Unit LABORATORY MEDICINE

CLINICAL BIOCHEMISTRY - MOD. 1

Code	GP005711
Location	PERUGIA
CFU	1
Teacher	Paolo Gorello
Teachers	Paolo Gorello
Hours	12 ore - Paolo Gorello
Learning activities	Caratterizzante
Area	Medicina di laboratorio e diagnostica integrata
Sector	BIO/12
Type of study-unit	Obbligatorio (Required)
Language of instruction	Italian
Contents	- Generation of laboratory data and its critical interpretation.- Reliability of test results: precision, accuracy, sensitivity, specificity.- Quality management, predictive value and decision-making levels.- Units of measurement in Clinical Biochemistry, sources of preanalytical variability, reference values, Biological variability.- Principles and techniques for collecting, processing and storage of biological samples.- Methods used in Clinical Biochemistry:Centrifugation techniques, UV-Visible spectrophotometry and Spectrofluorimetry- Point-of-Care testing, Chemical dry state.- Clinical Enzymology.- Biochemical parameters. Markers of function and injury of tissue or organ with particular reference to markers of myocardial damage and markers of liver function.
Reference texts	Slides and notes provided by teacher
Educational objectives	The main aim of this teaching is to provide students with the theoretical principles useful to identify and analyze the sources of variability of laboratory data. The program includes the presentation of some investigation methods of laboratory, their application in the diagnosis of diseases. The main knowledge acquired will be:- Basic knowledge about the collection, processing and storage of biological samples.- Knowledge related to units of measurement in Clinical Biochemistry, sources of preanalytical variability, reference values, biological variability.- Basic concepts of reliability analytical precision, accuracy, sensitivity, specificity.- Basic elements about quality management , predictive value and decision-making levels.- Basic knowledge of Methods used in Clinical Biochemistry:Centrifugation techniques, UV-Visible spectrophotometry and spectrofluorimetry.- Basic knowledge of Clinical Enzymology: enzyme assay, markers of tissue and organ.
Prerequisites	The course requires basic knowledge of Biochemistry, Cell Biology and Genetics.
Teaching methods	Lectures on all subjects of the couse.
Learning verification modality	Written Test.
Extended program	- Generation of laboratory data and its critical interpretation.- Reliability of test results: precision, accuracy, sensitivity, specificity.- Quality management, predictive value and decision-making levels.- Units of measurement in Clinical Biochemistry, sources of preanalytical variability, reference values, Biological variability.- Principles and techniques for collecting, processing and storage of biological samples.- Methods used in Clinical Biochemistry:Centrifugation techniques, UV-Visible spectrophotometry and Spectrofluorimetry- Point-of-Care testing, Chemical dry state.- Clinical Enzymology.- Biochemical parameters. Markers of function and injury of tissue or organ with particular reference to markers of myocardial damage and markers of liver function.

CLINICAL BIOCHEMISTRY - MOD. 2

Code	GP005712
Location	PERUGIA
CFU	1
Teacher	Paolo Gorello
Teachers	Paolo Gorello
Hours	12 ore - Paolo Gorello
Learning activities	Caratterizzante
Area	Medicina di laboratorio e diagnostica integrata
Sector	BIO/12
Type of study-unit	Obbligatorio (Required)
Language of instruction	Italian
Contents	- Description of the most used techniques in Clinical Biochemistry: Nucleic acids electrophoresis, Protein electrophoresis, Western-blotting , ELISA assay, RIA assay, Fluorescence Microscopy.- Basic elements of Molecular Biology (genome, gene, mutations, polymorphisms) and utilization of databases (eg. NCBI, Enseble Genome Browser, Globin Gene Server).- Integration between Clinical Biochemistry methods and Molecular Biology methods : Study of Hemoglobinopathies- Description of the most used techniques in Molecular Diagnostics:end-point PCR , Real-Time PCR, dHPLC, DNA sequencing (Sanger method) , NGS (Next Generation Sequencing).
Reference texts	Comunicated by the professor at the beginning of the course
Educational objectives	The main aim of the teaching is to provide students with the theoretical principles needed to understand the applications and issues that underlie the methods most used in Clinical Biochemistry and Molecular Biology.The main knowledge gained will be:- Basic knowledge of Methods used in Clinical Biochemistry: Nucleic acids and protein electrophoresis, Western-blotting, ELISA, RIA and TestFluorescence microscopy. - Basic knowledge of Methods used in Clinical Molecular Biology: End-point PCR, Real-Time PCR, dHPLC, DNA sequencing using Sanger method,NGS (Next Generation Sequencing). - Basic elements of Molecular Biology (genome, gene, mutations, polymorphisms) and utilization of databases (eg. NCBI, Enseble Genome Browser, Globin Gene Server).
Prerequisites	The course requires basic knowledge of Biochemistry, Cell Biology and Genetics.
Teaching methods	Lectures on all subjects of the couse.
Learning verification modality	Written Test.
Extended program	- Description of the most used techniques in Clinical Biochemistry: Nucleic acids electrophoresis, Protein electrophoresis, Western-blotting , ELISA assay, RIA assay, Fluorescence Microscopy.- Basic elements of Molecular Biology (genome, gene, mutations, polymorphisms) and utilization of databases (eg. NCBI, Enseble Genome Browser, Globin Gene Server).- Integration between Clinical Biochemistry methods and Molecular Biology methods : Study of Hemoglobinopathies- Description of the most used techniques in Molecular Diagnostics:end-point PCR , Real-Time PCR, dHPLC, DNA sequencing (Sanger method) , NGS (Next Generation Sequencing).

