Unit BREEDING BIOTECHNOLOGIES AND LABORATORY ANIMALS

Course
Medical, veterinary and forensic biotechnological sciences
Study-unit Code
A001782
Curriculum
Veterinario
Teacher
Andrea Verini Supplizi
CFU
12
Course Regulation
Coorte 2019
Offered
2020/21
Type of study-unit
Obbligatorio (Required)
Type of learning activities
Attività formativa integrata

BIOTECHNOLOGY APPLIED TO ANIMAL BREEDING

Code A001784
CFU 6
Teacher Andrea Verini Supplizi
Teachers
  • Andrea Verini Supplizi
Hours
  • 52 ore - Andrea Verini Supplizi
Learning activities Affine/integrativa
Area Attività formative affini o integrative
Academic discipline AGR/19
Type of study-unit Obbligatorio (Required)
Language of instruction English
Contents Principal biotechnologies applied to animal breeding, including procedures used for genetic improvement of farm animals.
Reference texts Genetics and genomics 3, Barcaccia Falcinelli, Ed Liquors 2007

available

handouts lessons, online material,
Educational objectives The student has to acquire basic knowledge on the principal biotechnologies applied to animal production including aquatic organism. Moreover, the impact of biotechnology on animal weklfare, animal breeding and food safety

The main skills are:

DNA extraction up to the execution and interpretation of PCR

Recovery and evaluation of female gametes

These skills will be acquired and evaluated during laboratory exercises
Prerequisites
Teaching methods Face-to-face
Laboratory
Other information
Learning verification modality
Extended program Introduction to biotechnology
Utilisation of biotechnologies and Environmental impact
Reproductive Biotechnologies
MOET
Cryoconservation of gametes
Embryo Sexing
Sperm Sexing
IVM
IVF- IVC
Reproductive Cloning by blastomere and somatic cells
Non reproductive cloning by somatic and stem cells
Transgenic animals: transfer of gene construct:
Transgenic animals: applications in animal production

LABORATORY ANIMALS AND ANIMAL EXPERIMENTATION

Code A001783
CFU 6
Teacher Paolo Mosci
Teachers
  • Paolo Mosci
Hours
  • 52 ore - Paolo Mosci
Learning activities Affine/integrativa
Area Attività formative affini o integrative
Academic discipline VET/08
Type of study-unit Obbligatorio (Required)
Language of instruction Italian
Contents The course provides notions about the possibility and the usefulness of activating research projects using laboratory animals and the current legal provisions concerning.The ethology and biology of the most common species of laboratory animals, breeding techniques, pathologies, their genetic and microbiological classification. Are therefore treated the basic experimental procedures and manipulations, the conditions of animal welfare and the setting of the experimental design are also illustrated.
Reference texts priciples of laboratory animals science.(LFM.van Zupten) Goliardica Pavese
The
experimental Animal in Biomedical Research vol II (B.E. Rollin) CRC PRESS
Educational objectives Knowledge and understanding.The student must know the biological and ethological basis of the species of experimental interest, the techniques of management and housing of animals, the basic techniques and procedures for the activation of experimental protocols, the methods of assessment of the state of well-being, The criteria for the scientific design of an experimental design.
Prerequisites In order to understand the topics treated, the knowledge of Ethology, Animal Anatomy and Physiology and General Pathology are useful
Teaching methods lectures and laboratory activities in the laboratories of the Animal Facility of Università degli Studi di Perugia
Other information
Learning verification modality final test
Extended program 1.Animal experimentation: validity and ethical aspects.
2.Legislation concerning animal experimentation
3. Laboratory animal biology: mouse,rat,hamster,gerbil,guinea pig, rabbit
4. Breeding and housing techniques. 5. overview of pahtology of laboratory animals.
6. Genetic classification of laboratory animals.
7. Microbiological classificationof laboratory animals.
8. Animal welfare, 3R, Hhumane endpoint.
9 Priciples of laboratory animal anesthesia. 10. Basic experimental procedures and manual skills.
11. Elements of embryonic manipolation.ù 12. experimemntal design
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