzymatic inhibition: competitive, uncompetitive.