Unit ADVANCED MOLECULAR BIOLOGY

Course
Pharmaceutical biotechnologies
Study-unit Code
GP003556
Curriculum
In all curricula
Teacher
Elisabetta Albi
Teachers
  • Elisabetta Albi
  • Carmela Conte (Codocenza)
Hours
  • 26 ore - Elisabetta Albi
  • 26 ore (Codocenza) - Carmela Conte
CFU
6
Course Regulation
Coorte 2025
Offered
2025/26
Learning activities
Caratterizzante
Area
Discipline biotecnologiche comuni
Academic discipline
BIO/11
Type of study-unit
Obbligatorio (Required)
Type of learning activities
Attività formativa monodisciplinare
Language of instruction
Italian
Contents
From gene to the postgenomic era
Reference texts
Biologia Molecolare
Amaldi-Benedetti-Pesole-Plevani
Casa editrice A,brosiana
Educational objectives
The aim will be to provide the specific notions about gene expression and advanced methodologies.
Prerequisites
Basis of Molecular biology
Teaching methods
Frontal lesson and practical class
Learning verification modality
Oral exam
Extended program
NUCLEIC ACIDS STRUCTURES
DNA DUPLICATION
DNA TRANSCRIPTION
DNA TRANSLATION
CLONING VECTORS – CONSTRUCTION AND ANALYSIS OF cDNA, GENOMIC, LINKING AND JUMPING LIBRAIES -STUDYING GENE LOCATION AND STRUCTURE – STUDYING GENE EXPRESSION AND FUNCTION - STUDYING GENOMES - SITE-DIRECETED MUTAGENESIS OF CLONED DNA - SNP - TWO HYBRID SYSTEM – REAL-TIME PCR - SAGE – MICROARRAY – PROMOTER ANALYSIS – 5’RACE – 3’ RACE –
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