Unit FUNCTIONAL BIOLOGY
- Course
- Molecular and industrial biotechnology
- Study-unit Code
- 55A01706
- Curriculum
- In all curricula
- Teacher
- Maria Laura Belladonna
- Teachers
-
- Maria Laura Belladonna
- Hours
- 57 ore - Maria Laura Belladonna
- CFU
- 6
- Course Regulation
- Coorte 2020
- Offered
- 2021/22
- Learning activities
- Caratterizzante
- Area
- Discipline biologiche
- Academic discipline
- BIO/13
- Type of study-unit
- Obbligatorio (Required)
- Type of learning activities
- Attività formativa monodisciplinare
- Language of instruction
Italian- Contents
Pathogens and infection. Innate and adaptive immune system. Cell death. Cancer.
Laboratory: cloning of a gene construct and its expression in eukaryotic cells.- Reference texts
Alberts et al. “ BIOLOGIA MOLECOLARE DELLA CELLULA” Ed. Zanichelli – 6th edition- Educational objectives
The main objective of the course of Functional Biology is the integrated study of an animal cell, with particular regard to mechanisms involved in immune response, programmed cell death, and cancer development. Students should acquire the ability to connect different cellular processes to both basic and specializing biological knowledge.- Prerequisites
To effectively follow and understand the topics covered in the course of Functional Biology is necessary for the student to have a basic knowledge of animal cell biology as it is normally acquired in basic programs of biological and/or biotechnological degree courses.- Teaching methods
Face-to-face lessons and laboratory- Other information
For information on support services for students with disabilities and / or DSA visit the page
http://www.unipg.it/disabilita-e-dsa- Learning verification modality
The exam consists of an oral examination not longer than 30 minutes and enables to verify of the level of knowledge and understanding achieved by the student; the ability of the student to highlight the correlations between the various biological processes. In addition, the oral examination will assess the student's ability to communicate and expose with appropriate language the topics of cell biology, with particular regard to those discussed during face-to-face lessons and lab activities. The exam will be performed at the end of the course according to the exam timetable. The evaluation will be in thirtieths (minimum: 18/30; maximum: 30/30 cum laude).- Extended program
Pathogens and infection: fundamentals of pathogens and human microbiota; cellular biology of infection. Innate and adaptive immune system: cellular and molecular mechanisms of the innate immune system (epithelial barrier, PRR receptors, inflammation and cytokines, phagocytic cells, complement system, dendritic cells); cellular and molecular mechanisms of the adaptive immune system (immunization, MHC proteins, antigens, immunoglobulins, T e B lymphocytes, lymphocyte clonal selection, immunological memory, immunological tolerance, costimulatory signals). Cellular death: fundamentals and molecular mechanisms of apoptosis extrinsic and intrinsic pathways; apoptosis regulation (Bcl-2, IAP, etc.).Cancer: development and progression mechanisms of tumors; crucial genes for cancer (oncogenes and oncosuppressor genes, retroviral genes, Ras, Rb, p53), cancer prevention e treatment.
Laboratory: cloning of a gene construct into an expression vector, eukaryotic cell transfection, and analysis of expressed protein.