Unit DIAGNOSTIC METHODS IN PATHOLOGICAL ANATOMY
- Course
- Biomedical laboratory techniques
- Study-unit Code
- 50999008
- Curriculum
- In all curricula
- Teacher
- Angelo Sidoni
- CFU
- 8
- Course Regulation
- Coorte 2020
- Offered
- 2021/22
- Type of study-unit
- Obbligatorio (Required)
- Type of learning activities
- Attività formativa integrata
MOLECOLAR BIOLOGY'S METHODS AND THECNIQUES IN PATHOLOGICAL ANATOMY
Code | 50993201 |
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CFU | 1 |
Teacher | Martina Mandarano |
Teachers |
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Hours |
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Learning activities | Affine/integrativa |
Area | Attività formative affini o integrative |
Academic discipline | MED/46 |
Type of study-unit | Obbligatorio (Required) |
Contents | Principles and protocols of the different techniques for DNA analysis in tissue samples for pathological anatomy |
Educational objectives | Application of molecular techniques in histological and cytological samples |
HYSTOPATHOLOGI'S NOTIONS AND ELEMENTS OF AUTOPSY
Code | 50666802 |
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CFU | 2 |
Teacher | Angelo Sidoni |
Teachers |
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Hours |
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Learning activities | Caratterizzante |
Area | Scienze e tecniche di laboratorio biomedico |
Academic discipline | MED/08 |
Type of study-unit | Obbligatorio (Required) |
Contents | Basic histopathological aspects of acute and chronic inflammations, cell degenerations, disorders of cell growth, differentiation and maturation. Dissection protocols and organ removal modalities during autopsy. |
Educational objectives | The student should acquire competence in the main activity of the histopathology laboratory |
ORGANIZATION OF ANATOMIC PATHOLOGY LABORATORY
Code | 50787801 |
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CFU | 1 |
Teacher | Giamprimo Bartoli |
Teachers |
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Hours |
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Learning activities | Caratterizzante |
Area | Scienze e tecniche di laboratorio biomedico |
Academic discipline | MED/46 |
Type of study-unit | Obbligatorio (Required) |
Language of instruction | Italian |
Contents | Organizzazione del Laboratorio di Isopatologia, competenze e responsabilità del TLB che opera nell'ambito dell'Anatomia e Istologia patologica |
Reference texts | J.D. Bancroft M. Gamble, Theory and Practice of Histological Techniques, Churchill Livingstone Elservier, Sixth Edition - L. Roco A. Scarpa, Anatomia Patologica Le Basi, UTET. |
Educational objectives | Attraverso il complesso delle lezioni teoriche e pratiche lo studente potrà acquisire le conoscenze relative alla organizzazione e alle competenze necessarie ad affrontare con responsabilità e piena autonomia professionale le attività previste in un moderno laboratorio di Anatomia ed Istologia Patologica. Lo studente: - analizzerà i principali processi tecnici per lo sviluppo della Diagnosi Istopatologica, dovrà acquisire conoscenza delle linee guida, procedure, istruzioni operative, controllo qualità e norme di sicurezza che caratterizzano il laboratorio di Anatomia e Istologia Patologica. |
Prerequisites | Nozioni e fondamenti derivanti dallo studio propedeudico delle metodiche e tecnihe di istologia |
Teaching methods | Il corso prevede lezioni frontali ed è organizzato con forte impronta pratica. Gli argomenti trattati con confronti pratici da affrontare direttamente nel laboratorio. |
Learning verification modality | L'esame orale verrà sostenuto collegialmente da tutti i docenti afferenti al corso di "Metodologie diagnostiche di Anatomia Patologica. |
Extended program | Modalità di invio, trasporto e conservazione dei campioni al laboratorio di Anatomia e istologia Patologica. Preparazione dell'esame istologico su preparati inclusi in paraffina e su preparati sezionati al criostato. Corretta esecuzione di campioni per riduzione da pezzo operatorio. Orientamento dei campioni all'inclusione in paraffina e allestimento dei preparati. Trattamento di particolari campioni "critici": piccole biopsie, agoaspirati, biopsie cutane. Controllo qualità e sicurezza. |
PATHOLOGICAL ANATOMY AND HYSTOLOGY'S TECNIQUES
Code | 50657002 |
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CFU | 2 |
Teacher | Guido Bellezza |
Teachers |
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Hours |
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Learning activities | Caratterizzante |
Area | Scienze e tecniche di laboratorio biomedico |
Academic discipline | MED/08 |
Type of study-unit | Obbligatorio (Required) |
Language of instruction | Italian |
Contents | Main histological, histochemical and immunohistochemical techniques. Quality control of immunohistochemistry. |
Reference texts | "Anatomia Patologica. Le basi." L. Ruco; A. Scarpa UTET Edizioni Scienze Mediche, "Theory and Practice of Histological Techniques" 5th edizione, JD Bancroft; M. Gamble, Churchill Livingstone |
Educational objectives | Knowledge of managing histopathological laboratory. Main histological, histochemical and immunohistochemical techniques. |
Teaching methods | face-to-face - Practical training - Theoretical lessons and practical training - Laboratory |
Learning verification modality | Oral examination |
Extended program | Histochemical stains: connective tissues and stains; demonstration of mucins, lipids and nucleic acids, pigments and minerals. Immunohistochemical techniques. Theory. Mono and polyclonal antibodies. Labels. Immunohistochemical methods: traditional direct technique and indirect technique. Avidin-biotin technique. PAP and APAAP. Polymer. Immunoperoxidase and immunophosphates techniques. Antigen retrieval techniques. Blocking endogenous enzymes. Quality control of immunohistochemistry. |
CYTOPHATOLOGY'S NOYIONS AND TECHNIQUES
Code | 50667302 |
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CFU | 2 |
Teacher | Rachele Del Sordo |
Teachers |
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Hours |
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Learning activities | Caratterizzante |
Area | Scienze e tecniche di laboratorio biomedico |
Academic discipline | MED/08 |
Type of study-unit | Obbligatorio (Required) |
Language of instruction | Italian |
Contents | Methods for collection, retention, fixation of cytological specimens. Processing and staining techniques. |
Reference texts | 1) "Anatomia Patologica. Le basi." L. Ruco; A. Scarpa UTET Edizioni Scienze Mediche 2) "Theory and Practice of Histological Techniques" 5th edizione JD Bancroft; M. Gamble Churchill Livingstone |
Educational objectives | Known how to send the cytologic material, the various techniques of processing of the material and stains. The main skill will: obtain a optimal cytologic slide for an accurate diagnosis. |
Teaching methods | Lessons face-to-face - Practical training at cytology laboratory |
Learning verification modality | Oral questions |
Extended program | Historical overview. Diagnostic cytology. Cytologic sampling techniques: exfoliative cytology, abrasive cytology, aspiration cytology. Advantages and disadvantages respect to histopathology. The role of technician in cytopathology laboratory. Treatment and secure of cytological samples. Fixation, processing and staining techniques. The Papanicolaou stain. Other stains useful in diagnostic cytology. Mounting the cell sample. Immunocytology. Cell-block Diagnostic cytology of female genital tract. The normal female genital tract. Benign disorders and neoplastic lesions. PAP test. Its role in screening programs. |