Unit DIAGNOSTIC METHODS IN PATHOLOGICAL ANATOMY

MOLECOLAR BIOLOGY'S METHODS AND THECNIQUES IN PATHOLOGICAL ANATOMY

Code	50993201
CFU	1
Teacher	Martina Mandarano
Teachers	Martina Mandarano
Hours	12 ore - Martina Mandarano
Learning activities	Affine/integrativa
Area	Attività formative affini o integrative
Academic discipline	MED/46
Type of study-unit	Obbligatorio (Required)
Language of instruction	Italian
Contents	Application of molecular biology techniques to tissue preparations of Anatomic Pathology. Principles of the different nucleic acid analysis techniques (DNA and RNA extraction, PCR and its variants, main types of sequencing). In Situ Hybridization techniques (FISH, CISH, SISH). Tissue microarrays (TMAs). Main applications of molecular biology techniques, particularly as regards the diagnostic-therapeutic implications in the oncology field.
Reference texts	A. Fassina, R. Cappellesso: Anatomia patologica e citopatologia per TLB. (2019) Cleup G. Pelosi, A. Sapino, E. Maiorano: Manuale di Anatomia Patologica Funzionale. (2022) Edizioni Minerva Medica L. Ruco, A. Scarpa: Anatomia Patologica - Le Basi. (2007) UTET
Educational objectives	Acquisition of the principles for the adequate preparation of histological and/or cytological samples for the application of molecular biology techniques. Acquisition of the principles and methodologies related to molecular biology techniques applied to Anatomic Pathology and Histology. Learn to recognize the critical points of the various stages of a method and to obtain reliable results for a correct application of molecular biology techniques.
Prerequisites	In order to understand the most important aspects of molecular biology techniques with the related anatomo-clinical correlations and associated problems, the student must have basic knowledge of Biology, Basics of Genetics, Histology, Human Anatomy, Methods and techniques of histology and cytology, Molecular Biology, Human Pathology Sciences.
Teaching methods	Taught lessons. Practical activity at the laboratory of Anatomic Pathology and Histology. There are courses, materials and/or assessment methods consistent with the needs of students with disabilities and / or SLDs.
Other information	Office hours: Thursday 15-17 (by appointment), also through video call on the Teams platform. e-mail address: martina.mandarano@unipg.it Phone number: +39 075 5782316
Learning verification modality	Oral exam The exam relating to this module includes an oral test of about 10 minutes which will focus on a question relating to theoretical topics indicated in the program. The purpose of the oral exam is to verify the level of knowledge reached by the student on the theoretical contents indicated in the program, in order to evaluate the student’s ability to present them, with language properties and terminological appropriateness, as well as student’s problem-solving skills, related to the use of molecular biology techniques in the Anatomic Pathology field. There are courses, materials and / or assessment methods consistent with the needs of students with disabilities and / or SLDs. For information on support services for students with disabilities and / or SLDs, visit the page http://www.unipg.it/disabilita-e-dsa
Extended program	Introduction: bases of molecular pathology Definition of molecular pathology Aims of molecular pathology Basic definitions useful in molecular pathology Molecular pathology techniques and the importance of their knowledge by the biomedical laboratory technician Preparation of histological and cytological samples for the application to molecular biology techniques Types of biological materials which can be used in molecular biology and biological materials more frequently used in the Anatomic Pathology laboratory Pre-analytical phase for molecular biology analyses Role of the laboratory technician of Anatomic Pathology in the preparation of materials suitable for molecular biology techniques Principles of the different techniques of nucleic acid analysis Anti-contamination devices Nucleic acid extraction PCR (polymerase chain reaction) and its phases Nested-PCR RT-PCR qRT-PCR Kind of probes used in PCR PCR after treatment with bisulfite Classic sequencing Pyrosequencing Sequencing by mass spectrometry - MALDI-TOF NGS (Next Generation Sequencing) In situ hybridization (FISH, CISH, SISH) Tissue Micro-Array Preparation Applications Applications of molecular biology techniques Applications to the microbiological field Applications to the diagnostic field Applications to the oncology field, with particular regards to breast, ovarian, colorectal carcinoma, lung adenocarcinoma Applications to the therapeutic field

