Veterinary medicine
Study-unit Code
In all curricula
Fabrizio Passamonti
  • Fabrizio Passamonti
  • Mauro Coletti (Codocenza)
  • 30 ore - Fabrizio Passamonti
  • 35 ore (Codocenza) - Mauro Coletti
Course Regulation
Coorte 2019
Learning activities
Discipline delle malattie infettive ed infestive
Academic discipline
Type of study-unit
Obbligatorio (Required)
Type of learning activities
Attività formativa monodisciplinare
Language of instruction
Morphology,structure and function of bacterial cells.Cultivation and replication of bacteria.Bacterial colonization, tissue invasion.Classification of bacteria.Virus features.Replication of viruses.Classification of viruses.Innate and acquired immunity.Humoral immunity.Cell-mediated immunity.Passive immunity. Vaccines.
Reference texts
TextbookPoli G., Dall'Ara P., Martino P.A., Rosati S. Microbiologia e immunologia veterinaria. Edra S.p.A, 2017
Recommended booksTizard I.R. Veterinary Immunology. An Introduction. Ninth Edition, Saunders Elsevier 2013Day M.J., Veterinary Immunology. Principles and Practice. Second Edition. CRC Press. 2014During the theoretical activity, slides and CDs are used. Other teaching materials can be found on the University website.
Educational objectives
Know. To acquire the concepts of General Microbiology with particular reference to bacteria and viruses of veterinary interest and the manner of microorganisms by means of which are capable of inducing infection and disease in animals. In addition, students must make their own the basics of immunology that he may know and understand the importance of serological tests used to study microorganisms and for the diagnosis of infectious diseases. The student must also acquire the concepts of direct and indirect prophylaxis.Knowing how to do: the student must be able to perform and send diagnostic samples in the laboratory. He must also know how to interpret the results of microbiological investigations, as well as the immune responses to microorganisms and to evaluate the use of different types of vaccines.It must also prepare and present a presentation on a bacteria species or a viral family.
In order to understand subject matter teaching is indispensable passing the exams of Biochemistry.
Teaching methods
Theoretical activity: in the classroom lectures on all subjects of the program;

Practical activity: in the laboratory of Microbiology, in this case, students will be divided into groups. The topics will cover cultivation and isolation of microorganisms, their identification and use of serological tests in the diagnosis of infectious diseases.Self-direct learning: Preparing a presentation on a bacteria species or a viral family based on on-line research and led by the teacher.
Other information
Frequenza: Compulsory (70%)Sede: Department, via S. Costanzo 4, Perugia. The educational activities will be carried out as reported on the website http://www.medvet.unipg.it/Date di inizio e termine delle attività didattiche: Beginning and end as reported on the website of the Department of Veterinary Medicine: http://www.medvet.unipg.itCalendario delle attività didattiche: Lectures and Practical lessons according to the timetable available at http://www.medvet.unipg.it/Attività di didattica integrativa: It is scheduled tutoring activities by arrangement with the teacher
Learning verification modality
The MICROBIOLOGY AND VETERINARY IMMUNOLOGY exam consists of the following tests:

1) WRITTEN TEST: Trial test carried out at each appeal before the oral test.
Duration: 60 minutes
Type of test: written with a closed answer.
Purpose: extensive evaluation of learning.
Description - The written test consists of 40 separate questions in questions with answers to a choice between 4 possible, of which only one is the correct one. The questions cover the entire program (Bacteriology, Virology, Immunity, Bacteriology and Special Virology).
The correct answers are assigned half a point. No wrong points or no penalties are assigned to wrong answers or not given.
Test measurement: the test allows a maximum of 20 points to be obtained and is considered passed if the score obtained is equal to or greater than 12.

