Unit APPLIED CLINICAL BIOCHEMISTRY

Course
Biology
Study-unit Code
A001033
Curriculum
Biosanitario
Teacher
Luca Avellini
Teachers
  • Luca Avellini
  • Paolo Gorello
Hours
  • 42 ore - Luca Avellini
  • 10 ore - Paolo Gorello
CFU
6
Course Regulation
Coorte 2021
Offered
2021/22
Learning activities
Caratterizzante
Area
Discipline del settore biomedico
Academic discipline
BIO/12
Type of study-unit
Obbligatorio (Required)
Type of learning activities
Attività formativa monodisciplinare
Language of instruction
Italian
Contents
Knowledge necessary to understand the process of production of laboratory data for its critical interpretation. Basic concepts of analytical variability and biological variation, pre-analytical phase, reference values and quality control. Basic concepts of the most used techniques. Point-of-Care testing; Biochemical markers of function or damage of tissues or organs in different animal species. In particular: glucose homeostasis, dynamic function tests, transmissible spongiform encephalopathies.
Description of the most used techniques in Clinical Biochemistry: Nucleic acids electrophoresis, Protein electrophoresis, Western-blotting , ELISA assay, RIA assay, Fluorescence Microscopy.- Basic elements of Molecular Biology (genome, gene, mutations, polymorphisms) and utilization of databases (eg. NCBI, Enseble Genome Browser, Globin Gene Server).- Integration between Clinical Biochemistry methods and Molecular Biology methods : Study of Hemoglobinopathies- Description of the most used techniques in Molecular Diagnostics:end-point PCR , Real-Time PCR, dHPLC, DNA sequencing (Sanger method) , NGS (Next Generation Sequencing).
Reference texts
G. Federici - MEDICINA DI LABORATORIO - McGraw-Hill
A. Gaw, M.J. Murphy, R.A. Cowan - Biochimica clinica – Elsevier
Other tests will be comunicated by the professors at the beginning of the course
On-line availability (Unistudium) of the material used by the teacher for lessons
Educational objectives
The main aim of the teaching is to provide students with the theoretical principles needed to understand the applications and issues that underlie the methods most used in Clinical Biochemistry and Molecular Biology.
KNOWLEDGE AND UNDERSTANDING
The student must have knowledge of:
- the interpretation ability of clinical laboratory data
- the meaning and limits of clinical laboratory data;
- collection, preservation and transport of biological samples;
APPLYING KNOWLEDGE AND UNDERSTANDING
At the end of the course the student must have developed the following skills:
- to use the simplest analytical instruments and to apply basic knowledge of common methods used in Clinical Biochemistry;
- select appropriate diagnostic tests based on its analytical quality.
AUTONOMY OF JUDGMENT
The student must be able to:
- Communicate clearly and collaborate with referral and diagnostic services;
- interpret and understand the limitations of the test results.
COMMUNICATION SKILLS
At the end of the course, the student must be able to organize and expose the acquired knowledge supporting them with argumentative rigor, completeness, language properties and connection skills into other contexts both in written and oral form.
LIFELONG LEARNING SKILLS
At the end of the course the student will be able to:
- integrate autonomously the acquired knowledge by consulting and understanding scientific texts or bibliographic updates, so as to employ them in different contexts, including research;
- use the acquired know-how to understand the contents of teachings such as laboratory diagnostics, medical clinics, medical therapy
Prerequisites
Students had to know Biochemistry, Cell Biology, Genetics and Phisiology and they must know the principles of the most common laboratory techniques
Teaching methods
Theoretical lectures that will deal with all the topics included in the course program
Other information
The teacher becomes available (by prior arrangement) even outside of official office hours for explanations about the topics of the program
Learning verification modality
The CFUs will be acquired by passing an oral examination (lasting up to 45 min). The exam will then be evaluated in thirty.
For information about services for students with disabilities and/or DSA, visit http://www.unipg.it/disabilita-e-dsa
Extended program
Basic concepts of reliability analytical: precision, trueness, accuracy, sensitivity and specificity, the quality management. The internal quality control. Predictive value and decision-making levels.
Quantities and units of measurement in clinical biochemistry, pre-analytical variability, reference values and biological variability
Principles and techniques for collecting, processing and storage of biological samples.
Point-of-Care testing. Dry Chemistry: principles and its use in the examination of urine and in ambulatory monitoring of blood glucose.
Clinical Enzymology
Biochemical markers of function or damage of tissues or organs with particular reference to: disorders of fluid and electrolyte metabolism and acid-base (blood gas analysis); markers of liver function; lipoproteins; diabetes mellitus and dynamic tests of endocrine function of the pancreas; heme metabolism and jaundice, coagulation tests;. Transmissible spongiform encephalopathies. Clinical biochemistry of exercise
Description of the most used techniques in Clinical Biochemistry: Nucleic acids electrophoresis, Protein electrophoresis, Western-blotting , ELISA assay, RIA assay, Fluorescence Microscopy.- Basic elements of Molecular Biology (genome, gene, mutations, polymorphisms) and utilization of databases (eg. NCBI, Enseble Genome Browser, Globin Gene Server).- Integration between Clinical Biochemistry methods and Molecular Biology methods : Study of Hemoglobinopathies- Description of the most used techniques in Molecular Diagnostics:end-point PCR , Real-Time PCR, dHPLC, DNA sequencing (Sanger method) , NGS (Next Generation Sequencing).
Obiettivi Agenda 2030 per lo sviluppo sostenibile

Condividi su