Unit METHODOLOGIES OF CITOLOGICAL AND HISTOLOGICAL SURVEY
- Course
- Biology
- Study-unit Code
- A001032
- Curriculum
- Biosanitario
- Teacher
- Ines Di Rosa
- Teachers
-
- Ines Di Rosa
- Hours
- 47 ore - Ines Di Rosa
- CFU
- 6
- Course Regulation
- Coorte 2020
- Offered
- 2020/21
- Learning activities
- Caratterizzante
- Area
- Discipline del settore biodiversità e ambiente
- Academic discipline
- BIO/06
- Type of study-unit
- Obbligatorio (Required)
- Type of learning activities
- Attività formativa monodisciplinare
- Language of instruction
- Italian
- Contents
- Instruments for the cytological and histological analyses: light and electron microscopy. Principles and methods of the biological sample preparation. Histomorphological stains. Technique for identifying the cellular or tissue constituents in situ: histochemical, histoenzymatic and immunohistochemical stains. In situ hybridization. Cytology diagnostics. Histopathology.
- Reference texts
- Equipements provided by the teaching.
- Educational objectives
- The main knowledge acquired will be:
- principles and applications of instruments for cytological and histological analyses;
- basic and advanced methods of cytological and histological analyses.
The main competence will be:
- ability to apply instruments for cytological and histological analyses;
- ability to apply methods for cytological and histological analyses on scientific, biomedical and environmental investigations;
- ability to assess the results of cytological and histological analyses on scientific, biomedical and environmental investigations. - Prerequisites
- In order to be able to understand the topics of the program, students must have acquired basic knowledge of Cytology, Histology, Anatomy, Molecular Biology.
- Teaching methods
- The course is organized as follows:
-lectures on all topics listed in the program;
-laboratory on some topics listed in the program. Students will follow 3 tutorials of 4 hours each. - Learning verification modality
- The exam includes an oral test consisting of an interview of about 40 minutes long aiming to ascertain the knowledge level and understanding
capability acquired by the student about the topics discussed during the course. The oral exam is also intended to test student synthesis capability and propriety of language.
For information on support services for students with disabilities and / or DSA visit the page. - Extended program
- Instruments for the cytological and histological analyses: bright field microscope, dark field microscope, phase contrast microscope, fluorescence microscope, confocal microscope, multiphoton microscope, super resolution fluorescence microscope, transmission and scanning electron microscopes.
Methods of preparation of cytological and histological specimens: fresh specimens, fixed specimens (sampling, chemical and physical fixation, embedding in paraffin wax, epoxy and acrylic resins, sectioning, chemistry of staining, common laboratory stains).
Histomorphological, cytochemical, histochemical, histoenzymatic and immunohistochemical stains. In situ hybridization.
Cytology diagnostics: material collection; slide preparation; sample fixation; staining of the cytology smears; smears examination.
Histopathology: histopathological analyse in diagnosis of infectious diseases and in environmental studies.