Unit MEDIAL BIOCHEMISTRY
- Course
- Pharmacy
- Study-unit Code
- 50171305
- Location
- PERUGIA
- Curriculum
- In all curricula
- Teacher
- Carmela Conte
- Teachers
-
- Carmela Conte
- Carmela Conte
- Hours
- 42 ore - Carmela Conte
- 6 ore - Carmela Conte
- CFU
- 6
- Course Regulation
- Coorte 2017
- Offered
- 2019/20
- Learning activities
- Caratterizzante
- Area
- Discipline biologiche e farmacologiche
- Academic discipline
- BIO/10
- Type of study-unit
- Obbligatorio (Required)
- Type of learning activities
- Attività formativa monodisciplinare
- Language of instruction
- Italian
- Contents
- Biochemical bases of hormonal action. Molecular bases of metabolic diseases. Methods of execution and biomedical meaning of the main biochemical clinical analyzes.
- Reference texts
- - Patti, Contestabile e Di Salvo. Metodologie Biochimiche di
Casa Editrice Ambrosiana
- Italo Antonozzi e Elio Gulletta
Medicina di Laboratorio
Piccin
- Giorgio Federici
Medicina di Laboratorio
Mc Graw-Hill - Educational objectives
At the end of the study the student will be able to acquire the basic knowledge of the fundamental principles of biochemical methodologies in the biomedical field.- Prerequisites
- The course aims to address issues concerning the Applied Medical Biochemistry that the students will be able to face after acquisition of the basic knowledge of General and Molecular Biochemistry. It is essential that the student have acquired the knowledges of structure and function of biological macromolecules and basic mechanisms underlying many processes of cell regulation
- Teaching methods
- The course is organized as follows: Lectures on all subjects of the course
- Other information
On unanimous request by the students it is possible perform tests during the course.- Learning verification modality
- The test requires a single oral exam during which the student will answer questions concerning issues of the lectures and listed in the program on the teaching. The proof test includes several questions with open answer. During the interview the student will demonstrate consistency with the question referred, clarity and accuracy of scientific terminology, and ability to transfer knowledge even through graphic representations.
- Extended program
- • Introduction to applied biochemistry
• Biological samples
• Blood: separation of the fluid portion from the corpuscular portion: Hemochromocytometric examination. Red cell parameters, leukocytes and platelets
• Vitamin K and coagulation factors: prothrombin time, thromboplastin and fibrinogen
Urine: Microbiological examination (urine culture)
Standard exam: Physical examination; Chemical examination; Microscopic examination
Specific dosages (of proteins, drugs, etc.);
Plasma protein: determination methods
• Spectroscopic and fluorimetric techniques: applications in the medical field
• Study of proteins and nucleic acids: electrophoresis of plasma proteins.
• Determination of enzymatic activity and importance in diagnostics: the activities of GOT or AST, GPT or ALT enzymes, ALP (alkaline phosphatase), gamma-GT (gamma-glutamyl transferase)
• Determination of iron, ferritin and transferrin
• Immunoglobulins and immunochemical techniques and enzyme immunoassays.
• Radioisotopic and radioimmunological techniques (RIA)
• Insulin, glucagon and blood glucose regulation: Determination of glycaemia and glycosylated Hb
• Plasma lipids and lipoproteins and cardiovascular risk. Determination of total cholesterol, HDL, LDL. Triglyceride determination
• Vitamin profile
• Feces examination: determination of occult blood
•Markers of cardiovascular risk. Determination of C reactive protein, homocysteine
•Markers of heart damage
•Tumor markers
•Markers of oxidative damage
•Recombinant DNA technology in the biomedical field: PCR and therapeutic proteins (production and purification of recombinant proteins)
•Production and purification of polyclonal and monoclonal antibodies.