Unit ADE - Microscopic examination of cells and tissues: techniques and methods
- Course
- Veterinary medicine
- Study-unit Code
- A000671
- Curriculum
- In all curricula
- Teacher
- Luisa Pascucci
- Teachers
-
- Luisa Pascucci
- Hours
- 25 ore - Luisa Pascucci
- CFU
- 1
- Course Regulation
- Coorte 2017
- Offered
- 2021/22
- Learning activities
- A scelta dello studente
- Area
- A scelta dello studente
- Academic discipline
- VET/01
- Type of study-unit
- Opzionale (Optional)
- Type of learning activities
- Attività formativa monodisciplinare
- Language of instruction
- Italian
- Contents
- 1. Behavioral rules in a laboratory of histology: personal and
environmental security management.
2. Histological techniques: preparation of cell and tissue specimens for
cytomorphological/histomorpological, cyto/histochemical, immuno
cyto/histochemical analyses. Principles of immunofluorescence.
3. Principles of electron microscopy: preparation and observation of
samples for electron microscopy.
4. Morphological study of cultured cells. How to work in a cell culture
laboratory: staff flow, behavioral rules, cleanliness and sterility
management. Cellular isolation and cell culture manipulation. Preparation
of cells for microscopic observation. - Reference texts
- Microscopie in biologia e medicina
Cesare Scala,Gianandrea Pasquinelli,Giovanna Cenacchi
Editore: CLUEB - Educational objectives
- D1. KNOWLEDGE AND UNDERSTANDING
At the end of the course, the student will know how to process cytological and histological samples for microscopic observation.
D2. ABILITY TO APPLY KNOWLEDGE AND UNDERSTANDING
At the end of the course, the student must be able to apply the acquired knowledge to the preparation of specimens for microscopic oservation.
D3. AUTONOMY OF JUDGMENT
The student must be able to use the information provided by the teacher in a critical and autonomous way and to apply it to different contexts.
D4. COMMUNICATION SKILLS
At the end of the course, the student must be able to organize and present the acquired knowledge, supporting it with completeness and adequate language.
D5. LEARNING ABILITY
At the end of the training activity, the student must be able to correlate, integrate and manage the acquired knowledge in different specific areas and to increase them in order to apply to a variety of contexts, including research.
t the end of the course, the student will know how to process cytological
and histological specimens for microscopic observation and will be able
to apply this knowledges to specimen preparation. - Prerequisites
- None
- Teaching methods
- The course is exclusively practical so the lessons will be held in the
laboratory and will cover the topics detailed in the program. - Other information
- Learning verification modality
- Checking the correct performance of the practical activities carried out during the course
DSA students are invited to
visit the followung web page http://www.unipg.it/disabilita-e-dsa - Extended program
- 1. Histological techniques:
- behavioral rules in a histology laboratory; personal and environmental
safety management (1h);
- preparation of cell and tissue specimens for cyto/histomorphological,
cyto/histochemcal, immuno cyto/histochemical analysis (8h);
- principles of immunofluorescence (3h).
Modalità di verifica
dell'apprendimento
Orale
Per informazioni sui servizi di supporto agli studenti con disabilità e/o DSA
visita la pagina http://www.unipg.it/disabilita-e-dsa
Programma esteso 1. Tecniche di studio in istologia:
- norme comportamentali in un laboratorio di istologia: gestione della
sicurezza personale e ambientale (1h);
- allestimento di campioni cellulari e tissutali per analisi
cito/istomorfologiche cito/istochimiche immuno-cito/istochimiche (8h);
- Principi di immunofluorescenza (3h).
2. Principi di microscopia elettronica:
- allestimento di preparati per l'osservazione al microscopio elettronico
(5h).
- Osservazione di preparati al microscopio elettronico a trasmissione
(TEM) e/o a scansione (SEM) (2h)
3. Studio morfologico di cellule in coltura:
- come si lavora in un laboratorio di colture cellulari; flusso di personale,
norme comportamentali, pulizia e gestione della sterilità (1h);
- isolamento cellulare e manipolazione di colture cellulari (3h);
- preparazione di cellule per l’osservazione al microscopio (2h).
2. Principles of Electronic Microscopy:
- preparation of samples for electron microscopy (5h);
- observation at Trnsmission (TEM) and /or scanning (SEM) electron
microscope (2h);
3. Morphological study of cultured cells:
- how to work in a cell culture lab; staff flow, behavioral rules, sterility
cleanliness and management (1h);
- cell isolation and cell culture manipulation (3h).
- preparation of cells for microscopic observation (2h).