2) ORAL TEST: final test.
Duration: 40-60 minutes.
Description: open stimulus interview with open answer.
4 questions related to the entire program will be proposed (Bacteriology, Virology, Immunity, Bacteriology and Special Virology)

Measurement: the test is evaluated based on the following parameters:
Argumentative rigor score from <12 to 20
Completeness from <12 to 20
Language properties from <12 to 20
Depth of analysis and connection capacity - From <12 to 20
Each score corresponds to the following class of judgment:
<12 = insufficient (Widespread and serious deficiencies, inconsistencies and errors in the argument; poor and inadequate language; difficult and non-autonomous construction of the speech; failure or incorrect application of argumentative constraints and logical connections)
from 12 to 14 = sufficient (Some shortcomings, inconsistencies and errors in the argument; logical articulation of the discourse, but excessively simple and essential exposure; autonomous construction of the discourse with a very summary summary of the contents; adequate but stunted application of constraints and logical connections )
from 15 to 17 = good (Rare shortcomings, inconsistencies and errors in the argument; logical articulation of the argumentative path, autonomous construction of discourse with appropriate use of specific language; complete application of constraints and logical connections)
from 18 to 20 = excellent (Absence of deficiencies, inconsistencies and errors in the argument; logical, clear and rich articulation of the argumentative path; autonomous construction of the speech with appropriate and complete use of the specific language; complete application of the constraints and logical connections also with original choices)

The test is passed if the score obtained is equal to or greater than 48 points.

The achievement of the maximum score in all the tests will entail the attribution of the honors if the oral test proves “absolute argumentative, logical and linguistic mastery; autonomous, secure, rich and articulated construction of the discourse, supported by a significant original contribution deriving from the use of multiple study sources "

