Unit HUMAN ISTOLOGY AND EMBRIOLOGY

HUMAN EMBRIOLOGY

Code	GP001285
CFU	3
Teacher	Giovanni Luca
Teachers	Giovanni Luca
Hours	37.5 ore - Giovanni Luca
Learning activities	Base
Area	B_03. morfologia umana
Sector	BIO/17
Type of study-unit	Obbligatorio (Required)

HUMAN ISTOLOGY

Code	A004710
CFU	5
Teacher	Giovanni Luca
Teachers	Lorella Marinucci (Codocenza) Giovanni Luca
Hours	12.5 ore (Codocenza) - Lorella Marinucci 50 ore - Giovanni Luca
Learning activities	Base
Area	B_03. morfologia umana
Sector	BIO/17
Type of study-unit	Obbligatorio (Required)
Language of instruction	Italian
Contents	Optical microscopy. The preparation of tissue. Electron microscopy. Resolving power. The examination and interpretation of sections. Stains. GENERAL HISTOLOGY HUMAN (with notes of special and functional histology) 1) Definition and classification of tissues; 2) Tissues: epithelial, connective tissue proper, specialized connective (cartilage, bone and blood), adipose, nervous and muscular.
Reference texts	Histology atlases racommended:- Atlante di Istologia e Anatomia Microscopica, Ross et al., Casa Editrice Ambrosiana- Istologia Funzionale, Kerr, Casa Editrice Ambrosiana.- Bani D., Bani G., Bani Sacchi T. - Istologia - Casa Editrice Idelson-GnocchiImages of microscopic slides are also available in different websites, such as: http://web.unife.it/cdl/istomed/photo.htm. Testo di consultazione e con analisi dei singoli organi: - M.H. Ross & W.Pawlina, Istologia - Testo e atlante con elementi di biologia cellulare e molecolare,edizione italiana CEA 2016.
Educational objectives	At the end of the course the student must: A) own autonomy in the use of the microscope; B) have acquired knowledge on the main histological techniques used in the laboratory; C) have acquired the ability to recognize critically the various tissues in histological preparations observed at the optical microscope. The student must acquire adequate knowledge on biomorphological structure and function of the human body tissues
Prerequisites	In order to be able to understand and apply the majority of the contents described within the course, the student must have acquired basic knowledge of cellular biology.
Teaching methods	Lectures . Projections of histological preparations in the classroom with an indication of tissue recognition strategies.
Other information	Frequency: compulsory. University campus: Street Tristano di Joannuccio (Hospital S. Maria) - 05100 Terni.
Learning verification modality	The final exam consists in a recognition test of histological preparations observed on texts or under an optical microscope.
Extended program	MEANS AND METHODS OF INVESTIGATION. Brief history of Histology and its methods of study: the first means of investigations until microscope light. The optical microscope: the 'parts' of the optical microscope. Limits of light microscope. Resolving power. Notes on the fluorescence microscope and the microscope in phase contrast. The electron microscope: the basic structure. Theoretical resolving power and real resolving power. The scanning microscope. Technical set-up of the slides: fixation the inclusion. Freezing-drying. Artefacts. Dyes: classification and properties. Technical construction of the sections: microtome, ultramicrotome, cryostat. Signs on histochemical methods: localization of proteins, lipids and glucides. Strategies for recognition by light microscopy about epithelial tissue, glandular exocrine and endocrine epithelial; connective, cartilage, bone and blood; muscle tissue and nerve tissue.
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