Unit DIAGNOSIS OF GENETIC AND HEMATOLOGICAL DISEAS

MOLECULAR BIOLOGY AND RECOMBINANT TECHNOLOGIES

Code	50993101
CFU	1
Teacher	Paolo Gorello
Teachers	Paolo Gorello
Hours	12 ore - Paolo Gorello
Learning activities	Base
Area	Scienze biomediche
Academic discipline	BIO/12
Type of study-unit	Obbligatorio (Required)
Language of instruction	Italian
Contents	Selection of biological samples ( DNA, RNA and Proteins) and quality control analysis PCR: Theoretical bases and set-up of PCR reactions Database: nucleotide and amino acid sequences Molecular Cloning: Nested PCR, RACE-PCR, TA-Cloning, expression vectors and applications Real-Time PCR: Theoretical bases, Fluorogenic probes, endogenous housekeeping genes DNA sequencing and mutational analysis : Sanger method , Next Generation Sequencing and applications.
Reference texts	Slides and notes provided by teacher
Educational objectives	The main aim of this teaching is to provide students with the theoretical principles that are the basis of the most common techniques of molecular biology, in order to identify possible applications in diagnosis.
Prerequisites	The course requires basic knowledge of Biochemistry, Cell Biology and Genetics.
Teaching methods	Lectures on all subjects of the couse.
Learning verification modality	Written Test
Extended program	Selection of biological samples ( DNA, RNA and Proteins) and quality control analysis PCR: Theoretical bases and set-up of PCR reactions Database: nucleotide and amino acid sequences Molecular Cloning: Nested PCR, RACE-PCR, TA-Cloning, expression vectors and applications Real-Time PCR: Theoretical bases, Fluorogenic probes, endogenous housekeeping genes DNA sequencing and mutational analysis : Sanger method , Next Generation Sequencing and applications.
Obiettivi Agenda 2030 per lo sviluppo sostenibile	Good Health and well-being

DIAGNOSIS OF HEMATOLOGICAL DISEAS

Code	A003232
CFU	3
Teacher	Paolo Sportoletti
Teachers	Filomena De Falco (Codocenza) Paolo Sportoletti
Hours	8 ore (Codocenza) - Filomena De Falco 20 ore - Paolo Sportoletti
Learning activities	Caratterizzante
Area	Scienze interdisciplinari cliniche
Academic discipline	MED/15
Type of study-unit	Obbligatorio (Required)
Language of instruction	Italian
Contents	The module in question is intended to bring to the attention of the student the most important hematologic disorders, new diagnostic tests and their correlation with specific diseases. Provides the necessary elements in order to interpret the meaning of changes in the main laboratory tests
Reference texts	MALATTIE DEL SANGUE E DEGLI ORGANI EMOLINFOPOIETICI- Sante Tura - Editrice ESCULAPIO - latest edition materials and/or assessment methodologies consistent with the needs of disabled and/or DSA students are available
Educational objectives	It aims to raise awareness and understanding of the student the meaning of laboratory tests for the purpose of major diseases. In particular, it aims to make early detection of diseases that may emerge from the evaluation of the laboratory tests at the end of the course the student should be able to use the acquired knowledge to identify the most common hematologic diseases
Prerequisites	N/A
Teaching methods	Lectures with presentation of reports and case studies that will give students the chance to meet with real cases in order to learn to be inferred from laboratory data sottese diseases and problems related to them. materials and/or assessment methodologies consistent with the needs of disabled and/or DSA students are available
Learning verification modality	The learning and the degree of preparation is checked by oral examination concerning the arguments discussed during the lessons. The students must demonstrate that they have acquired adequate knowledge and skills to recognize the main hematological diseases and related alterations of laboratory tests. The mark obtained for the Blood Diseases module will contribute to the final mark of the Clinical Sciences Course obtained by arithmetic mean between the marks reported in the individual modules of the Course. materials and/or assessment methodologies consistent with the needs of disabled and/or DSA students are available
Extended program	1) Hematopoiesis: concepts of general biology and laboratory techniques related to the identification and study of hematopoietic stem cells normal and pathological conditions. 2) leukemogenesis: general overview and pathogenetic mechanisms: laboratory tests related to diagnosis, prognosis, response to treatment and monitoring over time. 3) acute myeloid leukemia and acute leukemias LYMPHOID: generali. Esami concepts inherent laboratory diagnosis, prognosis, response to treatment and monitoring over time. 4) lymphoproliferative syndromes CHRONIC: general overview and differential diagnosis between the various lymphoproliferative diseases 5) chronic myeloproliferative disorders: general overview and differential diagnosis between the various myeloproliferative diseases
Obiettivi Agenda 2030 per lo sviluppo sostenibile	Health and Wellness

GENETICS

Code	50668601
CFU	1
Teacher	Paolo Prontera
Teachers	Paolo Prontera
Hours	12 ore - Paolo Prontera
Learning activities	Base
Area	Scienze biomediche
Academic discipline	MED/03
Type of study-unit	Obbligatorio (Required)

GENETICS TECNIQUES

Code	A003231
CFU	1
Teacher	Cristina Gradassi
Teachers	Cristina Gradassi
Hours	12 ore - Cristina Gradassi
Learning activities	Caratterizzante
Area	Scienze e tecniche di laboratorio biomedico
Academic discipline	MED/46
Type of study-unit	Obbligatorio (Required)