Unit BIOCHEMISTRY AND CLINICAL BIOCHEMISTRY

BIOCHEMISTRY

Code	A001509
CFU	2
Teacher	Mirco Dindo
Teachers	Mirco Dindo
Hours	24 ore - Mirco Dindo
Learning activities	Base
Area	Scienze biomediche
Academic discipline	BIO/10
Type of study-unit	Obbligatorio (Required)
Language of instruction	Italian
Contents	Molecules of biological interest. Metabolic pathways. Krebs cycle. Respiratory chain. Oxidative phosphorylation. Metabolism of carbohydrates, lipids, and aminoacids.
Reference texts	Biochimica e Biologia per le professioni sanitarie - Roberti, Alunni-Bistocchi, Antognelli, Talesa - McGraw-Hill Le basi della biochimica-Champe-Ferrier-Zanichelli Chimica e biochimica-Samaja-Paroni-Piccin Chimica e Biochimica- Bertoldi- Colombo-Magni-Marin-Palestini-EdiSES Nelson-Cox Introduzione alla Biochimica di Lehninger-Zanichelli
Educational objectives	The main aim of this course is to provide students with the knowledge of basic properties of biological systems and of the mechanisms controlling cellular homeostasis. Main knowledge acquired will be: - energy transfer in biochemical transformations; - structure and function of molecules involved in cell metabolism; -main metabolic transformations; - hormonal regulation of metabolism in feeding and fasting. The main competence will be: - to evaluate the nature and properties of reagents and solutions usually employed in professional practice; - to understand the general metabolic behavior in physiopathological conditions
Prerequisites	The main aim of this course is to provide students with the knowledge of basic properties of biological systems and of the mechanisms controlling cellular homeostasis. Main knowledge acquired will be: - energy transfer in biochemical transformations; - structure and function of molecules involved in cell metabolism; -main metabolic transformations; - hormonal regulation of metabolism in feeding and fasting. The main competence will be: - to evaluate the nature and properties of reagents and solutions usually employed in professional practice; - to understand the general metabolic behavior in physiopathological conditions
Teaching methods	The course is organized in lectures on all the subjects. Students are stimulated to interact with the teacher during the lectures.
Other information	Seat: Polo didattico S. Andrea delle fratte-Centro didattico Consulting: Studio, edificio D 2° piano, via Gambuli, S. Andrea delle Fratte
Learning verification modality	The exam consists of a written test possibly followed by an oral examination relative to the three modules of the integrated course. The written test, comprising basic questions covering all the topics, aims to assess the minimum basic preparation to face the oral examination. WRITTEN TEST: 10 questions with multiple answers + 2 open questions. The oral exam aims to assess the level of knowledge of the subjects of the integrated course, and the ability to make connections and to integrate knowledge, also in relation to the nursing professional activity. The oral exam will also test the student communication skills.
Extended program	ELEMENTS OF GENERAL BIOCHEMISTRY. Carbohydrates. Structure and properties of monosaccharides, disaccharides, and polysaccharides. The glycosidic bond. Carbohydrates of physiologic significance: glucose, fructose, ribose, saccharose, lactose, starch, cellulose, glycogen. Lipids. Structure and properties of fatty acids, acylglycerols, phospholipids, glycolipids, and cholesterol. Aminoacids. Peptidic bond. Proteins: structural organization, classification, functions. Denaturation of proteins. Fibrous proteins: keratin and collagen. Structure and functions of hemoglobin and myoglobin, oxygen transport, allosteric modulators. Physiologic and pathologic hemoglobins. Reaction rate. Enzymes as biological catalysts. Effect of substrate concentration, pH, and temperature. Hints on enzyme activity regulation. Clinical importance of enzymes in serum. Hints on vitamins and coenzymes (NAD, FAD). ELEMENTS OF DYNAMIC BIOCHEMISTRY. Overview of metabolic pathways: anabolism, catabolism, linear and cyclic pathways. ATP: structure and role in the metabolism

