Unit APPLIED BIOCHEMISTRY METHODS

Course
Pharmacy
Study-unit Code
65105410
Location
PERUGIA
Curriculum
In all curricula
Teacher
Carmela Conte
Teachers
  • Carmela Conte
  • Michela Codini (Codocenza)
Hours
  • 42 ore - Carmela Conte
  • 21 ore (Codocenza) - Michela Codini
CFU
9
Course Regulation
Coorte 2015
Offered
2017/18
Learning activities
Caratterizzante
Area
Discipline biologiche e farmacologiche
Academic discipline
BIO/10
Type of study-unit
Obbligatorio (Required)
Type of learning activities
Attività formativa monodisciplinare
Language of instruction
Italian
Contents
Methods and investigation strategy in clinical biochemistry, nutrition and metabolism of organs and tissues. Main blood and urinary biomarkers used in laboratory diagnostics of infectious, metabolic and age-related diseases, prognostic indicators and therapy monitoring and toxicology investigation.
Reference texts
I principi di biochimica di Lehninger, Nelson & Cox, Zannichelli Ed. (consigliato per nozioni fondamentali di biochimica gen.) - Baynes, J.W., Dominiczak, M.H. Biochimica per le discipline biomediche. Casa Editrice Ambrosiana (2° edizione 2006) (utile per approfondimenti di biochimica medica) - Clinical Chemistry (6th edition), Marshall & Bangert, Elsevier Ed - Clinical Chemistry (5th edition), Kaplan & Pesce, Elsevier Ed - Gaw, R.A. Cowan, D.S.J. O'Reilly, M.J. Stewart, J. Shepherd, Clinical Biochemistry, Churchill Livingstone, (1995) - MEDICINA DI LABORATORIO, SACCHETTI L., UTET Ed, Collana MEDICINA CANALE UNIVERSITARIO - Pasquinelli (vol.1)
Educational objectives
The course will give the students appropriate knowledge on biochemistry methods and their application in the interpretation of the biochemical and molecular bases of diseases. Students will achieve sufficient skilling in the interpretation of clinical laboratory profiles, laboratory methods and analytical procedures used in clinical diagnostics, as well as in pharmacology and toxicology monitoring of patients and human nutrition.
Prerequisites
Solid background on General Biochemistry, Physiology and General Pathology, as well as of pharmacology and toxicology, is needed to follow the course thus achieving a complete understanding of the proposed program.
Teaching methods
Frontal lessons and seminars
Other information
Dr Marta Piroddi and Dr Desirée Bartolini will support in student examination and will take seminars.
Learning verification modality
Profit evaluation will consist of an oral exam to be performed in one session of approx. 20 min. with three or more questions. The student should demonstrate a proficient and flexible knowledge of all main concepts presented during the course, demonstrating good skilling in clinical chemistry methods, and laboratory data interpretation.
Extended program
Teaching program - main topics:
laboratory procedures and techniques in biochemistry and clinical chemistry
Laboratory parameters, data interpretation and reporting
Blood and other biological samples in clinical chemistry
Lab protocols and techniques in biochemistry and clinical chemistry
Hormonal axes and metabolism: the hypothalamic-pituitary axis function regulation factors that affect energy metabolism
Blood tests: blood cells, metabolic and functional tests
Insulin resistance, obesity, diabetes mellitus and metabolic syndrome
Lipid metabolism and lipidomics: lipoproteins and diagnostics of dyslipidemias
Protein metabolism and functions: plasma proteins and clinical proteomics methods
Laboratory investigation of inflammatory syndromes and protein-calorie malnutrition
Urine tests
Risk factors, laboratory tests and nutritional aspects of the athero-thrombotic process
Liver function tests
Kidney function tests and uremic syndrome
Applied molecular biology and clinical enzymology (tumor markers and genetic profiles)
Metabolism and indices of iron, calcium and phosphorous, micronutrients and trace elements
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