Unit GENERAL MICROBIOLOGY
- Course
- Biotechnology
- Study-unit Code
- GP000525
- Curriculum
- In all curricula
- CFU
- 6
- Course Regulation
- Coorte 2019
- Offered
- 2019/20
- Learning activities
- Base
- Area
- Discipline biologiche
- Academic discipline
- BIO/19
- Type of study-unit
- Obbligatorio (Required)
- Type of learning activities
- Attività formativa monodisciplinare
GENERAL MICROBIOLOGY - Canale A
| Code | GP000525 |
|---|---|
| CFU | 6 |
| Teacher | Ermanno Federici |
| Teachers |
|
| Hours |
|
| Learning activities | Base |
| Area | Discipline biologiche |
| Academic discipline | BIO/19 |
| Type of study-unit | Obbligatorio (Required) |
| Language of instruction | Italian |
| Contents | iology of microorganisms. Microbial diversity (eukaryotes, prokaryotes and viruses). The prokaryotic cell (shape, size and arrangements) and its structures (cytoplasm, cell wall, cytoplasmic membrane, endospores, etc). Functional properties of bacteria. Nutrition and growth. Kinetics of cell division. Physical and chemical requirements. Metabolism. Studying bacteria in the laboratory: cultures, isolations and counts. Microscope. Sterilisation The bacterial genome. Variability and gene transfer. Principles of bacterial taxonomy (nomenclature, classification, identification). |
| Reference texts | Brock, Biologia dei microrganismi. Prescott, Microbiologia generale |
| Educational objectives | Expertise in Biotecnology – knowledge of the structure and the biological features of viruses and bacteria, including genetically modified microorganisms; Proficiency in applying knowledge of mathematical, physical and statistical models for the processing of information and experimental data concerning biological processes and systems; Competence in theoretical and practical knowledge of basic microbiological tecniques |
| Prerequisites | biology disciplines (I° semester) |
| Teaching methods | Lessons |
| Other information | Refer to the teacher |
| Learning verification modality | Oral exam Info for disable students http://www.unipg.it/disabilita-e-dsa |
| Extended program | History of Microbiology. Biodiversity and taxonomy: grouping of microrganisms according to Haeckel and Whittaker and evolutionary relationships among living organisms. General Bacteriology. Organization of biological systems. Differences between prokaryotic and eukaryotic organisms. Structure and ultrastructure of the bacterial cell. Size, shape and arrangements. Chemical composition. The circular DNA of bacteria. Cytoplasm and inclusions. Cytoplasmic membrane and its functions. Cell wall (composition, molecular structure, differences between gram-positive and gram-negative). Structures outside the cell wall: cilia, flagella, fimbriae, capsule, glycocalyx, etc.). Protoplasts and spheroplasts. Spores and sporulation. Microscopy and bacteria staining. Bacterial metabolism. Enzymes and their regulation (constitutive enzymes, inducible and repressible). Energy processes. Fermentation, aerobic and anaerobic respiration. Comparisons of energy yields. Nutrition. Nutritional types: classification according to the nutrients, the energy source of carbon. Essential metabolites and growth factors. Bacterial multiplication and factors affecting it. Growth cycle. Growth parameters. Synchronous cultures. Continuous culture. Diauxic growth. Pathogenicity and virulence (infection and infectious disease). Virulence factors. Variability of virulence factors. Bacterial toxins. Antibiotic resistance. Sterilization. Theory and practical methodologies. Sterility controls. Genotype and phenotype. Physiological adaptation. Clonal selection. Alteration of phenotype. Genotypic variations. Bacterial mutants. Plasmids. Episomi. F and R factors. Recombination phenomena: transformation, transduction, conjugation. |
GENERAL MICROBIOLOGY - Canale B
| Code | GP000525 |
|---|---|
| CFU | 6 |
| Teacher | Ermanno Federici |
| Teachers |
|
| Hours |
|
| Learning activities | Base |
| Area | Discipline biologiche |
| Academic discipline | BIO/19 |
| Type of study-unit | Obbligatorio (Required) |
| Language of instruction | Italian |
| Contents | iology of microorganisms. Microbial diversity (eukaryotes, prokaryotes and viruses). The prokaryotic cell (shape, size and arrangements) and its structures (cytoplasm, cell wall, cytoplasmic membrane, endospores, etc). Functional properties of bacteria. Nutrition and growth. Kinetics of cell division. Physical and chemical requirements. Metabolism. Studying bacteria in the laboratory: cultures, isolations and counts. Microscope. Sterilisation The bacterial genome. Variability and gene transfer. Principles of bacterial taxonomy (nomenclature, classification, identification). |
| Reference texts | Brock, Biologia dei microrganismi. Prescott, Microbiologia generale |
| Educational objectives | Expertise in Biotecnology – knowledge of the structure and the biological features of viruses and bacteria, including genetically modified microorganisms; Proficiency in applying knowledge of mathematical, physical and statistical models for the processing of information and experimental data concerning biological processes and systems; Competence in theoretical and practical knowledge of basic microbiological tecniques |
| Prerequisites | biology disciplines (I° semester) |
| Teaching methods | Lessons |
| Other information | Refer to the teacher |
| Learning verification modality | Oral exam Info for disable students http://www.unipg.it/disabilita-e-dsa |
| Extended program | History of Microbiology. Biodiversity and taxonomy: grouping of microrganisms according to Haeckel and Whittaker and evolutionary relationships among living organisms. General Bacteriology. Organization of biological systems. Differences between prokaryotic and eukaryotic organisms. Structure and ultrastructure of the bacterial cell. Size, shape and arrangements. Chemical composition. The circular DNA of bacteria. Cytoplasm and inclusions. Cytoplasmic membrane and its functions. Cell wall (composition, molecular structure, differences between gram-positive and gram-negative). Structures outside the cell wall: cilia, flagella, fimbriae, capsule, glycocalyx, etc.). Protoplasts and spheroplasts. Spores and sporulation. Microscopy and bacteria staining. Bacterial metabolism. Enzymes and their regulation (constitutive enzymes, inducible and repressible). Energy processes. Fermentation, aerobic and anaerobic respiration. Comparisons of energy yields. Nutrition. Nutritional types: classification according to the nutrients, the energy source of carbon. Essential metabolites and growth factors. Bacterial multiplication and factors affecting it. Growth cycle. Growth parameters. Synchronous cultures. Continuous culture. Diauxic growth. Pathogenicity and virulence (infection and infectious disease). Virulence factors. Variability of virulence factors. Bacterial toxins. Antibiotic resistance. Sterilization. Theory and practical methodologies. Sterility controls. Genotype and phenotype. Physiological adaptation. Clonal selection. Alteration of phenotype. Genotypic variations. Bacterial mutants. Plasmids. Episomi. F and R factors. Recombination phenomena: transformation, transduction, conjugation. |