Unit LABORATORY OF CHEMISTRY (INTERDISCIPLINARY)
- Course
- Biological sciences
- Study-unit Code
- 55030202
- Curriculum
- In all curricula
- Teacher
- Jenny Desantis
- Teachers
-
- Jenny Desantis
- Hours
- 19 ore - Jenny Desantis
- CFU
- 2
- Course Regulation
- Coorte 2021
- Offered
- 2022/23
- Learning activities
- Altro
- Area
- Altre conoscenze utili per l'inserimento nel mondo del lavoro
- Academic discipline
- CHIM/06
- Type of study-unit
- Obbligatorio (Required)
- Type of learning activities
- Attività formativa monodisciplinare
- Language of instruction
- Italian
- Contents
- Prevention and safety in the chemistry laboratory. Separation and purification techniques of chemical compounds: filtration, extraction, recrystallization, distillation, and chromatography. Determinations of acidity/basicity constants by acid-base titration. Practical exercises in the laboratory are planned.
- Reference texts
- 1. Royston M. Roberts, John C. Gilbert, Stephen F. Martin, Chimica Organica Sperimentale, Zanichelli
2. Teacher lecture notes. - Educational objectives
- The fundamental techniques for the purification and separation of organic compounds and the principles that are the basis of these techniques will be learned along with the determination of the concentration of solutions by volumetric analysis.
By the end of the course, the student should be able to prepare solutions at specific concentration, to determine the concentration of unknown solutions, to perform a filtration, and to perform a thin-layer chromatography and a column chromatography. - Prerequisites
- In order to understand the given topics, a good knowledge of the basic concepts of general chemistry, on the chemical bonding, acidity and basicity, equilibrium constants, is required.
- Teaching methods
- The course is organized as follows:
- Lectures on all subjects of the course
- Practical laboratory exercises. In particular, students will perform four lab experiences:
1) preparation of solutions with a defined concentration;
2) Determination of the concentration of an acetic acid solution by the volumetric acid-base titration;
3) Synthesis of phenyl benzoate: separation, TLC analysis and synthesized compound characterization by melting point;
4) Separation of ortho- and para-nitrophenol by column chromatography. - Other information
- Frequency: Required
- Learning verification modality
- No final examination.
- Extended program
- Prevention and safety in the chemistry laboratory. Separation and purification techniques of chemical compounds. Filtration: simple and under vacuum, vacuum pumps. Extraction: the partition coefficient, selective extraction, discontinuous and continuous extraction. Recrystallization and melting point. Distillation: simple and fractional. Chromatography: theoretical principles, thin layer chromatography, column chromatography, gas chromatography and HPLC. Determination of acidity/basicity constants by acid-base titration. Volumetric analysis. pH indicators.
Practical exercises in the laboratory are planned regarding the preparation of solutions with defined concentration, the acid-base titration, the thin layer chromatography and column chromatography.