Unit TRANSLATIONAL MOLECOLAR BIOTECHNOLOGY
- Course
- Molecular and industrial biotechnology
- Study-unit Code
- 55A01812
- Curriculum
- In all curricula
- Teacher
- Francesco Grignani
- CFU
- 12
- Course Regulation
- Coorte 2020
- Offered
- 2021/22
- Type of study-unit
- Obbligatorio (Required)
- Type of learning activities
- Attività formativa integrata
CELLULAR AND TISSUE BIOTECHNOLOGY
| Code | 55A01906 |
|---|---|
| CFU | 6 |
| Teacher | Mario Calvitti |
| Teachers |
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| Hours |
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| Learning activities | Affine/integrativa |
| Area | Attività formative affini o integrative |
| Academic discipline | BIO/17 |
| Type of study-unit | Obbligatorio (Required) |
| Language of instruction | Italian |
| Contents | Morphological features of human normal tissues. Staining methods - Histologic and Histochemical. Cell culture. |
| Reference texts | - Istologia per le Professioni Sanitarie, Sica et al. Casa Editrice Idelson-Gnocchi, 2015.- M. Melis-F. Carpino-U. Di Tondo. Tecniche in Anatomia Patologica - edi-ermes.- E. Bonucci – “Manuale di Istochimica”- Edi-Lombardo.- Anatomia Patologica – Le basi – Luigi Ruco, Aldo Scarpa – UTET |
| Educational objectives | At the end of the course, student must reach the critical knowledge of: A) morphological features of human normal tissues. B) Staining methods - Histologic and Histochemical C) Cell culture. |
| Prerequisites | In order to be able to understand and apply the majority of the contents described within the course, the student must have acquired basic knowledge of cellular biology. |
| Teaching methods | Face-to-face. Practical training Theoretical lessons and practical training Laboratory. |
| Other information | Recommend the attendance. |
| Learning verification modality | At the end of the course, student must reach the critical knowledge of: A) morphological features of human normal tissues. B) Staining methods - Histologic and Histochemical C) Cell culture. |
| Extended program | Course of Histology. Lining epithelium: general considerations, classification and main examples. Exocrine and endocrine glands: classification and examples. Connective tissues and adipose tissue. Cartilage. Bone tissue. Bone development. Blood (plasma; erythrocytes, granulocytes, lymphocytes, monocytes, platelets). Muscular tissues: morphology of smooth and striated muscles. Molecular mechanism of contraction. Nervous tissue: general organization. Neurons and neuroglial cells. Nerve fibers, peripheral nerves. Synapses: morpho-functional organization. Staining methods - Histologic and Histochemical. Tissue fixation. Aldehyde fixative. Formalin. Glutaraldehyde. Formaldehyde. Bouin's fluid. Osmium tetroxide as fixative. Decalcification of bone. Dehydration. Paraffin embedding. Tissue Sectioning. Microtome with paraffin section. Cold microtome (cryostat) methods. Microtome with frozen section. Frozen section routines. Paraffin section methods. Deparaffinization. Treatment of section before and after staining. Dehydration of paraffin section. Mounting of sections. Ematoxylin and Eosin for paraffin section. Azan-Mallory. Alcian blu-haematoxylin staining. Alcian blue 8GX for acid mucopolysaccharides. The Periodic Acid-Schiff Reaction (PAS). PAS- haematoxylin staining. Alcian blue-PAS stain. Toluidine blue stains for metachromasia. Resorcin fuchsin stain of Weigert. Weigert for elastic fiber stain. Von Kossa method for phosphates and carbonates. Silver impregnation method for reticulum fibers. Sudan III. Immunofluorescence. Electron microscopy. Cell culture. Isolation of cells. Maintaining cells in culture. Manipulation of cultured cells. Media changes. Passaging cells. Applications of cell culture. |
IMMUNOLOGICAL BIOTECHNOLOGY
| Code | 55A02006 |
|---|---|
| CFU | 6 |
| Teacher | Francesco Grignani |
| Teachers |
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| Hours |
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| Learning activities | Caratterizzante |
| Area | Discipline per le competenze professionali |
| Academic discipline | MED/04 |
| Type of study-unit | Obbligatorio (Required) |
| Language of instruction | Italian |
| Contents | Biotechnologies using immunological tools in the study, diagnosis and therapy of human pathology, with a selection of the most advanced immunological, cellular and molecular tools. |
| Reference texts | Janeway's Immunobiology Garland Science Abbas, Lichtman, Pillai: Cellular and Molecular Immunology |
| Educational objectives | Basic knowledge of the immune system and the main immunological approaches in the biotechnological sector. In particular, knowledge of classic and modern polyclonal and monoclonal antibody production strategies; on the diagnostic and therapeutic applications of antibodies in oncology, rheumatology and cardiology; on the fundamental immunochemical and immunofluorescence techniques, including the flow cytometer; on the main applications in research of Immunoprecipitation technologies for the analysis of proteins, RNA, and chromatin. |
| Prerequisites | Cell biology and signaling transduction. Innate and specific immune system cells. Basic mechanisms of immune reactivity. |
| Teaching methods | Lectures, class discussions, visualization and commentary of images and laboratory procedures. Viewing movies. Work in a group. Inverted classes. Peer to peer teaching |
| Learning verification modality | Classroom discussion. Presentation of specific topics and insights and Bibliographic research, as oral exam constituents |
| Extended program | 2576/5000 - Basic immunology: innate and acquired immunity. Antigens and antigenic recognition by B and T lymphocytes. The antibody response. I-IV hypersensitivity reactions such as allergies and diagnostic tests. - Structure and function of antibodies (Ab) or immunoglobulins (Ig). - Protein immunoprecipitation: principles and uses. - Immunological assays and methods based on the antigen-antibody reaction: precipitation (in solution and agarose gel) and agglutination. Some applications in the biomedical diagnostic field: blood typing, bacterial typing, pregnancy test, Coombs test. Nephelometry, Turbidimetry - Polyclonal antibodies: production of an antiserum and its applications. - Monoclonal antibodies (mAb): production of murine, chimeric, humanized, human mAb (using phage display technology, transgenic animals and other technologies). - Applications of mAb in the diagnostic and therapeutic biomedical field. - Mechanisms of therapeutic antibody activity - Bispecific antibodies, BITEs and Single Chain Variable Fragments (scFv) - Chimeric antigenic receptors (CARs) - Antibodies used in anti-inflammatory therapy and in immune pathologies - Antibodies used in cholesterol lowering therapy - Immune response to tumors and tumor immunotherapy. - Signal transduction of growth factors and main types of receptors. - Antibodies active on proliferative and angiogenetic signaling - Antibodies useful for activating anti-tumor immunity (CTLA-4 system, PD-1 PDL, others) - Cellular immunotherapy and CAR-Ts - Immunotherapeutic combinations. - Chromatin structure and epigenetic regulation of gene expression - Chromatin Immunoprecipitation (ChIP) - ChIP Sequencing and ChIP on chip - RNA immunoprecipitation (RIP) - RIP-Sequencing - Direct and indirect immunofluorescence techniques for the identification of surface and intracellular antigens. - Flow cytometry. Its applications in the analysis of the normal and pathological white blood cell phenotype. Elements of cell sorting. - Radio Immunological Assays (RIA, radio immuno-assay) and Immuno-Enzyme Adsorption ELISA (enzyme-linked immunosorbent assay), for the identification and dosage of antigens and antibodies. Some applications in the biomedical diagnostic field - Transplants and transplant rejection: tissue typing and donor-recipient compatibility tests. - Immune response to infections and strategies for vaccine development. |