Unit LABORATORY MEDICINE

CLINICAL BIOCHEMISTRY - MOD. 1

Code	GP005711
Location	PERUGIA
CFU	1
Teacher	Paolo Gorello
Teachers	Paolo Gorello
Hours	12 ore - Paolo Gorello
Learning activities	Caratterizzante
Area	Medicina di laboratorio e diagnostica integrata
Academic discipline	BIO/12
Type of study-unit	Obbligatorio (Required)
Language of instruction	Italian
Contents	- Generation of laboratory data and its critical interpretation.- Reliability of test results:  precision, accuracy, sensitivity, specificity.- Quality management, predictive value and decision-making levels.- Units of measurement in Clinical Biochemistry, sources of preanalytical variability, reference values, Biological variability.- Principles and techniques for collecting, processing and storage of biological samples.-  Methods used in Clinical Biochemistry:Centrifugation techniques, UV-Visible spectrophotometry and Spectrofluorimetry- Point-of-Care testing, Chemical dry state.- Clinical Enzymology.- Biochemical parameters. Markers of function and injury of tissue or organ with particular reference to markers of myocardial damage and markers of liver function.
Reference texts	Slides and notes provided by teacher
Educational objectives	The main aim of this teaching is to provide students with the theoretical principles useful to identify and analyze the sources of variability of laboratory data. The program includes the presentation of some investigation methods of laboratory, their application in the diagnosis of diseases. The main knowledge acquired will be:- Basic knowledge about the collection, processing and storage of biological samples.- Knowledge related to units of measurement in Clinical Biochemistry, sources of preanalytical variability, reference values, biological variability.- Basic concepts of reliability analytical precision, accuracy, sensitivity, specificity.- Basic elements about quality management , predictive value and decision-making levels.- Basic knowledge of Methods used in Clinical Biochemistry:Centrifugation techniques, UV-Visible spectrophotometry and spectrofluorimetry.- Basic knowledge of Clinical Enzymology: enzyme assay, markers of tissue and organ.
Prerequisites	The course requires basic knowledge of Biochemistry, Cell Biology and Genetics.
Teaching methods	Lectures on all subjects of the couse.
Learning verification modality	Written Test.
Extended program	- Generation of laboratory data and its critical interpretation.- Reliability of test results:  precision, accuracy, sensitivity, specificity.- Quality management, predictive value and decision-making levels.- Units of measurement in Clinical Biochemistry, sources of preanalytical variability, reference values, Biological variability.- Principles and techniques for collecting, processing and storage of biological samples.-  Methods used in Clinical Biochemistry:Centrifugation techniques, UV-Visible spectrophotometry and Spectrofluorimetry- Point-of-Care testing, Chemical dry state.- Clinical Enzymology.- Biochemical parameters. Markers of function and injury of tissue or organ with particular reference to markers of myocardial damage and markers of liver function.

