Unit BIOCHEMICAL BIOTECHNOLOGY FOR MOLECULAR ASSAYS WITH LABORATORY

BIOCHEMICAL BIOTECHNOLOGY FOR MOLECULAR ASSAYS

Code	A004631
CFU	1
Teacher	Paolo Gorello
Teachers	Sabata Martino (Codocenza)
Hours	7 ore (Codocenza) - Sabata Martino
Learning activities	Affine/integrativa
Area	Attività formative affini o integrative
Academic discipline	BIO/10
Type of study-unit	Obbligatorio (Required)
Language of instruction	Italian
Contents	The course aims to provide students with knowledge of the main methods and technologies associated with biochemistry in the context of biotechnological research and in diagnostic and therapeutic applications. The course also includes a part of practical exercises on related topics (see (LABORATORIO PER LE BIOTECNOLOGIE BIOCHIMICHE PER SAGGI MOLECOLARI APPLICATI).
Reference texts	Mauro Maccarrone Biochemical and biomolecular methodologies Ed. Zanichelli. Teaching material provided by the teacher.
Educational objectives	At the end of the course the student will have acquired the basic theoretical knowledge of biochemical methods necessary to work effectively in a laboratory where relevant techniques are used.
Prerequisites	Basic knowledge of organic chemistry and biochemistry is required
Teaching methods	Frontal lectures. The course is organized to provide the theoretical contents that will then be addressed in the laboratory activities, which are described in the associated teaching (LABORATORY FOR BIOCHEMICAL BIOTHENOLOGIES FOR APPLIED MOLECULAR ASSAYS).
Other information	For teaching activities (dates and times of lectures, classroom), please refer to website dcbb.unipg.it and the course page on Unistudium.
Learning verification modality	Written exam. 30 multiple choice questions. For information on support services for students with disabilities and/or DSA visit the page http://www.unipg.it/disabilita-e-dsa
Extended program	Il programma del corso comprende esercitazioni pratiche riguardanti la preparazione di soluzioni tampone di interesse biochimico, con l’acquisizione di competenze riguardanti calcoli stechiometrici, diluzioni e percentuali; ii) l’utilizzo di sistemi tampone di interesse biologico e la misurazione del pH; l’estrazione di acidi nucleici e proteine mediante la separazione di fase; l’estrazione di sostanze lipidiche
Obiettivi Agenda 2030 per lo sviluppo sostenibile	Il corso fornisce abilità laboratoriali di base finalizzate al miglioramento delle competenze nei settori della salute e dell'ambiente

BIOCHEMICAL MOLECULAR DIGNOSTIC ASSAYS

Code	A004814
CFU	1
Teacher	Paolo Gorello
Teachers	Paolo Gorello
Hours	7 ore - Paolo Gorello
Learning activities	Affine/integrativa
Area	Attività formative affini o integrative
Academic discipline	BIO/12
Type of study-unit	Obbligatorio (Required)
Language of instruction	Italian
Contents	Selection of biological samples ( DNA, RNA and Proteins) and quality control analysis. PCR: Theoretical bases and set-up of PCR reactions. Database: nucleotide and amino acid sequences. Real-Time PCR: Theoretical bases, Fluorogenic probes, endogenous housekeeping genes. DNA sequencing and mutational analysis : Sanger method , NGS (Next Generation Sequencing) and Clinical applications.
Reference texts	Slides and notes provided by teacher
Educational objectives	The main aim of this teaching is to provide students with the theoretical principles that are the basis of the most common techniques of molecular biology, in order to identify possible applications in diagnosis.
Prerequisites	The course requires basic knowledge of Biochemistry, Cell Biology and Genetics.
Teaching methods	Lectures on all subjects of the couse.
Learning verification modality	Written Test
Extended program	Selection of biological samples ( DNA, RNA and Proteins) and quality control analysis. PCR: Theoretical bases and set-up of PCR reactions. Database: nucleotide and amino acid sequences. Real-Time PCR: Theoretical bases, Fluorogenic probes, endogenous housekeeping genes. DNA sequencing and mutational analysis : Sanger method , NGS (Next Generation Sequencing) and Clinical applications.
Obiettivi Agenda 2030 per lo sviluppo sostenibile	Good Health and well-being

LABORATORY OF BIOCHEMICAL BIOTECHNOLOGY FOR MOLECULAR ASSAYS IN DIAGNOSTICS

Code	A004776
CFU	2
Teacher	Sabata Martino
Teachers	Paolo Gorello (Codocenza)
Hours	24 ore (Codocenza) - Paolo Gorello
Learning activities	Altro
Area	Altre conoscenze utili per l'inserimento nel mondo del lavoro
Academic discipline	NN
Type of study-unit	Obbligatorio (Required)
Language of instruction	Italian
Contents	PCR, Electrophoresis, DATABASE
Reference texts	Slides and notes provided by teacher
Educational objectives	The main aim of this teaching is to provide students with the theoretical principles that are the basis of the most common techniques of molecular biology, in order to identify possible applications in diagnosis.
Prerequisites	The course requires basic knowledge of Biochemistry, Cell Biology and Genetics.
Teaching methods	Laboratory
Extended program	PCR, Electrophoresis, DATABASE
Obiettivi Agenda 2030 per lo sviluppo sostenibile	Good Health and well-being

LABORATORY FOR BIOCHEMICAL BIOTECHNOLOGIES FOR APPLIED MOLECULAR ASSAYS

Code	A004777
CFU	2
Teacher	Paolo Gorello
Teachers	Sabata Martino (Codocenza)
Hours	24 ore (Codocenza) - Sabata Martino
Learning activities	Altro
Area	Altre conoscenze utili per l'inserimento nel mondo del lavoro
Academic discipline	NN
Type of study-unit	Obbligatorio (Required)
Language of instruction	Italian
Contents	The course aims to provide students with knowledge of the main methods and technologies associated with biochemistry in the context of biotechnological research and in diagnostic and therapeutic applications. The course also includes a part of practical exercises on related topics (see BIOCHEMICAL BIOTECHNOLOGIES FOR APPLIED MOLECULAR ASSAYS)
Reference texts	Mauro Maccarrone Biochemical and biomolecular methodologies Ed. Zanichelli. Teaching material provided by the teacher.
Educational objectives	At the end of the course the student will have acquired the theoretical and practical knowledge of biochemical methods necessary to work effectively in a laboratory where relevant techniques are used.
Prerequisites	Basic knowledge of Organic Chemistry and Biochemistry
Teaching methods	l practical lessons in the teaching laboratory relating to the topics that are provided in the course of BIOCHEMICAL BIOTECHNOLOGIES FOR MOLECULAR ASSAYS.
Other information	For teaching activities (dates and times of lectures, classroom), please refer to website dcbb.unipg.it and the course page on Unistudium
Learning verification modality	The course provides basic laboratory skills aimed at improving skills in the health and environmental sectors.
Extended program	The course program includes practical exercises regarding the preparation of buffer solutions of biochemical interest, with the acquisition of skills regarding stoichiometric calculations, dilutions and percentages; ii) the use of buffer systems of biological interest and the measurement of pH; preparation of protein extracts or nucleic acids Determination of proteins and nucleic acids. Enzyme assays. PCR. ELISA assays. Use of specialized instrumentation for the determination of proteins and nucleic acids.
Obiettivi Agenda 2030 per lo sviluppo sostenibile	The course provides basic laboratory skills aimed at improving skills in the health and environmental sectors.