Unit CARACTERIZING INTERDISCIPLINARY LABORATORY 1 - CELLULAR - MOLECULAR
- Course
- Biological sciences
- Study-unit Code
- A003467
- Curriculum
- Cellulare-molecolare
- Teacher
- Lorena Urbanelli
- Teachers
-
- Lorena Urbanelli
- Hours
- 24 ore - Lorena Urbanelli
- CFU
- 2
- Course Regulation
- Coorte 2023
- Offered
- 2025/26
- Learning activities
- Altro
- Area
- Altre conoscenze utili per l'inserimento nel mondo del lavoro
- Academic discipline
- BIO/06
- Type of study-unit
- Obbligatorio (Required)
- Type of learning activities
- Attività formativa monodisciplinare
- Language of instruction
- Italian
- Contents
- Measurement of the protein concentration of an extract using the Bradford method and calibration line with a protein of known concentration Extraction, amplification and electrophoresis of nucleic acids Culture and counting of eukaryotic cells Extraction and measurement of total phenolic content from food matrix
- Reference texts
- Material provided by teachers.
- Educational objectives
- The course aims to provide students the ability to apply the knowledge acquired during each discipline lectures, such as understanding of the molecular basis of genetic transmission, familiarity with the use of some of the main methods applied in the biological chemistry, ability to relate cellular organization to functional.
- Prerequisites
- To benefit from the laboratory experiments on the topics listed in the program, students must have acquired basic knowledge on Genetics, Biological Chemistry, Anatomy.
- Teaching methods
- The course is organized in laboratory experiments regarding the following disciplines: Genetics, Biochemical Chemistry, Anatomy.
- Other information
- For the teaching calendar (lessons date, time and place), please check the Biological Sciences degree website in dcbb.unipg.it
- Learning verification modality
- To obtain the credits programmed for this teaching (2 credits-24 hours) it is necessary to attend at least 75% of the total number of hours, therefore at least 18 hours. The attendance will be assessed by roll call and signature capture. For information on support services for students with disabilities and / or DSA visit the page.
- Extended program
- GENETICS: Extraction, amplification and electrophoresis of nucleic acids for the analysis of gene and genomic sequences, and the study of intra- and inter-specific variability for phylogenetic and phylogeographic investigations. BIOLOGICAL CHEMISTRY: Determination by spectrophotometric method of the protein content of cellular extracts, including the preparation of the calibration curve. ANATOMY: Observation, culture and counting of different types of eukaryotic cells. Extraction and analysis of the contents of bioactive substances contained in cells and other matrices