Unit IN VITRO CULTURE
- Course
- Agricultural and environmental sciences
- Study-unit Code
- 80228606
- Curriculum
- Biotecnologie
- Teacher
- Maurizio Micheli
- Teachers
-
- Maurizio Micheli
- Hours
- 54 ore - Maurizio Micheli
- CFU
- 6
- Course Regulation
- Coorte 2019
- Offered
- 2021/22
- Learning activities
- Caratterizzante
- Area
- Discipline della produzione vegetale
- Academic discipline
- AGR/03
- Type of study-unit
- Obbligatorio (Required)
- Type of learning activities
- Attività formativa monodisciplinare
- Language of instruction
- Italian
- Contents
- In vitro culture laboratory: characteristics and organization. Main equipment. Basics on the gamic and agamic reproduction of arboreal, shrubby and herbaceous species of agricultural, ornamental and spontaneous interest. Definition of in vitro culture. In vitro regeneration methods and their application. Safeguarding of ex situ biodiversity. Morphological, physiological and biochemical bases of in vitro morphogenesis. Cellular totipotency. In vitro micropropagation and cloning; causes of somaclonal mutations and their use. Mother plants: choice and removal of the plant material in relation to the phenological phase and the intrinsic characteristics of each plant species. Phases of micropropagation and specificity of substrates and their components with reference to the individual species to be cultivated: substrates of stabilization, proliferation, rooting and acclimatization. Choice of explants. Application possibilities of micro-propagation in broad-leaved trees, conifers, bulbous species and aquatic plants. Ex situ conservation of plant germplasm. Each process and phase of in vitro culture will be supported by practical laboratory activities such as: handling in aseptic conditions, culture substrates, removal of meristematic apices, explant stabilization, verification of the in vitro proliferation coefficient obtainable from different types of in vitro explants -Currency derivatives. Implementation of in vitro and in vivo rooting procedures for micropropagated material. Acclimatization and return in situ of the various plant species.
- Reference texts
- - "MICROPROPAGAZIONE DELLE PIANTE ORTOFLOROFRUTTICOLE". G. De Paoli, V. Rossi, A. - responsabile.Scozzoli - EDAGRICOLE-Edizioni Agricole della Calderini s.r.l. - Bologna, 1994.
- "VEGETATIVE PROPAGATION. VITRO CULTURE OF HIGHER PLANTS". Pierik R.L.M. - Martinus Nijhoff Publisher -Dordrecht- TheNetherlands, 1987.
- "Mezzi e tecniche per la coltura in vitro dei vegetali - Speciale micropropagazione: notiziario tecnico" ERSO Ente per la Ricerca la Sperimentazione la divulgazione in Ortofrutticoltura, floricoltura e sementi . - Educational objectives
- Acquire knowledge on: techniques related to micropropagation and in vitro regeneration processes (embryogenesis and organogenesis) also through laboratory practices;To know the application possibilities of in vitro culture techniques for conservation, diffusion of plant germplasm, genetic improvement and production of useful substances. The student should acquire the ability to apply the techniques of manipulation in asepsis of plant explants.
- Prerequisites
- Having passed the examination Arbpricoltura generale e tecnica vivaistica
- Teaching methods
- Frontal lessons; laboratory practices.
- Other information
- Recommended frequency.
For information on support services for students with disabilities and/or DSA visit the link:
www.unipg.it/disabilita-e-dsa - Learning verification modality
- Oral examination
- Extended program
- In vitro culture laboratory: characteristics and organization. Main equipment. Basics on the gamic and agamic reproduction of arboreal, shrubby and herbaceous species of agricultural, ornamental and spontaneous interest. Definition of in vitro culture. In vitro regeneration methods and their application. Safeguarding of ex situ biodiversity. Morphological, physiological and biochemical bases of in vitro morphogenesis. Cellular totipotency. In vitro micropropagation and cloning; causes of somaclonal mutations and their use. Mother plants: choice and removal of the plant material in relation to the phenological phase and the intrinsic characteristics of each plant species. Phases of micropropagation and specificity of substrates and their components with reference to the individual species to be cultivated: substrates of stabilization, proliferation, rooting and acclimatization. Choice of explants. Application possibilities of micro-propagation in broad-leaved trees, conifers, bulbous species and aquatic plants. Ex situ conservation of plant germplasm. Each process and phase of in vitro culture will be supported by practical laboratory activities such as: handling in aseptic conditions, culture substrates, removal of meristematic apices, explant stabilization, verification of the in vitro proliferation coefficient obtainable from different types of in vitro explants -Currency derivatives. Implementation of in vitro and in vivo rooting procedures for micropropagated material. Acclimatization and return in situ of the various plant species.