Unit METHODOLOGIES OF CITOLOGICAL AND HISTOLOGICAL SURVEY

Course
Biology
Study-unit Code
A001032
Curriculum
Biosanitario
Teacher
Ines Di Rosa
Teachers
  • Ines Di Rosa
Hours
  • 47 ore - Ines Di Rosa
CFU
6
Course Regulation
Coorte 2021
Offered
2021/22
Learning activities
Caratterizzante
Area
Discipline del settore biodiversità e ambiente
Academic discipline
BIO/06
Type of study-unit
Obbligatorio (Required)
Type of learning activities
Attività formativa monodisciplinare
Language of instruction
Italian
Contents
Instruments for the cytological and histological analyses: light and electron microscopy. Principles and methods of the biological sample preparation. Histomorphological stains. Technique for identifying the cellular or tissue constituents in situ: histochemical, histoenzymatic and immunohistochemical stains. In situ hybridization. Cytology diagnostics. Histopathology.
Reference texts
Equipements provided by the teaching.
Educational objectives
The main knowledge acquired will be:
- principles and applications of instruments for cytological and histological analyses;
- basic and advanced methods of cytological and histological analyses.

The main competence will be:
- ability to apply instruments for cytological and histological analyses;
- ability to apply methods for cytological and histological analyses on scientific, biomedical and environmental investigations;
- ability to assess the results of cytological and histological analyses on scientific, biomedical and environmental investigations.
Prerequisites
In order to be able to understand the topics of the program, students must have acquired basic knowledge of Cytology, Histology, Anatomy, Molecular Biology.
Teaching methods
The course is organized as follows:
-lectures on all topics listed in the program;
-laboratory on some topics listed in the program. Students will follow 3 tutorials of 4 hours each.
Learning verification modality
The exam includes an oral test consisting of an interview of about 40 minutes long aiming to ascertain the knowledge level and understanding
capability acquired by the student about the topics discussed during the course. The oral exam is also intended to test student synthesis capability and propriety of language.

For information on support services for students with disabilities and / or DSA visit the page.
Extended program
Instruments for the cytological and histological analyses: bright field microscope, dark field microscope, phase contrast microscope, fluorescence microscope, confocal microscope, multiphoton microscope, super resolution fluorescence microscope, transmission and scanning electron microscopes.

Methods of preparation of cytological and histological specimens: fresh specimens, fixed specimens (sampling, chemical and physical fixation, embedding in paraffin wax, epoxy and acrylic resins, sectioning, chemistry of staining, common laboratory stains).

Histomorphological, cytochemical, histochemical, histoenzymatic and immunohistochemical stains. In situ hybridization.

Cytology diagnostics: material collection; slide preparation; sample fixation; staining of the cytology smears; smears examination.

Histopathology: histopathological analyse in diagnosis of infectious diseases and in environmental studies.
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