Unit BIOCHEMISTRY AND CLINICAL BIOCHEMISTRY

BIOCHEMISTRY

Code	A001509
CFU	2
Teacher	Veronica Ceccarelli
Teachers	Veronica Ceccarelli
Hours	24 ore - Veronica Ceccarelli
Learning activities	Base
Area	Scienze biomediche
Academic discipline	BIO/10
Type of study-unit	Obbligatorio (Required)
Language of instruction	italian
Contents	Carbohydrates, lipids, aminoacids and proteins  Vitamins and Coenzymes  Structure and functions of membranes  Enzymes  Bioenergetics  The tricarboxylic acid cycle  Carbohydrate metabolism  Lipid metabolism  Digestion and absorption of lipids.  Metabolism of proteins and amino acids  Hormones
Reference texts	Biochimica e Biologia per le professioni sanitarie Rita Roberti - Giovanni Alunni Bistocchi-Cinzia  Antognelli- Vincenzo Nicola TalesaMcGraw-Hill
Educational objectives	Main points to be understood: Digestion and biolocical oxidations; the respiratory chain; metabolism of carbohydrates; membranes and signal transduction; hormones; biochemistry of tissues.
Prerequisites	In order to be able to understand and apply the majority of the contents described within the course, the student must have acquired basic knowledge of cellular biology and chemistry
Teaching methods	The course is organized with face to face lessons on all subjects of the course using slide projections. Students are stimulated to interact with the teacher during the lectures.
Other information	The attendance is compulsory at least 75% of lessonsRoom bulding C polo universitario -Pzza L. Severi S. Andrea delle Fratte Perugia
Learning verification modality	Oral exam
Extended program	Carbohydrates, lipids, aminoacids and proteins  Vitamins and Coenzymes  Structure and functions of membranes  Enzymes  Bioenergetics  The tricarboxylic acid cycle  Carbohydrate metabolism  Lipid metabolism  Digestion and absorption of lipids.  Metabolism of proteins and amino acids  Hormones

CLINICAL BIOCHEMISTRY

Code	A001510
CFU	2
Teacher	Antonella Barbati
Teachers	Antonella Barbati
Hours	24 ore - Antonella Barbati
Learning activities	Base
Area	Scienze biomediche
Academic discipline	BIO/12
Type of study-unit	Obbligatorio (Required)
Language of instruction	Italian
Contents	Definition and finality of the Clinical Biochemistry. The laboratory and the analytical sectors; the Point of Care Testing. The biological samples: manipulation, transport and storage of the samples. Clinical Enzimology; Analysis for organ profiles : Liver and Kidney; the urines analysis. Markers of the IMA. Hormones and endocrin system. Antibodies monoclonali and them application in the Clinical Biochemistry.
Reference texts	Slides and didattic material
Educational objectives	The programme is designed to provide students with a grounding in the theory and practice of clinical biochemistry.
Prerequisites	knowledge of basic biochemistry
Teaching methods	face-to-face lesson
Other information	e-mail: antonella.barbati@unipg.it
Learning verification modality	oral exam
Extended program	Definition and finality of the Clinical Biochemistry. The biological samples: blood (Whole blood, plasma, serum), urines, sinovial, amniotic and peritoneal fluids, etc.; The pre-analitical variability: preparation and conditions of the patient; manipulation, transport and storage of the sample. The hemolysis and its effects on the analytical determinations. The protidogram. Clinical Enzimology: enzymes and isoenzymes. Analysis for organ profiles : Liver and Kidney; the urines analysis. Markers of the IMA: concept of diagnostic window and interpretation of their serum levels. Hormones and endocrin system. Antibodies monoclonali and them application in the Clinical Biochemistry.

