Unit BIOCHEMICAL METHODS
- Course
- Biology
- Study-unit Code
- GP004082
- Curriculum
- Biomolecolare
- Teacher
- Elisabetta Chiaradia
- Teachers
-
- Elisabetta Chiaradia
- Serena Porcellati (Codocenza)
- Hours
- 10 ore - Elisabetta Chiaradia
- 37 ore (Codocenza) - Serena Porcellati
- CFU
- 6
- Course Regulation
- Coorte 2024
- Offered
- 2024/25
- Learning activities
- Affine/integrativa
- Area
- Attività formative affini o integrative
- Academic discipline
- BIO/10
- Type of study-unit
- Opzionale (Optional)
- Type of learning activities
- Attività formativa monodisciplinare
- Language of instruction
- Italian
- Contents
- Chromatographic techniques. Enzyme assays.
- Reference texts
- Wilson K., Walker J., Biochimica e biologia molecolare, Principi e tecniche EDs. 2019, Casa Editrice Raffaello Cortina. Maccarrone M., Metodologie Biochimiche e Biomolecolari, Casa Editrice Zanichelli. Bonaccorsi di Patti M.C., Contestabile R., Di Salvo M.L., Metodologie biochimiche, Casa Editrice Zanichelli.
- Educational objectives
- Knowledge about chromatographic methods for main biomolecules study.
- Prerequisites
- Basic knowledge in the field of Organic Chemistry and Biochemistry.
- Teaching methods
- Lectures.
- Other information
- Optional.
- Learning verification modality
- Interview on course topics.
- Extended program
- Lab's instruments. How to prepare solutions. Protein extraction and cellular lysis by tissue's omogeneization. Methods and techniques for protein purification: centrifugazion, fractional protein's precipitation, dialysis. Chromatographic techniques: introduction. Molecular exclusion chromatography. Ion exchange chromatography. Affinity chromatography. Partition chromatography: thin-layer chromatography. Adsorption chromatography. High-pressure liquid chromatography. Enzyme kinetics: measure of enzyme catalitic activity. Specific activity, Km and Vmax values. Enzymatic inhibition.