Unit AGRICULTURAL MICROBIOLOGY

Course
Agricultural and environmental sciences
Study-unit Code
80005206
Curriculum
In all curricula
Teacher
Gianluigi Cardinali
Teachers
  • Gianluigi Cardinali
Hours
  • 54 ore - Gianluigi Cardinali
CFU
6
Course Regulation
Coorte 2021
Offered
2022/23
Learning activities
Caratterizzante
Area
Discipline della produzione vegetale
Academic discipline
AGR/16
Type of study-unit
Obbligatorio (Required)
Type of learning activities
Attività formativa monodisciplinare
Language of instruction
Italian
Contents
Cellular organization of eukaryotic microorganisms. Systematic and taxonomy of eukaryotic microbes. Eukaryotic models and genetic organization types. Principles of genetic improvement and principles of applications in microbial biotechnologies. Examples of microbial biotechnological applications.
Reference texts
B. Biavati, C. Sorlini. Microbiologia Agraria ,2007, Ed. Ambrosiana, Milano 
J.J. Perry, J.T. Staley, S. Lowry Microbiologia, 2004. Zanichelli. Milano 
Dispense integrative distribuite durante il corso 
M.T. MADIGAN, J.M. MARTINKO & J. PARKER, Brock, Biologia dei Microrganismi, 
2003, Casa Editrice Ambrosiana, Milano
Educational objectives
Understanding the quantitative and the biotechnological approach in microbiology
Prerequisites
mathematics, includdingt at least linear equation systems and logarithmschemistrycell biology
Teaching methods
lectures and practicals
Other information

Learning verification modality
written exam to access to the oral exam noramlly articualted in  three questions:1. technical microbiology2. general microbiologi3. applied  microbiologythe technical microbiology part is essential for the prosecution of the exam
Extended program
Cellular organization of eukaryotes 
Structure and functions of the various components of the cell. The sexual and asexual reproduction. 
Principles of systematic microbial eukaryotes. The concept of species in microorganisms 
eukaryotes and their classification. Molecular taxonomy and biotechnological implications 
Yeasts as a model of microbial eukaryotic cell. Elements on the cultivation of microbial eukaryotes. 
Genetic organization of the various types of microorganisms. Genomic organization of the various classes of microorganisms. Systems of vertical transmission of genetic information. Horizontal gene transfer systems. 
Control of gene expression in eukaryotic microbes, the system GAL / LAC yeast, cloning using Two Hybrid System. 
Genetic improvement. Key strategies and their choice. Direct Selection, reversed overnight. Biodiversity research and the variability in nature. Mutagenesis. Protoplast fusion. Molecular Cloning. 
Case studies of biotechnology applications.
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