Veterinary medicine
Study-unit Code
In all curricula
Mauro Coletti
  • Mauro Coletti
  • 65 ore - Mauro Coletti
Course Regulation
Coorte 2017
Learning activities
Discipline delle malattie infettive ed infestive
Academic discipline
Type of study-unit
Obbligatorio (Required)
Type of learning activities
Attività formativa monodisciplinare
Language of instruction
italian language
Morphology,structure and function of bacterial cells.Cultivation and replication of bacteria.Bacterial colonization, tissue invasion.Classification of bacteria.Virus features.Replication of viruses.Classification of viruses.Innate and acquired immunity.Humoral immunity.Cell-mediated immunity.Passive immunity. Vaccines.
Reference texts
TextbookPoli G., Dall'Ara P., Martino P.A., Rosati S. Microbiologia e immunologia veterinaria. Edra S.p.A, 2017
Recommended booksTizard I.R. Veterinary Immunology. An Introduction. Ninth Edition, Saunders Elsevier 2013Day M.J., Veterinary Immunology. Principles and Practice. Second Edition. CRC Press. 2014During the theoretical activity, slides and CDs are used. Other teaching materials can be found on the University website.
Educational objectives
Know. To acquire the concepts of General Microbiology with particular reference to bacteria and viruses of veterinary interest and the manner of microorganisms by means of which are capable of inducing infection and disease in animals. In addition, students must make their own the basics of immunology that he may know and understand the importance of serological tests used to study microorganisms and for the diagnosis of infectious diseases. The student must also acquire the concepts of direct and indirect prophylaxis.Knowing how to do: the student must be able to interpret immune responses to microorganisms, to evaluate the use of different types of vaccines. It must also prepare and present a presentation on a bacteria species or a viral family.
In order to understand subject matter teaching is indispensable passing the exams of Biochemistry.
Teaching methods
Theoretical activity: in the classroom lectures on all subjects of the program;

Practical activity: in the laboratory of Microbiology, in this case, students will be divided into groups. The topics will cover cultivation and isolation of microorganisms, their identification and use of serological tests in the diagnosis of infectious diseases.Self-direct learning: Preparing a presentation on a bacteria species or a viral family based on on-line research and led by the teacher.
Other information
Frequenza: Compulsory (70%)Sede: Department, via S. Costanzo 4, Perugia. The educational activities will be carried out as reported on the website http://www.medvet.unipg.it/Date di inizio e termine delle attività didattiche: Beginning and end as reported on the website of the Department of Veterinary Medicine: http://www.medvet.unipg.itCalendario delle attività didattiche: Lectures and Practical lessons according to the timetable available at http://www.medvet.unipg.it/Attività di didattica integrativa: It is scheduled tutoring activities by arrangement with the teacher
Learning verification modality
The exam includes an oral examination lasting about 45 minutes, during which will be verified knowledge and the processing capability of the same, as well as the properties of language by students, relatively subject matter teaching imparted. The test must also highlight the student's ability to apply their knowledge.
Extended program
THEORETICAL LESSONSIntroduction to the study of microbiology (1 hour).Structure and function of the bacterial cell surface structures, appendages bacterial intracytoplasmic structures, bacterial spore (2 hours). Reproduction of bacteria, environmental and nutritional factors that affect bacterial growth (2 hours). Genetics of bacteria: mutations, genetic recombination (transformation, transduction, phage conversion, conjugation) (2 hours). Bacteria-interactions ospite.Proprietà pathogenic germs: factors of colonization, diffusion factors, factors that interfere with phagocytosis, toxic factors (3 hours).Physical and chemical agents in the control of microorganisms. Antimicrobial agents (1 hour). Laboratory diagnosis of bacterial diseases (2 hours). Classification of bacteria: classification criteria (1 hour). Bacteriology special kinds: Staphylococcus, Streptococcus, Bacillus, Mycobacterium, Escherichia, Shigella, Salmonella, Klebsiella, Proteus, Yersinia, Pasteurella, Mannheimia, Brucella, Pseudomonas, Burkholderia, Clostridium, Leptospira, Mycoplasma, Chlamydia, Rickettsia (3 hours).Morphology and structure of virus (2 hours).Bacteriophages (1 hour). Virus-host relationships: how to transmit viruses, routes of entry and spread, types of viral infection 2 hours). Stages of the viral infection (2 hours). Defense mechanisms of cells: viral interference (1 hour). Laboratory diagnosis of viral diseases (1 hour). Classification of viruses: classification criteria (1 hour). Virology special families: Parvoviridae, Circoviridae, Adenoviridae, Herpesviridae, Asfarviridae, Poxviridae, Picornaviridae, Caliciviridae, Reoviridae, Togaviridae, Flaviviridae, Orthomyxoviridae, Paramyxoviridae, Rhabdoviridae, Coronaviridae, Arteriviridae, Retroviridae, Arenaviridae, Bunyaviridae (3 hours).Antigens and immunogenicity: antigens of microorganisms and animal cells (2 hours).Non-specific innate defense systems: physical and chemical barriers, cellular mechanisms, humoral factors (2 hours). Defensive systems acquired and specific. Immune cells: B cells, T cells, dendritic cells (2 hours).Processing and presentation of antigen. Major histocompatibility complex (1 hour). Antibodies: structure and biological characteristics (1 hour). Effector mechanisms of humoral immunity: thymus-dependent antigens and thymus-independent antigens (1 hour). Mucosal immunity or local. Passive immunity (1 hour). Serological diagnosis (1 hour). Effector mechanisms of cell-mediated immunity (1 hour). Vaccination. Types of vaccines: inactivated, live attenuated, purified composed of subunits, synthetic products with recombinant DNA technology. Adjuvants. Routes of administration of the vaccines (3 hours).PRACTICAL LESSONS1) Preparation of bacterial culture medium (2 hours)2) Seeding of sample plates and incubation (2 hours)3) Smears from bacterial colonies, Gram staining, observation prepared by optical microscope (3 hours)4) Biochemical tests (2 hours)5) Execution and evaluation of antibiogram result (2 hours)6) Cell cultures (2 hours)7) Observation by light microscopy and evaluation cytopathic effect (2 hours)8) Virus neutralization test on cell culture (3 hours)SELF DIRECTED LEARNING1) Presentation on a bacteria species or a viral family (2 hours)
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