Unit ADE - Microscopic examination of cells and tissues: techniques and methods
- Course
- Veterinary medicine
- Study-unit Code
- A000671
- Curriculum
- In all curricula
- Teacher
- Luisa Pascucci
- Teachers
-
- Luisa Pascucci
- Hours
- 25 ore - Luisa Pascucci
- CFU
- 1
- Course Regulation
- Coorte 2014
- Offered
- 2018/19
- Learning activities
- A scelta dello studente
- Area
- A scelta dello studente
- Academic discipline
- VET/01
- Type of study-unit
- Opzionale (Optional)
- Type of learning activities
- Attività formativa monodisciplinare
- Language of instruction
- Italian
- Contents
- 1. Behavioral rules in a laboratory of histology: personal and
environmental security management.
2. Histological techniques: preparation of cell and tissue specimens for
cytomorphological/histomorpological, cyto/histochemical, immuno
cyto/histochemical analyses. Principles of immunofluorescence.
3. Principles of electron microscopy: preparation and observation of
samples for electron microscopy.
4. Morphological study of cultured cells. How to work in a cell culture
laboratory: staff flow, behavioral rules, cleanliness and sterility
management. Cellular isolation and cell culture manipulation. Preparation
of cells for microscopic observation. - Reference texts
- Microscopie in biologia e medicina
Cesare Scala,Gianandrea Pasquinelli,Giovanna Cenacchi
Editore: CLUEB - Educational objectives
- At the end of the course, the student will know how to process cytological
and histological specimens for microscopic observation and will be able
to apply this knowledges to specimen preparation. - Prerequisites
- None
- Teaching methods
- The course is exclusively practical so the lessons will be held in the
laboratory and will cover the topics detailed in the program. - Learning verification modality
- Oral
- Extended program
- 1. Histological techniques:
- behavioral rules in a histology laboratory; personal and environmental
safety management (1h);
- preparation of cell and tissue specimens for cyto/histomorphological,
cyto/histochemcal, immuno cyto/histochemical analysis (8h);
- principles of immunofluorescence (3h).
Modalità di verifica
dell'apprendimento
Orale
Per informazioni sui servizi di supporto agli studenti con disabilità e/o DSA
visita la pagina http://www.unipg.it/disabilita-e-dsa
Programma esteso 1. Tecniche di studio in istologia:
- norme comportamentali in un laboratorio di istologia: gestione della
sicurezza personale e ambientale (1h);
- allestimento di campioni cellulari e tissutali per analisi
cito/istomorfologiche cito/istochimiche immuno-cito/istochimiche (8h);
- Principi di immunofluorescenza (3h).
2. Principi di microscopia elettronica:
- allestimento di preparati per l'osservazione al microscopio elettronico
(5h).
- Osservazione di preparati al microscopio elettronico a trasmissione
(TEM) e/o a scansione (SEM) (2h)
3. Studio morfologico di cellule in coltura:
- come si lavora in un laboratorio di colture cellulari; flusso di personale,
norme comportamentali, pulizia e gestione della sterilità (1h);
- isolamento cellulare e manipolazione di colture cellulari (3h);
- preparazione di cellule per l’osservazione al microscopio (2h).
2. Principles of Electronic Microscopy:
- preparation of samples for electron microscopy (5h);
- observation at Trnsmission (TEM) and /or scanning (SEM) electron
microscope (2h);
3. Morphological study of cultured cells:
- how to work in a cell culture lab; staff flow, behavioral rules, sterility
cleanliness and management (1h);
- cell isolation and cell culture manipulation (3h).
- preparation of cells for microscopic observation (2h).