HYSTOPATHOLOGI'S NOTIONS AND ELEMENTS OF AUTOPSY

Code	50666802
CFU	2
Teacher	Angelo Sidoni
Teachers	Angelo Sidoni
Hours	24 ore - Angelo Sidoni
Learning activities	Caratterizzante
Area	Scienze e tecniche di laboratorio biomedico
Academic discipline	MED/08
Type of study-unit	Obbligatorio (Required)
Contents	Basic histopathological aspects of acute and chronic inflammations, cell degenerations, disorders of cell growth, differentiation and maturation. Dissection protocols and organ removal modalities during autopsy.
Educational objectives	The student should acquire competence in the main activity of the histopathology laboratory

ORGANIZATION OF ANATOMIC PATHOLOGY LABORATORY

Code	50787801
CFU	1
Teacher	Giamprimo Bartoli
Teachers	Giamprimo Bartoli
Hours	12 ore - Giamprimo Bartoli
Learning activities	Caratterizzante
Area	Scienze e tecniche di laboratorio biomedico
Academic discipline	MED/46
Type of study-unit	Obbligatorio (Required)
Language of instruction	Italian
Contents	Organizzazione del Laboratorio di Isopatologia, competenze e responsabilità del TLB che opera nell'ambito dell'Anatomia e Istologia patologica
Reference texts	J.D. Bancroft M. Gamble, Theory and Practice of Histological Techniques, Churchill Livingstone Elservier, Sixth Edition - L. Roco A. Scarpa, Anatomia Patologica Le Basi, UTET.
Educational objectives	Attraverso il complesso delle lezioni teoriche e pratiche lo studente potrà acquisire le conoscenze relative alla organizzazione e alle competenze necessarie ad affrontare con responsabilità e piena autonomia professionale le attività previste in un moderno laboratorio di Anatomia ed Istologia Patologica. Lo studente: - analizzerà i principali processi tecnici per lo sviluppo della Diagnosi Istopatologica, dovrà acquisire conoscenza delle linee guida, procedure, istruzioni operative, controllo qualità e norme di sicurezza che caratterizzano il laboratorio di Anatomia e Istologia Patologica.
Prerequisites	Nozioni e fondamenti derivanti dallo studio propedeudico delle metodiche e tecnihe di istologia
Teaching methods	Il corso prevede lezioni frontali ed è organizzato con forte impronta pratica. Gli argomenti trattati con confronti pratici da affrontare direttamente nel laboratorio.
Learning verification modality	L'esame orale verrà sostenuto collegialmente da tutti i docenti afferenti al corso di "Metodologie diagnostiche di Anatomia Patologica.
Extended program	Modalità di invio, trasporto e conservazione dei campioni al laboratorio di Anatomia e istologia Patologica. Preparazione dell'esame istologico su preparati inclusi in paraffina e su preparati sezionati al criostato. Corretta esecuzione di campioni per riduzione da pezzo operatorio. Orientamento dei campioni all'inclusione in paraffina e allestimento dei preparati. Trattamento di particolari campioni "critici": piccole biopsie, agoaspirati, biopsie cutane. Controllo qualità e sicurezza.