CLINICAL BIOCHEMISTRY

Code	A001510
CFU	2
Teacher	Valerio Malagigi
Teachers	Valerio Malagigi
Hours	24 ore - Valerio Malagigi
Learning activities	Base
Area	Scienze biomediche
Academic discipline	BIO/12
Type of study-unit	Obbligatorio (Required)
Language of instruction	Italiano
Contents	Mission del laboratorio analisi. Test di riferimento nelle patologie riguardanti i sistemi: cardiocircolatorio, emopoietico, epatico, pancreatico, tiroideo e coagulativo
Reference texts	Slide inerenti le lezioni frontali. sono previsti percorsi, materiali e/o metodologie di accertamento coerenti con le necessità degli studenti disabili e/o DSA
Educational objectives	Conoscenza della Mission di laboratorio, chi e perchè richiede esami di laboratorio. Generalità su tipo di campione e tipo provetta, TAT, referto di laboratorio. Conoscenza delle principali analisi di biochimica clinica eseguibili nei vari campioni biologici e del loro significato in relazione alla funzionalità/ integrità degli organi/tessuti di riferimento.
Prerequisites	E' auspicabile che lo studente abbia già conoscenze riguardo la fisologia umana, le tecniche di laboratorio inoltre l'insegnamento di "Chimica generale e laboratorio di chimica” (I anno, I semestre) è propedeutico.
Teaching methods
Other information	Il ricevimento degli studenti è possibile a fine di ogni lezione.
Learning verification modality	E' previso un esame scritto: - 5 domande a risposta chiusa per ogni modulo con quattro opzioni di risposta di cui solo una esatta del valore di 1 punto ciascuna; - 1 domanda a risposta aperta per ogni modulo del valore di 10 punti. sono previsti percorsi, materiali e/o metodologie di accertamento coerenti con le necessità degli studenti disabili e/o DSA
Extended program	Mission del laboratorio analisi. Esami diagnostici, di screening, di follow up, di stratitificazione del rischio. Chi e perchè richiede test di laboratorio, Medici di reparto, Medici di Medicina generale, Specialisti, medico del lavoro. Tempistiche di riposta (urgenze, routine) Test di riferimento nelle patologie riguardanti i sistemi: cardiocircolatorio, emopoietico, epatico, pancreatico, tiroideo e coagulativo.

ORGANIZATION OF THE LABORATORY OF CLINICAL BIOCHIMICA

Code	A001511
CFU	2
Teacher	Silvia Covalovo
Teachers	Silvia Covalovo
Hours	20 ore - Silvia Covalovo
Learning activities	Caratterizzante
Area	Scienze e tecniche di laboratorio biomedico
Academic discipline	MED/46
Type of study-unit	Obbligatorio (Required)
Language of instruction	Italiano
Contents	Illustration of the purposes of laboratory medicine. Organization of the work cycle in a laboratory type of first level diagnostics. Illustration of the concepts of quality and analytical variability as fundamental concepts of the course and of the laboratory. Methods and indicators to assess the reliability of analytical and diagnostic laboratory data.
Reference texts	Material provided by teacher www.unistudium.unipg.it/unistudium/login/index.php
Educational objectives	At the end of the course the student will have acquired knowledge about the mission and organization of the clinical laboratory. It should also be familiar with the phases of the total testing process and the key indicators needed to measure and monitor the quality of laboratory data. The learning of these elements will be key to the future professional to a critical and conscious activity of TLB to ensure at all times for their skills, the analytical quality of the entire process leading to the production of the data.
Prerequisites	The examination of "General chemistry and biochemical propedeutics", is required To take the partial exam of the "Clinical Biochemistry" and "Organisation of the clinical biochemistry laboratory" modules, it is necessary to have passed the partial exam of the "Biochemistry" module
Teaching methods	Frontal lessons on all matters under the Programme. Are planned exercises on clinical case on the application of the main indices useful to verify the reliability of the analytical and diagnostic laboratory data.
Learning verification modality	Biochemistry" and "Clinical Biochemistry". There is usually a question for each module.
Extended program	THE MEDICAL LABORATORY AND CLINICAL-DIAGNOSTIC LABORATORY; The medical laboratori: definition and evolution; Mission;The medical laboratory role on prevention diagnosis, treatment and rehabilitation cicle Staff; Instrumentation; Infrastructure Organization of the clinical laboratory: activity; analytical areas; Analytical-diagnostic pathways The total testing process; The T.A.T. (Turn Around Time). DEFINITIONANDTYPESOFLABORATORY TESTS ANALYTICAL RESULTS: VARIABILITY OF ANALYTICAL RESULTS AND QUALITY OF ANALYTICAL RESULTS The analytical cycle; biological variabilità; analytical variability Reliability oflaboratory results sensitivity, specificity, accuracy, precision The errors (coarse, random, systematic) Internal Quality Control (CQI); External quality assessment (VEQ) DIAGNOSTIC RELIABILITY OF LABORATORY RESULTS Clinical relevance of analytical data: main index diagnostic reliability; Method of calculation of the main indices of diagnostic reliability. VARIABILITY'BIOLOGICAL AND COMPARISON VALUES Concept of value / reference interval; Method for the construction of a reference interval Criteria and characteristics of the report EVALUATION OF DIAGNOSTIC TEST