The examination in its entirety is passed if the overall score is equal to or greater than 60/100 (equal to 18/30).
For information on support services for students with disabilities and / or DSA visit the page http://www.unipg.it/disabilita-e-dsa
Extended program
Programma esteso PROGR_EST Sì LEZIONI TEORICHEIntroduzione allo studio della microbiologia ( 1 ora). Struttura e funzione della cellula batterica, strutture di superficie,appendici batteriche, strutture intracitoplasmatiche, spora batterica (2 ore).Riproduzione dei batteri, fattori nutritizi ed ambientali che condizionano la crescita batterica (2 ore). Genetica dei batteri: mutazioni, ricombinazioni genetiche (trasformazione, trasduzione, conversione fagica, coniugazione) (2 ore). Interazioni batteri-ospite (proprietà patogene dei germi): fattori di colonizzazione, fattori di diffusione, fattori che interferiscono con la fagocitosi, fattori tossici (3 ore). Agenti fisici e chimici nel controllo dei microrganismi. Agenti antimicrobici (1 ora). Diagnosi di laboratorio delle malattie batteriche 2 ore). Classificazione dei batteri: criteri di classificazione (1 ora). Batteriologia speciale generi: Staphylococcus, Streptococcus,Bacillus,Mycobacterium, Escherichia, Shigella, Salmonella, Klebsiella, Proteus, Yersinia, Pasteurella, Mannheimia, Brucella, Pseudomonas, Burkholderia, Clostridium, Leptospira, Mycoplasma, Chlamydia, Rickettsia (3 ore).
Morfologia e struttura dei virus (2 ore). Batteriofagi (1 ora).Rapporti virus-ospite: modalità di trasmissione dei virus, vie di ingresso e diffusione, tipi di infezione virale (2 ore).Fasi dell'infezione virale (2 ore). Meccanismi di difesa delle cellule: interferenza virale (1 ora). Diagnosi di laboratorio delle malattie virali (1 ora). Classificazione dei virus: criteri di classificazione (1 ora). Virologia speciale famiglie:Parvoviridae, Circoviridae, Adenoviridae, Herpesviridae, Asfarviridae, Poxviridae, Picornaviridae, Caliciviridae, Reoviridae, Togaviridae, Flaviviridae, Orthomyxoviridae, Paramyxoviridae, Rhabdoviridae, Coronaviridae, Arteriviridae, Retroviridae, Arenaviridae, Bunyaviridae (3 ore).
Antigeni ed immunogenicità: gli antigeni dei microrganismi e delle cellule animali ( 2 ore). Sistemi difensivi innati non specifici: barriere fisico-chimiche, meccanismi cellulari, fattori umorali (2 ore). Sistemi difensivi acquisiti e specifici. Cellule del sistema immunitario: linfociti B, linfociti T, cellule dendritiche (2 ore). Processazione e presentazione dell'antigene. Complesso maggiore di istocompabilità (1 ora). Anticorpi: struttura e caratteristiche biologiche ( 1 ora). Meccanismi effettori dell'immunità umorale: antigeni timo-dipendenti ed antigeni timo-indipendenti (1 ora). Immunità mucosale o locale. Immunità passiva (1 ora).Diagnostica sierologica (1 ora). Meccanismi effettori dell'immunità cellulo-mediata (1 ora). Vaccinazione. Tipi di vaccini: inattivati, vivi, purificati costituiti da subunità, sintetici, prodotti con la tecnica del DNA ricombinante. Adiuvanti. Vie di somministrazione dei vaccini ( 3 ore).LEZIONI PRATICHE1) Preparazione terreni coltura batteri (2 ore)2) Semina su piastra di campioni e loro incubazione (2 ore)3) Strisci a partire da colonie batteriche, colorazione di Gram, osservazione preparati al microscopio ottico (3 ore)4) Prove biochimiche (2 ore)5) Esecuzione e valutazione risultato antibiogramma (2 ore)6) Colture cellulari (2 ore)7) Osservazione al microscopio ottico e valutazione effetto citopatico (2 ore)8) Prova di virus neutralizzazione su coltura cellulare (3 ore)SELF DIRECTED LEARNING1) Presentazione su un genere di batteri o una famiglia virale (2 ore) THEORETICAL LESSONSIntroduction to the study of microbiology (1 hour).Structure and function of the bacterial cell surface structures, appendages bacterial intracytoplasmic structures, bacterial spore (2 hours). Reproduction of bacteria, environmental and nutritional factors that affect bacterial growth (2 hours). Genetics of bacteria: mutations, genetic recombination (transformation, transduction, phage conversion, conjugation) (2 hours). Bacteria-interactions ospite.Proprietà pathogenic germs: factors of colonization, diffusion factors, factors that interfere with phagocytosis, toxic factors (3 hours).Physical and chemical agents in the control of microorganisms. Antimicrobial agents (1 hour). Laboratory diagnosis of bacterial diseases (2 hours). Classification of bacteria: classification criteria (1 hour). Bacteriology special kinds: Staphylococcus, Streptococcus, Bacillus, Mycobacterium, Escherichia, Shigella, Salmonella, Klebsiella, Proteus, Yersinia, Pasteurella, Mannheimia, Brucella, Pseudomonas, Burkholderia, Clostridium, Leptospira, Mycoplasma, Chlamydia, Rickettsia (3 hours).Morphology and structure of virus (2 hours).Bacteriophages (1 hour). Virus-host relationships: how to transmit viruses, routes of entry and spread, types of viral infection 2 hours). Stages of the viral infection (2 hours). Defense mechanisms of cells: viral interference (1 hour). Laboratory diagnosis of viral diseases (1 hour). Classification of viruses: classification criteria (1 hour). Virology special families: Parvoviridae, Circoviridae, Adenoviridae, Herpesviridae, Asfarviridae, Poxviridae, Picornaviridae, Caliciviridae, Reoviridae, Togaviridae, Flaviviridae, Orthomyxoviridae, Paramyxoviridae, Rhabdoviridae, Coronaviridae, Arteriviridae, Retroviridae, Arenaviridae, Bunyaviridae (3 hours).Antigens and immunogenicity: antigens of microorganisms and animal cells (2 hours).Non-specific innate defense systems: physical and chemical barriers, cellular mechanisms, humoral factors (2 hours). Defensive systems acquired and specific. Immune cells: B cells, T cells, dendritic cells (2 hours).Processing and presentation of antigen. Major histocompatibility complex (1 hour). Antibodies: structure and biological characteristics (1 hour). Effector mechanisms of humoral immunity: thymus-dependent antigens and thymus-independent antigens (1 hour). Mucosal immunity or local. Passive immunity (1 hour). Serological diagnosis (1 hour). Effector mechanisms of cell-mediated immunity (1 hour). Vaccination. Types of vaccines: inactivated, live attenuated, purified composed of subunits, synthetic products with recombinant DNA technology. Adjuvants. Routes of administration of the vaccines (3 hours).PRACTICAL LESSONS1) Preparation of bacterial culture medium (2 hours)2) Seeding of sample plates and incubation (2 hours)3) Smears from bacterial colonies, Gram staining, observation prepared by optical microscope (3 hours)4) Biochemical tests (2 hours)5) Execution and evaluation of antibiogram result (2 hours)6) Cell cultures (2 hours)7) Observation by light microscopy and evaluation cytopathic effect (2 hours)8) Virus neutralization test on cell culture (3 hours)SELF DIRECTED LEARNING1) Presentation on a bacteria species or a viral family (2 hours)
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