PATHOLOGICAL ANATOMY AND HYSTOLOGY'S TECNIQUES

Code	50657002
CFU	2
Teacher	Guido Bellezza
Teachers	Guido Bellezza
Hours	24 ore - Guido Bellezza
Learning activities	Caratterizzante
Area	Scienze e tecniche di laboratorio biomedico
Academic discipline	MED/08
Type of study-unit	Obbligatorio (Required)
Language of instruction	Italian
Contents	Main histological, histochemical and immunohistochemical techniques. Quality control of immunohistochemistry.
Reference texts	"Anatomia Patologica. Le basi." L. Ruco; A. Scarpa UTET Edizioni Scienze Mediche, "Theory and Practice of Histological Techniques" 5th edizione, JD Bancroft; M. Gamble, Churchill Livingstone
Educational objectives	Knowledge of managing histopathological laboratory. Main histological, histochemical and immunohistochemical techniques.
Teaching methods	face-to-face - Practical training - Theoretical lessons and practical training - Laboratory- There are courses, materials and / or assessment methods consistent with the needs of students with disabilities and / or SLDs.
Other information	Office hours: Tuesday 16-18 (by appointment), also via video call mode on the Teams platform. email: guido.bellezza@unipg.it phone: + 39 75/5783891
Learning verification modality	Oral examination (1 question for each module of the course). There are courses, materials and / or assessment methods consistent with the needs of students with disabilities and / or SLDs. http://www.unipg.it/disabilita-e-dsa
Extended program	Histochemical stains: connective tissues and stains; demonstration of mucins, lipids and nucleic acids, pigments and minerals. Immunohistochemical techniques. Theory. Mono and polyclonal antibodies. Labels. Immunohistochemical methods: traditional direct technique and indirect technique. Avidin-biotin technique. PAP and APAAP. Polymer. Immunoperoxidase and immunophosphates techniques. Antigen retrieval techniques. Blocking endogenous enzymes. Quality control of immunohistochemistry.

CYTOPHATOLOGY'S NOYIONS AND TECHNIQUES

Code	50667302
CFU	2
Teacher	Rachele Del Sordo
Teachers	Rachele Del Sordo
Hours	24 ore - Rachele Del Sordo
Learning activities	Caratterizzante
Area	Scienze e tecniche di laboratorio biomedico
Academic discipline	MED/08
Type of study-unit	Obbligatorio (Required)
Language of instruction	Italian
Contents	Methods for collection, retention, fixation of cytological specimens. Processing and staining techniques.
Reference texts	1) "Anatomia Patologica. Le basi." L. Ruco; A. Scarpa UTET Edizioni Scienze Mediche 2) "Theory and Practice of Histological Techniques" 5th edizione JD Bancroft; M. Gamble Churchill Livingstone There are courses, materials and / or assessment methods consistent with the needs of students with disabilities and / or SLD ". For information on support services for students with disabilities and / or SLD, visit the page http://www.unipg.it/disabilita-e-dsa
Educational objectives	Known how to send the cytologic material, the various techniques of processing of the material and stains. The main skill will: obtain a optimal cytologic slide for an accurate diagnosis.
Teaching methods	Lessons face-to-face - Practical training at cytology laboratory. Observation at light microscopy of cytologic slides There are courses, materials and / or assessment methods consistent with the needs of students with disabilities and / or SLD ". For information on support services for students with disabilities and / or SLD, visit the page http://www.unipg.it/disabilita-e-dsa
Other information	For information rachele.delsordo@unipg.it Reception hours: Wednesday 15:00 p.m.-18:00 p.m.
Learning verification modality	Oral questions There are courses, materials and / or assessment methods consistent with the needs of students with disabilities and / or SLD ". For information on support services for students with disabilities and / or SLD, visit the page http://www.unipg.it/disabilita-e-dsa
Extended program	Historical overview. Diagnostic cytology. Cytologic sampling techniques: exfoliative cytology, abrasive cytology, aspiration cytology. Advantages and disadvantages respect to histopathology. The role of technician in cytopathology laboratory. Treatment and secure of cytological samples. Fixation, processing and staining techniques. The Papanicolaou stain. Other stains useful in diagnostic cytology. Mounting the cell sample. Immunocytology. Cell-block Diagnostic cytology of female genital tract. The normal female genital tract. Benign disorders and neoplastic lesions. PAP test. Its role in screening programs.