ELEMENTS OF INSTRUMENT CHEMISTRY

Code	A001512
CFU	1
Teacher	Giampiero Scortichini
Teachers	Giampiero Scortichini
Hours	16 ore - Giampiero Scortichini
Learning activities	Caratterizzante
Area	Scienze della prevenzione e dei servizi sanitari
Academic discipline	MED/50
Type of study-unit	Obbligatorio (Required)
Language of instruction	Italian
Contents	The course is focused on the principles of chromatographic separation, high performance liquid chromatography (HPLC) and gas chromatography instrumentation, including their general applications. Particular emphasis to HPLC and GC coupling with mass spectrometry (MS) is given, highlighting MS theoretical principles and techniques.
Reference texts	The study for the exam will be focused on the documents provided by the teacher, published on www.unistudium.unipg.it/unistudium/login/index.php Students may find it helpful to consult the following texts to learn more about the course contents: D.A. Skoog, J.F. Holler, S.R. Crouch. Principles of instrumental analysis. Brooks/Cole Pub Co, 2017. J. Graves, J. Roboz. Mass spectrometry for the novice. Taylor & Francis Group, 2014.
Educational objectives	Students acquire knowledge about the analytical principles and the instrumentation used in liquid and gas chromatography and related detection systems, with particular reference to the combination of chromatographic systems with mass spectrometers. They have basic information on the potential, limits and general applications of the aforementioned instrumentation in the analytical laboratory, including data processing for qualitative and quantitative analysis. Students gain experience with respect to the operational phases that constitute analytical methods based on the use of chromatographic systems, becoming familiar with the proposed laboratory applications.
Prerequisites	In general, the knowledge and skills acquired in the following courses are required: General Chemistry and biochemical propedeutics; Electrical and electronic measurements. In particular, students must possess elementary notions of optics and elementary algebra, be able to deal with simple mathematical functions, know the nomenclature of the most common chemical compounds and know how to perform simple stoichiometric calculations.
Teaching methods	Frontal lessons accompanied by exercises and simulations. Laboratory demonstration of selected analytical applications.
Other information	For contacts: giampiero.scortichini@unipg.it
Learning verification modality	The examination related to this module takes place in oral form together with the modules of "Biochemistry", "Clinical Biochemistry" and "Organization of the clinical biochemistry laboratory". There is normally one question for each module. For information on support services for students with disabilities and / or DSA please visit the page http://www.unipg.it/disabilita-e-dsa
Extended program	Detailed course program: Principles of chromatographic separation and various types; Gas chromatography (GC): system operation, separation columns and detectors. High performance liquid chromatography (HPLC): system operation, separation columns and detectors. Qualitative and quantitative analysis in GC and HPLC, examples of applications. Mass spectrometry (MS): theoretical principles, mass spectrometer coupling to GC and HPLC systems, ion sources and methods of ionization, mass analyzers, analytical techniques and strategies. Qualitative and quantitative analysis in GC-MS and HPLC-MS, examples of applications.

ORGANIZATION OF THE LABORATORY OF CLINICAL BIOCHIMICA

Code	A001511
CFU	2
Teachers	Silvia Covalovo (Codocenza)
Hours	20 ore (Codocenza) - Silvia Covalovo
Learning activities	Caratterizzante
Area	Scienze e tecniche di laboratorio biomedico
Academic discipline	MED/46
Type of study-unit	Obbligatorio (Required)
Language of instruction	Italiano
Contents	Illustration of the purposes of laboratory medicine. Organization of the work cycle in a laboratory type of first level diagnostics. Illustration of the concepts of quality and analytical variability as fundamental concepts of the course and of the laboratory. Methods and indicators to assess the reliability of analytical and diagnostic laboratory data.
Reference texts	Material provided by teacher www.unistudium.unipg.it/unistudium/login/index.php
Educational objectives	At the end of the course the student will have acquired knowledge about the mission and organization of the clinical laboratory. It should also be familiar with the phases of the total testing process and the key indicators needed to measure and monitor the quality of laboratory data. The learning of these elements will be key to the future professional to a critical and conscious activity of TLB to ensure at all times for their skills, the analytical quality of the entire process leading to the production of the data.
Prerequisites	The examination of "General chemistry and biochemical propedeutics", is required
Teaching methods	Frontal lessons on all matters under the Programme. Are planned exercises on clinical case on the application of the main indices useful to verify the reliability of the analytical and diagnostic laboratory data.
Learning verification modality	Biochemistry" and "Clinical Biochemistry". There is usually a question for each module.
Extended program	THE MEDICAL LABORATORY AND CLINICAL-DIAGNOSTIC LABORATORY; The medical laboratori: definition and evolution; Mission;The medical laboratory role on prevention diagnosis, treatment and rehabilitation cicle Staff; Instrumentation; Infrastructure Organization of the clinical laboratory: activity; analytical areas; Analytical-diagnostic pathways The total testing process; The T.A.T. (Turn Around Time). DEFINITIONANDTYPESOFLABORATORY TESTS ANALYTICAL RESULTS: VARIABILITY OF ANALYTICAL RESULTS AND QUALITY OF ANALYTICAL RESULTS The analytical cycle; biological variabilità; analytical variability Reliability oflaboratory results sensitivity, specificity, accuracy, precision The errors (coarse, random, systematic) Internal Quality Control (CQI); External quality assessment (VEQ) DIAGNOSTIC RELIABILITY OF LABORATORY RESULTS Clinical relevance of analytical data: main index diagnostic reliability; Method of calculation of the main indices of diagnostic reliability. VARIABILITY'BIOLOGICAL AND COMPARISON VALUES Concept of value / reference interval; Method for the construction of a reference interval Criteria and characteristics of the report EVALUATION OF DIAGNOSTIC TEST