Unit ELEMENTS OF GENERAL MICROBIOLOGY AND APPLIED GENETICS WITH LABORATORY

ELEMENTS OF APPLIED GENETICS ELEMENTS OF APPLIED GENETICS

Code	A004622
CFU	2
Teacher	Hovirag Lancioni
Teachers	Hovirag Lancioni
Hours	14 ore - Hovirag Lancioni
Learning activities	Affine/integrativa
Area	Attività formative affini o integrative
Academic discipline	BIO/18
Type of study-unit	Obbligatorio (Required)
Language of instruction	Italian
Contents	Molecular Genetics
Reference texts	Russel Genetics - An integrated approach. Pearson
Educational objectives	Biotechnological skills - Knowledge and understanding of the structure and biological properties of viruses and bacteria, including genetically modified ones; of the general mechanisms of the transmission of hereditary characters; the molecular basis of transmission and expression of gene information; of molecular analysis methodologies; Ability to apply knowledge and consumption of mathematical, physical, statistical and computer models for analysis and biological processes; Knowledge and methodologies for the genetic modification of cells and animals.
Prerequisites	Cytology
Teaching methods	Frontal lessons
Other information	Frequency: Optional but recommended
Learning verification modality	Final Oral Exam: the evaluation consists of an oral exam, which will serve to ascertain the level of knowledge and ability to explain the topics covered during the course. Students with disabilities and/or DSA are invited to visit the page dedicated to the tools and measures envisaged and to agree in advance what is necessary with the teacher (https://www.unipg.it/disabilita-e -dsa)
Extended program	DNA analyses methods. Enzymes for DNA manipulation: DNA polymerase, nuclease, ligase. Methods for studying the molecular variability of human mtDNA: from RFLP analysis to whole genome sequencing. DNA sequencing: classic automated method. Evolutionary models and molecular phylogeny. Molecular markers: classification. Physical mapping (restriction maps, FISH, and STS mapping). Correlation between genetic and physical mapping. Traditional genome sequencing via hierarchical and shotgun approaches. Gene libraries: cloning vectors. Next generation DNA sequencing (e.g. Illumina sequencing). Analysis of genomic sequences. Genetic tests. Examples of applied genetics.
Obiettivi Agenda 2030 per lo sviluppo sostenibile	Health and wellness Life on earth

ELEMENTS OF GENERAL MICROBIOLOGY

Code	A004621
CFU	2
Teacher	Ermanno Federici
Teachers	Ermanno Federici
Hours	14 ore - Ermanno Federici
Learning activities	Affine/integrativa
Area	Attività formative affini o integrative
Academic discipline	BIO/19
Type of study-unit	Obbligatorio (Required)
Language of instruction	Italian
Contents	Basic principles of the biology of microorganisms. Structures and functions of bacteria.
Reference texts	Madigan e Martinko - "Brock, Biologia dei microrganismi"
Educational objectives	Knowledge of the main structure and function of bacteria
Prerequisites	n.a.
Teaching methods	Lesons
Other information	Course venue: see Department website (http://www.dcbb.unipg.it) Contact the Professor for a meeting
Learning verification modality	Colloquium
Extended program	Biodiversity of microorganisms. Cellular and molecular organization of prokaryotic microorganisms. Structure and ultrastructure of the bacterial cell. Cytoplasm and genome; Cytoplasmic membrane (structure and transport mechanisms). Cell wall: composition and structure of peptidoglycan in gram-positive and gram-negative bacteria and parietal membrane of gram-negative bacteria; Spores. Metabolic diversity. Bacterial growth

LABORATORY OF APPLIED GENETICS

Code	A004769
CFU	2
Teacher	Hovirag Lancioni
Teachers	Hovirag Lancioni
Hours	24 ore - Hovirag Lancioni
Learning activities	Altro
Area	Altre conoscenze utili per l'inserimento nel mondo del lavoro
Academic discipline	NN
Type of study-unit	Obbligatorio (Required)
Language of instruction	Italian
Contents	Molecular Genetics
Reference texts	Russel Genetics - An integrated approach. Pearson
Educational objectives	Biotechnological skills - Knowledge and understanding of the structure and biological properties of viruses and bacteria, including genetically modified ones; of the general mechanisms of the transmission of hereditary characters; the molecular basis of transmission and expression of gene information; of molecular analysis methodologies; Ability to apply knowledge and consumption of mathematical, physical, statistical and computer models for analysis and biological processes; Knowledge and methodologies for the genetic modification of cells and animals.
Prerequisites	Cytology and genetics
Teaching methods	Frontal lessons and lab activity
Other information	Frequency: Optional but recommended
Learning verification modality	Final Oral Exam: the evaluation consists of an oral exam, which will serve to ascertain the level of knowledge and ability to explain the topics covered during the course. Students with disabilities and/or DSA are invited to visit the page dedicated to the tools and measures envisaged and to agree in advance what is necessary with the teacher (https://www.unipg.it/disabilita-e -dsa)
Extended program	Laboratory activities: DNA extraction, PCR amplification, sequencing of the control region of mitochondrial DNA, molecular identification of sex, restriction analysis, classification of sequences into haplogroups and insertion into a phylogenetic tree. Applications: mitochondrial DNA and phylogenetic analysis; Application of the study of genomes in medical genetics, the example in hemoglobinopathies. Telomere structure, and alterations in telomere length: methods used for the analysis.
Obiettivi Agenda 2030 per lo sviluppo sostenibile	Health and wellness Life on earth

MICROBIOLOGY LABORATORY

Code	A000806
CFU	3
Teacher	Ermanno Federici
Teachers	Ermanno Federici
Hours	36 ore - Ermanno Federici
Learning activities	Altro
Area	Altre conoscenze utili per l'inserimento nel mondo del lavoro
Academic discipline	NN
Type of study-unit	Obbligatorio (Required)
Language of instruction	Italian
Contents	Methods used in the microbiology laboratory. Culture and molecular techniques for determining the quantity of bacteria and for the identification of bacterial isolates
Reference texts	n.a.
Educational objectives	Introducing the student to the main techniques used in the microbiology laboratory
Prerequisites	none
Teaching methods	Lab experiences
Other information	Course venue: see Department website (http://www.dcbb.unipg.it) Contact the Professor for a meeting
Learning verification modality	Colloquium. The examiner will check the student’s level of knowledge and ability to build connections between various parts of the Microbiology program, as well as terminology used and command of technical language
Extended program	Cultivation and isolation of microorganisms in the laboratory. Culture media and their classification; Aseptic technique; Viable plate counts; Methods for isolating pure cultures; Bacterial identification; Molecular techniques: DNA extraction; qPCR, sequencing