CLINICAL BIOCHEMISTRY - MOD. 2

Code	GP005712
Location	PERUGIA
CFU	1
Teacher	Paolo Gorello
Teachers	Paolo Gorello
Hours	12 ore - Paolo Gorello
Learning activities	Caratterizzante
Area	Medicina di laboratorio e diagnostica integrata
Academic discipline	BIO/12
Type of study-unit	Obbligatorio (Required)
Language of instruction	Italian
Contents	- Description of  the most used techniques in Clinical Biochemistry:  Nucleic acids electrophoresis, Protein electrophoresis,  Western-blotting , ELISA assay, RIA assay, Fluorescence Microscopy.- Basic elements of Molecular Biology (genome, gene, mutations, polymorphisms) and utilization of databases (eg. NCBI, Enseble Genome Browser, Globin Gene Server).- Integration between Clinical Biochemistry methods and Molecular Biology methods : Study of Hemoglobinopathies- Description of  the most used techniques in Molecular Diagnostics:end-point PCR , Real-Time PCR, dHPLC, DNA sequencing (Sanger method) , NGS (Next Generation Sequencing).
Reference texts	Comunicated by the professor at the beginning of the course
Educational objectives	The main aim of the teaching is to provide students with the theoretical principles needed to understand the applications and issues that underlie the methods most used in Clinical Biochemistry and Molecular Biology.The main knowledge gained will be:- Basic knowledge of Methods used in Clinical Biochemistry:  Nucleic acids and protein electrophoresis, Western-blotting, ELISA, RIA and TestFluorescence microscopy. - Basic knowledge of Methods used in Clinical Molecular Biology:   End-point PCR, Real-Time PCR, dHPLC, DNA sequencing using Sanger method,NGS (Next Generation Sequencing). - Basic elements of Molecular Biology (genome, gene, mutations, polymorphisms) and utilization of databases (eg. NCBI, Enseble Genome Browser, Globin Gene Server).
Prerequisites	The course requires basic knowledge of Biochemistry, Cell Biology and Genetics.
Teaching methods	Lectures on all subjects of the couse.
Learning verification modality	Written Test.
Extended program	- Description of  the most used techniques in Clinical Biochemistry:  Nucleic acids electrophoresis, Protein electrophoresis,  Western-blotting , ELISA assay, RIA assay, Fluorescence Microscopy.- Basic elements of Molecular Biology (genome, gene, mutations, polymorphisms) and utilization of databases (eg. NCBI, Enseble Genome Browser, Globin Gene Server).- Integration between Clinical Biochemistry methods and Molecular Biology methods : Study of Hemoglobinopathies- Description of  the most used techniques in Molecular Diagnostics:end-point PCR , Real-Time PCR, dHPLC, DNA sequencing (Sanger method) , NGS (Next Generation Sequencing).

CLINICAL MICROBIOLOGY - MOD. 1

Code	GP005710
Location	PERUGIA
CFU	1
Teacher	Antonella Mencacci
Teachers	Antonella Mencacci
Hours	12 ore - Antonella Mencacci
Learning activities	Caratterizzante
Area	Patologia generale e molecolare, immunopatologia, fisiopatologia generale, microbiologia e parassitologia
Academic discipline	MED/07
Type of study-unit	Obbligatorio (Required)
Language of instruction	Italian

CLINICAL MICROBIOLOGY - MOD. 2

Code	GP005713
Location	PERUGIA
CFU	1
Teacher	Antonella Mencacci
Teachers	Antonella Mencacci
Hours	12 ore - Antonella Mencacci
Learning activities	Caratterizzante
Area	Patologia generale e molecolare, immunopatologia, fisiopatologia generale, microbiologia e parassitologia
Academic discipline	MED/07
Type of study-unit	Obbligatorio (Required)
Language of instruction	Italian
Educational objectives	Knowledge of clinical microbiology of infections.

