Unit BIOCHEMISTRY OF CELLULAR COMMUNICATION; PROTEOMICS AND METABOLOMICS
- Course
- Biotechnology
- Study-unit Code
- A000407
- Curriculum
- In all curricula
- Teacher
- Sandra Buratta
- Teachers
-
- Sandra Buratta
- Hours
- 47 ore - Sandra Buratta
- CFU
- 6
- Course Regulation
- Coorte 2020
- Offered
- 2022/23
- Learning activities
- Caratterizzante
- Area
- Discipline biotecnologiche comuni
- Academic discipline
- BIO/10
- Type of study-unit
- Obbligatorio (Required)
- Type of learning activities
- Attività formativa monodisciplinare
- Language of instruction
- Italian
- Contents
- Biochemistry of cell communications: Molecular basis of cell signaling;
Biochemistry of hormones and integration of metabolism in mammals; Molecular mechanisms underlying cell cycle regulation and apoptosis; Biochemistry of cancer cells
Proteomics and metabolomics: Analytical and preparative techniques used in proteomic and metabolomic studies. - Reference texts
- Lessons slides will be available to students as PDF files in UniStudium platform
“Biochimica Medica - Strutturale, metabolica e funzionale” G. Tettamanti (Piccin)
"I principi di Biochimica di Lenhinger"-Zanichelli
“Biochimica con aspetti clinici” Devlin TM (EdiSES)
“Metodologie Biochimiche” Maria Carmela Bonaccorsi, Roberto Contestabile, Martino Di Salvo (Zanichelli) - Educational objectives
- The aim of the course is the study of the molecular and biochemical processes underlying the main cellular processes with particular attention to molecular and biochemical aspects of cell signaling, modification and degradation of proteins, vesicle trafficking and sorting of proteins.
Further, this course aims to provide more detailed and current knowledge about experimental approaches used in proteomics and in metabolomics. The composition of the proteome and the metabolome are important to understand the molecular mechanisms underlying cellular functions and their alterations during physiopatological processes. - Prerequisites
- Basic knowledge in the field of Biochemistry and cell biology
- Teaching methods
- Lectures on all the subjects of the course using slides and movies. Some lessons could be dedicated to the revision of issues proposed by students. Practical lessons will be organized in the laboratory of Biochemistry equipped to carry out proteomic and methabolomic analysis
- Other information
- Attending classes: recommended but not compulsory
- Learning verification modality
- The exam consists of a written test and an oral examination The written test consists in thirty multiple-choice questions regarding the main topics of the lessons (Libreol). Students who achieve a score of at least 18/30 will be admitted to the oral exam. During the oral exam, the student's ability to link and integrate knowledge will be evaluated.
- Extended program
- Biochemistry of cellular communication:
Molecular bases of cell signaling
- Principles of cell signaling
- Intercellular signaling
- Intracellular signaling
- Intracellular receptors, ligand-gated ion channels, G protein-coupled receptors, receptors with enzymatic activity
- Signaling mediated by proteolytic processes
- Phospholipid signal transduction systems
- Connections and integrations between the main signaling cascades
Biochemical and metabolic correlations between tissues during different nutritional and hormonal states
Molecular mechanisms underlying cell cycle regulation and apoptosis; Biochemistry of cancer cells
Proteomic and Metabolomic
Strategies for Protein Purification: Preparative and analytical techniques. Purification methods based on the protein solubility. Dialysis.
Chromatography: Principles and applications of chromatography; chromatografic tecniques: molecular exclusion chromatography, hydrophobic interaction chromatography, reverse phase chromatography, ion exchange chromatography, affinity chromatography. HPLC and FPLC systems. Gas chromatography.
Electrophoresis: General principle. Polyacrylamide gel electrophoresis (PAGE): SDS-PAGE, Isoelectric focusing (IEF), Two dimensional electrophoresis (2DE). Capillary electrophoresis (CE). Western blotting.
Proteomics: Principles and application of proteomic. Gel-based approach: Preparation of samples; Staining methods for identification of proteins separated on gels. 2D difference electrophoresis (DIGE). Image analysis of the 2DE and spot processing.
Mass spectrometry: Introduction to mass spectrometry. ESI and MALDI ionization techniques. MALDI-TOF mass spectrometry and Peptide Mass Fingerprinting. Protein identification by Tandem mass spectrometry.
Metabolomics: General concepts of metabolomic analysis. Analytical technologies used in metabolomics. Examples of clinical metabolomics.
Proteomic and Metabolomic
Strategies for Protein Purification: Preparative and analytical techniques. Purification methods based on the protein solubility. Dialysis.
Chromatography: Principles and applications of chromatography; chromatografic tecniques: molecular exclusion chromatography, hydrophobic interaction chromatography, reverse phase chromatography, ion exchange chromatography, affinity chromatography. HPLC and FPLC systems. Gas chromatography.
Electrophoresis: General principle. Polyacrylamide gel electrophoresis (PAGE): SDS-PAGE, Isoelectric focusing (IEF), Two dimensional electrophoresis (2DE). Capillary electrophoresis (CE). Western blotting.
Proteomics: Principles and application of proteomic. Gel-based approach: Preparation of samples; Staining methods for identification of proteins separated on gels. 2D difference electrophoresis (DIGE). Image analysis of the 2DE and spot processing.
Mass spectrometry: Introduction to mass spectrometry. ESI and MALDI ionization techniques. MALDI-TOF mass spectrometry and Peptide Mass Fingerprinting. Protein identification by Tandem mass spectrometry.
Metabolomics: General concepts of metabolomic analysis. Analytical technologies used in metabolomics. Examples of clinical metabolomics.