Unit APPLIED MOLECULAR PHARMACOLOGY
- Course
- Biotechnology
- Study-unit Code
- A000990
- Curriculum
- In all curricula
- Teacher
- Marco Gargaro
- Teachers
-
- Marco Gargaro
- Hours
- 57 ore - Marco Gargaro
- CFU
- 6
- Course Regulation
- Coorte 2021
- Offered
- 2023/24
- Learning activities
- Affine/integrativa
- Area
- Attività formative affini o integrative
- Academic discipline
- BIO/14
- Type of study-unit
- Opzionale (Optional)
- Type of learning activities
- Attività formativa monodisciplinare
- Language of instruction
- Italian
- Contents
- The course consists of lectures aimed at understanding the molecular basis of the function and production of endogenous molecules, and practical classes concerning the techniques that are employed for the production of new biotechnological drugs such as recombinant proteins and enzymes.
- Reference texts
- Farmacologia Generale e Molecolare" (Clementi-Fumagalli)
Rang, Dale, Ritter, Moore “Farmacologia” C.E.A, Milano. - Educational objectives
- The main objective of course module is to provide students with the foundation for understanding the importance of the molecular and genetic studies in the design of biopharmaceuticals.
The main acquired knowledge will be:
• The molecular mechanisms of immune responses;
• The molecular basis of the use of endogenous engineered proteins as drugs.
The main gained skills will be:
• Construction of specific primers also using specific bioinformatic tools;
• Techniques of validation of the molecular functions of a specific drug. - Prerequisites
- In order to be able to understand and take the exam of the course, it is mandatory to have successfully taken previously the Physiology and Biochemistry exams.
- Teaching methods
- Theoretical lessons and practical training.
- Other information
- Following classes is optional but strongly advised.
- Learning verification modality
- The examination includes an oral test, which consists of a discussion lasting approximately twenty minutes. This discussion aims to assess how well the student knows and understands the theoretical content present in the Applied Molecular Pharmacology program. Furthermore, during the examination, it will be determined whether the student has acquired the essential practical methodologies required to identify molecular targets during the drug development process.
Students with disabilities and/or DSA are invited to visit the page dedicated to the tools and measures envisaged and to agree in advance what is necessary with the teacher (https://www.unipg.it/disabilita-e -dsa). - Extended program
- Theoretical lessons:
Molecular aspects of immune responses and immune tolerance. Cytokine receptors as targets for immunomodulatory agents. Drugs for rare genetic diseases. Pharmacotherapy of diabetes. Intracellular receptors.
Pharmacological use of synthetic oligonucleotides. Gene therapy. The vectors and methods of gene transfer.
Practical lessons:
Manipulation and culture of macrophage cells. RNA extraction and cDNA synthesis. PCR and electrophoresis. Protein quantification and Western blotting. Direct and indirect ELISA tests. Nitric oxide determination test. - Obiettivi Agenda 2030 per lo sviluppo sostenibile
- Health and wellness