CLINICAL MICROBIOLOGY - MOD. 1

Code	GP005710
Location	PERUGIA
CFU	1
Teacher	Antonella Mencacci
Teachers	Antonella Mencacci
Hours	12 ore - Antonella Mencacci
Learning activities	Caratterizzante
Area	Patologia generale e molecolare, immunopatologia, fisiopatologia generale, microbiologia e parassitologia
Sector	MED/07
Type of study-unit	Obbligatorio (Required)
Language of instruction	Italian
Contents	Clinical Microbiology and Laboratory organization (BSL2 and BSL3). Microbiological diagnosis of bacterial, viral, fungal, and parasitic infectious diseases: microscopy, culture, molecular tests, immunodiagnosis and serology. Antibiotic susceptibility testing.
Reference texts	Microbiologia Medica. Murray PR, Rosenthal LS, Pfaller MA. 7th Ed. Edra Masson and lessons' slides
Educational objectives	Knowledge of the microbiologic methods used for the etiologic diagnosis of infections. This is fundamental for studing the second part of the course (clinical microbiology of infectious).
Prerequisites	Knowledge of general microbiology and of fundamentals od immunology
Teaching methods	face-to face lessons and laboratory practice
Learning verification modality	Oral examination
Extended program	Clinical Microbiology and Laboratory organization. Biosafety level 2 and 3 Laboratories. Microbiological diagnosis (direct and serology) of bacterial, viral, fungal, and parasitic infectious diseases. Microscopy, culture, molecular tests, immunodiagnosis and serology. Antibiotic susceptibility testing for Gram positive and Gram negative pathogens. Sintesi del Programma: Clinical Microbiology and Laboratory organization (BSL2 and BSL3). Microbiological diagnosis of bacterial, viral, fungal, and parasitic infectious diseases: microscopy, culture, molecular tests, immunodiagnosis and serology. Antibiotic susceptibility testing.

CLINICAL MICROBIOLOGY - MOD. 2

Code	GP005713
Location	PERUGIA
CFU	1
Teacher	Antonella Mencacci
Teachers	Antonella Mencacci
Hours	12 ore - Antonella Mencacci
Learning activities	Caratterizzante
Area	Patologia generale e molecolare, immunopatologia, fisiopatologia generale, microbiologia e parassitologia
Sector	MED/07
Type of study-unit	Obbligatorio (Required)
Language of instruction	Italian
Contents	Microbiological diagnosis of bacterial, viral, fungal, and parasitic infections.
Reference texts	Microbiologia Medica. Murray PR, Rosenthal LS, Pfaller MA. Settima Edizione. Edra Masson and lessons' slides
Educational objectives	Knowledge of appropriate diagnostic approach to infections and of reading reports.
Prerequisites	Clinical Microbiology mod. 1 and chemical biochemistry
Teaching methods	Face-to-face lessons and laboratory practice
Learning verification modality	Oral examination
Extended program	Microbiological diagnosis of bacterial, viral, fungal, and parasitic infections: bloodstream infections, urinary tract infections, Central nervous system ingections, gastrointestinal infections, respiratory infections, sexually transmitted infections, soft tissues infections.

CLINICAL PATHOLOGY

Code	GP005709
Location	PERUGIA
CFU	2
Teacher	Stefano Brancorsini
Teachers	Stefano Brancorsini
Hours	24 ore - Stefano Brancorsini
Learning activities	Caratterizzante
Area	Medicina di laboratorio e diagnostica integrata
Sector	MED/05
Type of study-unit	Obbligatorio (Required)

PROFESSIONALISING TRAINING IN CLINICAL MICROBIOLOGY

Code	GP005715
Location	PERUGIA
CFU	1
Teacher	Antonella Mencacci
Teachers	Antonella Mencacci
Hours	25 ore - Antonella Mencacci
Learning activities	Altro
Area	Tirocini formativi e di orientamento
Sector	MED/07
Type of study-unit	Obbligatorio (Required)
Language of instruction	Italian
Contents	Sampling of clinical specimens for microbiology. Principal microbiologic diagnostic methods used in bacteriology, viralogy, and mycology.
Reference texts	See Clinical Microbiology - EUCAST
Educational objectives	Knowledge of appropriate tests to diagnose bacterial and viral infections.
Teaching methods	Laboratory practice
Learning verification modality	oral examination
Extended program	Sampling of clinical specimens for microbiology. Upper respiratory tract and genital tract sampling. Wound sampling. Serum sampling. Principal microbiologic diagnostic methods: microscopy, culture, antimicrobial susceptibility testing. Mass spetrometry MALDI-TOF for bacteria identification. Real-time PCR.

PROFESSIONALISING TRAINING IN CLINICAL PATHOLOGY

Code	GP005714
Location	PERUGIA
CFU	1
Teacher	Stefano Brancorsini
Teachers	Stefano Brancorsini
Hours	25 ore - Stefano Brancorsini
Learning activities	Altro
Area	Tirocini formativi e di orientamento
Sector	MED/05
Type of study-unit	Obbligatorio (Required)