CLINICAL PATHOLOGY

Code	GP005709
Location	PERUGIA
CFU	2
Teacher	Stefano Brancorsini
Teachers	Stefano Brancorsini
Hours	24 ore - Stefano Brancorsini
Learning activities	Caratterizzante
Area	Medicina di laboratorio e diagnostica integrata
Academic discipline	MED/05
Type of study-unit	Obbligatorio (Required)
Language of instruction	Italian
Contents	Variability in laboratory medicine. Hemochromocytometric test and coaugulationPlasmatic proteins and enzymes in clinical diagnosis. Evaluation of relevant renal, cardiac and hepatic diseases.Evaluation and comprehension of hydroelectrolytic balance. Evaluation of relevant endocrine alterations
Reference texts	G. Federici, Medicina di laboratorio. McGraw-Hill Editore, 2014. Pontieri - Russo - Frati e AA.VV., Patologia generale e fisiopatologia generale - II volume. Piccin Editore. Antonozzi I, Gulletta E. Medicina di Laboratorio. Logica & Patologia clinica. Piccin Editore 2012.
Educational objectives	This course represents the first teaching where basic knowledge such as Biochemistry are correlated to General Pathology and Pathophysiology. Students will be able to understand and decode information from laboratories and associate them to pathophysiological events and define the correct diagnosis, avoiding data misinterpretation. The main competences of students will be the correct evaluation of biomedical parameters in renal, cardiac, hepatic, immunological and haematological diseases and to have the appropriate knowledge for the use of blood products
Prerequisites	Very important: Microbiology, Fisiology, Pathology, and Immunology
Teaching methods	Face-to-face and seminar lectures with audiovisual material on all subjects of the Course
Other information	No additional information
Learning verification modality	Oral examination (2 queries/15 minutes for  "Microbiologia clinica" and "Patologia Clinica") and written quiz (for "Biochimica clinica).
Extended program	Introduction to Pathology and variability in laboratory medicine. Hemochromocytometric test: anemia and number abnormalities of leucocytes and platelets. Coaugulation: physiology of coagulation, laboratory medicine of coagulation disorders. Markers in Oncology. Molecular biology and genetic tests for diagnosis of tumors. Plasmatic proteins: alteration of plasmatic proteins and their diagnostic significance. Enzymes in clinical diagnosis: evaluation of cardiac and hepatic diseases. Transfusion and relative laboratory medicine approaches. Kidney: evaluation of relevant kidney diseases. Urine analysis: normal composition and alteration pathogenesis. Evaluation and comprehension of hydroelectrolytic balance and hemogasanalysis interpretation Evaluation of sodium, potassium homeostasis and alteration. Acidosis and alkalosis. Calcium, magnesium and phosphorus metabolisms. Evaluation of relevant endocrine alterations

PROFESSIONALISING TRAINING IN CLINICAL MICROBIOLOGY

Code	GP005715
Location	PERUGIA
CFU	1
Teacher	Antonella Mencacci
Teachers	Antonella Mencacci
Hours	25 ore - Antonella Mencacci
Learning activities	Altro
Area	Tirocini formativi e di orientamento
Academic discipline	MED/07
Type of study-unit	Obbligatorio (Required)
Language of instruction	Italian
Educational objectives	Knowledge of appropriate tests to diagnose bacterial and viral infections.

PROFESSIONALISING TRAINING IN CLINICAL PATHOLOGY

Code	GP005714
Location	PERUGIA
CFU	1
Teacher	Stefano Brancorsini
Teachers	Stefano Brancorsini
Hours	25 ore - Stefano Brancorsini
Learning activities	Altro
Area	Tirocini formativi e di orientamento
Academic discipline	MED/05
Type of study-unit	Obbligatorio (Required)
Language of instruction	Italian
Contents	Setup and organization of a medicine laboratory. Input and output of laboratory. Analysis of analytical data and their evaluation
Reference texts	G. Federici, Medicina di laboratorio. McGraw-Hill Editore, 2014. Pontieri - Russo - Frati e AA.VV., Patologia generale e fisiopatologia generale - II volume. Piccin Editore. Antonozzi I, Gulletta E. Medicina di Laboratorio. Logica & Patologia clinica. Piccin Editore 2012.
Educational objectives	This course represents the first teaching where basic knowledge such as Biochemistry are correlated to General Pathology and Pathophysiology. This internship has the aim to elaborate relevant topics and to knowledge specific problems about organization and correct functioning of diagnostic laboratory to better understand the analytical data and evaluation of clinical information.
Prerequisites	Very important: Biology, Fisiology, pathology, and immunology.
Teaching methods	Seminars for large groups of studentsAccess of small groups of students in the medical laboratories.
Other information	No additional information
Learning verification modality	Oral examination (2 queries/15 minutes for each "modulo".
Extended program	The internship has the aim to elaborate practically specific topics of the programme already reported in the module of Clinical Pathology. This course will be done by seminars for large groups of students and by access of small groups of students in the medical laboratories.